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mmu_ciZfp207_648
Basic information of circular RNA
PanCircBase ID:
mmu_ciZfp207_648
mm39_Chr. Position ID:
chr11|80286399|80287047|+
Gene name:
Zfp207
Transcript:
ENSMUST00000123726.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
9
Exon/intron offsets:
0
Genomic length:
648
Exon/intron sizes in nt:
648
Flanking exon/introns:
chr11:80286399-80289241
Splice length:
648
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTTGGAGATTAAACCTTTTCTCAACTTGTGCTGTTTATATAGACAAGCTTCCGTCATTAAGGCTTCATTGTGACTTTTAACAAACATAATCTTCCCACATACCAGGAACTATTGGACATTTATTTGACATGGGAGAAATTATTTGGAATAATAAAACAGGAACTTTTCCTGAAGTTGCAATTTATACTGTATGGCTTCTTTTTCATGTTTCATCTAGGTTTTTAGAAGTGAAGTATAGTAAATTTGGTTCGTTAAATTGTGAAGGCGCTGGAATTACATGAACATACCACCTTAAATAAAGGCAAGTTCTGTAAGATTACATTGCTATTTGTAAAGTTATGCCTTCGCAGCATTTCCAATGCTGTTGGACTTCATGTCCCCAACCTAGCTTGGTGAGGGTTGTAACTGTTTCCAAGTACTTGTACATTGGAAGTTTGAATGTGTAACAATATTTAATGTAATTAGAGAGAGTTCCTCCTGTTGCAGGGTTTAAGAAAACGGGCCCATTATTATGGTCATGTGACTTTTCTGTACTGTTAAACTTCATTGTAATAAAATGAGACAAAAATTTATGCCTTTTTATTCATAACCCAGCTGTGGACCACTGCCTGAAAGGTTTGTATGGATGCATGCCACGGTAGATGTCC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTGTGGACCACTGCCTGAAA
60.18
TGTTTGTTAAAAGTCACAATGAAGCC
59.47
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCTTTTTATTCATAACCCAGCT
58.98
TGAAGCCTTAATGACGGAAGCT
60.03
143
PCR template sequence:
TGTAATAAAATGAGACAAAAATTTATGCCTTTTTATTCATAACCCAGCTGTGGACCACTGCCTGAAAGGTTTGTATGGATGCATGCCACGGTAGATGTCCGTTTGGAGATTAAACCTTTTCTCAACTTGTGCTGTTTATATAGACAAGCTTCCGTCATTAAGGCTTCATTGTGACTTTTAACAAACATAATCTTCCCACA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-181a-5p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-181b-5p(1), mmu-miR-494-3p(1), mmu-miR-34b-5p(1), mmu-miR-224-5p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACGGTAGATGTCCGTTTGGAGATTAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIZFP207_648_ORF_5
0.432
-0.193330936
0.010274625
96
ORF sequence:
ATGCATGCCACGGTAGATGTCCGTTTGGAGATTAAACCTTTTCTCAACTTGTGCTGTTTATATAGACAAGCTTCCGTCATTAAGGCTTCATTGTGA
Peptide sequence:
MHATVDVRLEIKPFLNLCCLYRQASVIKASL
IRESfinder result
IRESfinder score
0.802229016
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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