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mmu_ciTmem94_532
Basic information of circular RNA
PanCircBase ID:
mmu_ciTmem94_532
mm39_Chr. Position ID:
chr11|115682002|115682534|+
Gene name:
Tmem94
Transcript:
ENSMUST00000093912.11
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
14
Exon/intron offsets:
0
Genomic length:
532
Exon/intron sizes in nt:
532
Flanking exon/introns:
chr11:115682002-115682572
Splice length:
532
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGAGGAGGGAGGCAGGGTGGAGAAGCCACCGTGTCTGTCATGTGCCTTTGTGCCTCCCTGCCAGAGTAAAACAGCTCAGTTTGAGCCTTTGCAAGAGTCCTGGGCAGCCCTCCTTCCTGTAGCTTCAGACCTGATCTGCGGGATGACCCTGAGGTTTGACCTGAGGCTTCTCTGGGATGGGACTTTGTCCCCTTCCAGTTCTTCCCTGGGTTAGCAGCCTCCTAGTTAGGATCTTCTAAACACATATAGTTATAAGTCCCTGTCAGGTGCTGTCATCCCTGGTCAACACAGACCCCTTGTCACCTCCCTGTATGGGCACTGCCAGGCCTGTTGGATATAGGTCCTATTATTGATTTTAACAAAAAGGCCGGCGATAATTGGTACAGATCCTAAATAGAAGCCGGAGGCCACGATGAGGCTCTCAGCAGTCACAGGAGAGTGGGCGCCAGCCCCCTTCTGAGCATGGCGGGCTGTCTTGTGAGGCTAGTTCTGAGCCTGCTTGGGTATCAGAGCCTTCCCCTGCTTCCTC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGCTGTCTTGTGAGGCTAG
60.11
GCTGTTTTACTCTGGCAGGG
58.83
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGGCTGTCTTGTGAGGCTAG
60.11
GCTGTTTTACTCTGGCAGGGA
60.27
140
PCR template sequence:
CACAGGAGAGTGGGCGCCAGCCCCCTTCTGAGCATGGCGGGCTGTCTTGTGAGGCTAGTTCTGAGCCTGCTTGGGTATCAGAGCCTTCCCCTGCTTCCTCGTAAGAGGAGGGAGGCAGGGTGGAGAAGCCACCGTGTCTGTCATGTGCCTTTGTGCCTCCCTGCCAGAGTAAAACAGCTCAGTTTGAGCCTTTGCAAGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-124-3p(1), mmu-miR-34a-5p(1), mmu-miR-127-5p(1), mmu-miR-207(1), mmu-miR-484(1)
siRNAs for circular RNA silencing
Junction sequence
CCCCTGCTTCCTCGTAAGAGGAGGGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CITMEM94_532_ORF_1
1.2255
-0.19806657
0.082169757
192
ORF sequence:
ATGGCGGGCTGTCTTGTGAGGCTAGTTCTGAGCCTGCTTGGGTATCAGAGCCTTCCCCTGCTTCCTCGTAAGAGGAGGGAGGCAGGGTGGAGAAGCCACCGTGTCTGTCATGTGCCTTTGTGCCTCCCTGCCAGAGTAAAACAGCTCAGTTTGAGCCTTTGCAAGAGTCCTGGGCAGCCCTCCTTCCTGTAG
Peptide sequence:
MAGCLVRLVLSLLGYQSLPLLPRKRREAGWRSHRVCHVPLCLPARVKQLSLSLCKSPGQPSFL
IRESfinder result
IRESfinder score
0.6674691
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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