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mmu_ciTomm70a_495
Basic information of circular RNA
PanCircBase ID:
mmu_ciTomm70a_495
mm39_Chr. Position ID:
chr16|56957170|56957665|+
Gene name:
Tomm70a
Transcript:
ENSMUST00000166897.3
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
4
Exon/intron offsets:
0
Genomic length:
495
Exon/intron sizes in nt:
495
Flanking exon/introns:
chr16:56957170-56958392
Splice length:
495
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAATTAATGTGAGTTGGTAATAGTTGAATGCTGCTTGAACCAAGACATACTGTGTATGATAACTGAAATTAAAACTGGCTTGTGTTTCTTTTTCCTATACTCTTTCGTTGTTATAGCATATCAATTTTCAGTAAGTCTAAAACTGTACTGAAAGAAACATTTTACGTATTAGGAATTGTCACTCAACAGCCCAAAGATCAAATCCAATCTGCTTCCTATATATTAGCTGCTTGCATATTGCTTGGGGCTATTTTGTGCTGCTACAACATAGTTGAGTGGTTGTTACAGAATTCAAAGTCTATGTGCTATATAATGGCTAAACTGTCTATCACCTGAACTTTTGTAGAAAAGTACTTTCCAGCTCTTGCTCTGTATCATTAGTTTTTAAAGGTAAATTATATCAATGAACATTAGTGACATAATTTAATTCAACAGAGCAATGGATACAGCTATGTGGTCCCAGATCTAAGCTGGTGACTCTTACAATATAAGT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGCTATGTGGTCCCAGATC
59.23
AGGAAAAAGAAACACAAGCCAGT
59.23
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGCTATGTGGTCCCAGAT
58.11
AGGAAAAAGAAACACAAGCCAGT
59.23
146
PCR template sequence:
AATTATATCAATGAACATTAGTGACATAATTTAATTCAACAGAGCAATGGATACAGCTATGTGGTCCCAGATCTAAGCTGGTGACTCTTACAATATAAGTGTAAATTAATGTGAGTTGGTAATAGTTGAATGCTGCTTGAACCAAGACATACTGTGTATGATAACTGAAATTAAAACTGGCTTGTGTTTCTTTTTCCTAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(2), mmu-miR-15a-5p(2), mmu-miR-93-5p(1), mmu-miR-103-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-194-5p(1), mmu-miR-107-3p(1), mmu-miR-3087-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTACAATATAAGTGTAAATTAATGTG
siRNA sets
TTAATTTACACTTATATTG, TAATTTACACTTATATTGT, ATTAATTTACACTTATATT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.880276018
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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