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mmu_ciWac_1253
Basic information of circular RNA
PanCircBase ID:
mmu_ciWac_1253
mm39_Chr. Position ID:
chr18|7916297|7917550|+
Gene name:
Wac
Transcript:
ENSMUST00000171042.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
7
Exon/intron offsets:
0
Genomic length:
1253
Exon/intron sizes in nt:
1253
Flanking exon/introns:
chr18:7916297-7917564
Splice length:
1253
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGAATACCCATACTTACAGTTTTTTGAACATTATTACCTGCATTATACAAATGTATTTATAAAATTCTCAACTCAAGGAATGACTCTTAGTTTTCTTTACAAATTTATGTTGAAATTTTCTACTTTAGTATGTCCACCTCCAATGTGCTTGGTTGTATAATTGAGTTAGTAGTGTTTGTAGACATTTTCAAGTCTATTAATATGAAGATCTTAGAAATACTAAAAGTAAGCTACTCAGTAAAACTGCTAGTGATTTTTGTTTTGTTTTTAGTAGTGTTTTTGTTTGTTTAATCATATGAATTTGATATATACATTATATTTATTATTATTATTACTATTATTATTTTACTGTTTCTAAAGTATTTGAACCAACACCTCATAGTACCCTGAGCAAAAATGGATTTTGTTTTAATTTAAAGCCGTTACAATTCAAATGTTAAATCTCTATTTATGCATTTTCTCATAATAGAAGATCTTAAGTGGCCTTTATTCATCCAAAAGTTGGAGCCTTCTCACACTTCTTGTTTTTCTTGTTACTCTCAACTTTAGTGTATTTATTTTATATTATTTATAACTGTAATTCTTTACTTTTTTTCTTTTTTGGCTAAATGTTATATAAACCGTCTGAAATTTTATAAAGCCGTCTGTAAAAAATTATTCTTAGGCATCAAAAATACATGGTATTCCTAAATGCCACCTGTTAGTATCCCTTTTAAAGACATGGTGAGTATTGATGTACAGGGCTTTATTGGTCAGTTTGTTAAAAATGGTCTTGTGAAACAGCATCCATTTCTTCAGTCTTGCTAATAAAGCACCAAGGAGTAGCATGCATAGGCTCCTGCTGCTCTTAACATGTCTTAGCAGCTTTAGAGTTGACCTTTATTGTTAGCCTGTTCTCTTCCTTTGACCATTTCAAGGCTTTTAAAAAATATATGAAATTTATTTATTTTTATGTGCATATGTGTTATGCCTGCATTTTTTTTTCTTCTATTGTGAAGGGGTCAGATCCTGTAGTTACAGCTATTTGGGTGCTCGGAATTGAACCTGGGTTGGGTCCTCTGGAAGAGTGCTCTTAACCACTGTCTCATTTCTCCAGCCCCCATTTCTTTGTGCTCATTGATTTATTGTTTGTGCTAAGCTTTCATTTTAAAAATTTCATGAAACCAGTTTGTACTTTTACTATTGCTGTTTGTTACCTATGAGTTTAAGTAGTGTAAGATGAATTTGTTTTGATTGGATTTTGAATGTTTTA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTGTTTGTTACCTATGAGTT
55.66
AGAGTCATTCCTTGAGTTGAGA
56.49
157
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTGCTGTTTGTTACCTATGAGT
55.66
AGAGTCATTCCTTGAGTTGAGA
56.49
158
PCR template sequence:
ATTTCATGAAACCAGTTTGTACTTTTACTATTGCTGTTTGTTACCTATGAGTTTAAGTAGTGTAAGATGAATTTGTTTTGATTGGATTTTGAATGTTTTAGTGAATACCCATACTTACAGTTTTTTGAACATTATTACCTGCATTATACAAATGTATTTATAAAATTCTCAACTCAAGGAATGACTCTTAGTTTTCTTTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-203-3p(1), mmu-miR-495-3p(1), mmu-miR-29b-1-5p(1), mmu-miR-301b-3p(1), mmu-miR-224-5p(1), mmu-miR-344d-3p(1), mmu-miR-193b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTTGAATGTTTTAGTGAATACCCATA
siRNA sets
TATGGGTATTCACTAAAAC, ATGGGTATTCACTAAAACA, GTATTCACTAAAACATTCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.956670029
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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