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mmu_ciTrip12_1992
Basic information of circular RNA
PanCircBase ID:
mmu_ciTrip12_1992
mm39_Chr. Position ID:
chr1|84722906|84724898|-
Gene name:
Trip12
Transcript:
ENSMUST00000189670.7
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
1992
Exon/intron sizes in nt:
1992
Flanking exon/introns:
chr1:84722891-84724898
Splice length:
1992
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGGACCATTACCTGGGTATGTCCACTTGCCACCAGAATTGTGAAGGAAAACGTGGTGAGAGGTCTGGTAAGGTGTTGGTCTTTTTTAGGAACAGTGTTCTGTAATGACTTAAGTAATTTGTGTCAATTAATTAAATGCATTTTTTTATTTAAGATCATGCACTGTATTTGATCTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGACAAGGTTTCTTTGTGTACCTCTGGCTGTCCTGGAACTCAGATCAGCCTGCATCTGCCTCCCAAGTGCTAGGTGTGACACCATGCTCAGCCTATACCATGTATTTTAACAACTGATTTTTTGTTTGTTAATTTTGTTTTTGTTTTGTTAATATTTTGTCCTTGGCTGTTCTAGAAGGCTGTTCTATAGAAGCTTTCTCTATAGCTCAGGCTGTCTTTAAACTTACAGAGATCAACCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGACATGTACCACAACTGCCTGGCTTACAACTATTATTTTTTAAAATCAGTAAATCAGTGACTGGAAAGACAAACATTCAAAATTTCTGGTTTTTGTTTTTCGAGACAGGATTTCTCTTTATAGCCCTGGCTGTCCTGGAACTCACTTTTTAGACCAGGCTGGCCTCGAACTTAGAAATCCACCTGCCTCTGCCTCCGGAGTGCTGGGATTAAAGGTGTGCATCACCATGCCCAACACATTAAAAATTTCTAAGTAGATATGTTTTAATATGTAATGATTCTCTTAATTTTGCTTTTGTATATTGGCAATAAAATATTCTGACTCTTTTGATCAGTTCTATTTTGATGGGTATGTAAGTATCCATTTTCAAGATTGATTTCCAATAAAAATTTGTTATTCTTACTGGTTTTAAGGACTTGTAAGATACTAGAGACAGGTAAAATTAATTTGTTAGGGAATTCTGTAAGCTTTCTGGAGTATTTTTATTGAAAATATAATGTAAATTAGTCCACATTCTCACTTTTACAATTTTGAATAAAAACAGTATCTATATTCCTTCACAGTATGACTGAAGTTGTTTTCTACTATCTTAGAATAGTTTTATGAAACAAAGGGACATCACAAAATATGGTGGAATACCTGTAATCCAAGGAAGTTCCCAAGGAGGGGGGAATATTGTTTTTTTGTTTTGTTTTGTTTTTTTACTTTAGATTTTACTTACAAATAAAAACATAATACAAAATAAAAACTAACCTTCAGTTTACTTTGCTCCATCCCTTTAAATTAATAGCTATAAGGAGGGGGAAATGGATTTTGGAATTCAAAGCTAGACTGGGCTATTTAAACTAAAAAACATAAACCAAACCCAAAAAAGCCTGAGGCTTTCCATTTTGTCTAAGTTCATATGAATTATGCTTGCTTAATATTTACATGCTCCTTGGAGTTGTCTAAAATAACTACATGTTAAGTTTGACAGTATCTGACTTGTTAGAACCTTTTACCAGGAAGAAATATTTAAAAGTATGACATTTGTTCAAAAGTATGTAGCAAAGTAAATTTGAATAATTACTTTTTATATTACTAAATTATGTTTTTATTTTTATAAGCCAAAAAATAGAGGATTCTTCCCCCCATTGGTCTTTGAATAGAATTTAGTAGGGAAGGATAAAATACTGCAGAGGTGAGTGTGCCCAGATAGTTGAAGAGTTCTTATTTCCAGAGGAATAATCCAGTAGGTACAGCATTTCATTGAGGTCAGTTTTTATAACACCCTTGATAACATGAATTCACAGTGGCTAGAGATCATGTTCATTTCATGCTTTTCATATGCAGAAAAACCGACTCATTTAGTTATTTGTAATAACTTGTATTGAGTAATTTGAGTTGTTGATGAATCTTAAATAAGTTGACTTTGAAAATAATAGAATAACCTTTTTTCGTAAATTATCTTGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGAGTAATTTGAGTTGTTGATGAATCT
57.71
TACCAGACCTCTCACCACGT
59.89
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTGAGTAATTTGAGTTGTTGATGAA
55.68
TACCAGACCTCTCACCACGT
59.89
156
PCR template sequence:
TTTGTAATAACTTGTATTGAGTAATTTGAGTTGTTGATGAATCTTAAATAAGTTGACTTTGAAAATAATAGAATAACCTTTTTTCGTAAATTATCTTGAGGTAAGGACCATTACCTGGGTATGTCCACTTGCCACCAGAATTGTGAAGGAAAACGTGGTGAGAGGTCTGGTAAGGTGTTGGTCTTTTTTAGGAACAGTGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
17
Interacting miRNAs (#binding sites)
mmu-miR-709(3), mmu-miR-298-5p(2), mmu-miR-203-3p(2), mmu-miR-466b-3p(2), mmu-miR-16-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-223-3p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-152-3p(1), mmu-miR-495-3p(1), mmu-miR-466d-3p(1), mmu-miR-342-3p(1), mmu-miR-377-3p(1), mmu-miR-7a-1-3p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAATTATCTTGAGGTAAGGACCATTA
siRNA sets
TAATGGTCCTTACCTCAAG, AATGGTCCTTACCTCAAGA, ATGGTCCTTACCTCAAGAT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.949147909
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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