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mmu_ciWsb2_591
Basic information of circular RNA
PanCircBase ID:
mmu_ciWsb2_591
mm39_Chr. Position ID:
chr5|117514863|117515454|+
Gene name:
Wsb2
Transcript:
ENSMUST00000031309.16
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
8
Exon/intron offsets:
0
Genomic length:
591
Exon/intron sizes in nt:
591
Flanking exon/introns:
chr5:117514863-117515454
Splice length:
591
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATGTATGTGCTTTGAACAGATGGGCAGACTCTGTGTGCACCATGAAGGAAGTGTGGAGAACAGTAATGCCATTTATAGATGTTAGTAATCAATGTAAAGGCAAGGAGAGACCTGTATGTGGTCCATGTTACCTGAATGGCCAACTCCACAAGGAGGCATAAGATACGATGATGCTGTTTTTATCTGGGAAGTCTACAGCTGAGGTCACCTTACAGATCACATCTATAATTGGTACATGGCCGTTATTGTGTGAGCTCAGGCCCTTCTCGTGCAAGCCTCTTGTCATCAAAGGTGTTTTTGTTGTTGGTTTTGGACAATCTTAAACCTCACTGGTCTTGAGTTCCTGGGCTTGGGTTCTCATCCCACTTTAATCTGATGAACCAGCTGGGACCACAGGCACAGGTCACAAAATCCTGGGTTCTAACAATCCTACCAAACAGCAGGCATTACCAGCAGTCTGCAGCATTTCCCAGTCAGGGCCCACTCCAGCTTTTGGAGTCTCCATTTCAGGTCATCCCAGAATCACCACTGTCCATTATAATGAAAACAGCTCTCTGGGGCTGCTAAGCACTGGTCTCTGTTGTTTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGGTCATCCCAGAATCACCA
59.44
CCACACTTCCTTCATGGTGC
59.12
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGGTCATCCCAGAATCACC
59.16
CCACACTTCCTTCATGGTGC
59.12
141
PCR template sequence:
CTTTTGGAGTCTCCATTTCAGGTCATCCCAGAATCACCACTGTCCATTATAATGAAAACAGCTCTCTGGGGCTGCTAAGCACTGGTCTCTGTTGTTTCAGGTATGTATGTGCTTTGAACAGATGGGCAGACTCTGTGTGCACCATGAAGGAAGTGTGGAGAACAGTAATGCCATTTATAGATGTTAGTAATCAATGTAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-31-5p(1), mmu-miR-205-5p(1), mmu-miR-15b-5p(1), mmu-miR-320-3p(1), mmu-miR-758-3p(1), mmu-miR-342-3p(1), mmu-miR-377-3p(1), mmu-miR-335-3p(1), mmu-miR-3065-5p(1), mmu-miR-3061-5p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCTGTTGTTTCAGGTATGTATGTGCT
siRNA sets
ATACATACCTGAAACAACA, CATACATACCTGAAACAAC, TACATACCTGAAACAACAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.676030312
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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