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mmu_ciSmarcad1_521
Basic information of circular RNA
PanCircBase ID:
mmu_ciSmarcad1_521
mm39_Chr. Position ID:
chr6|65073150|65073671|+
Gene name:
Smarcad1
Transcript:
ENSMUST00000031984.9
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
15
Exon/intron offsets:
0
Genomic length:
521
Exon/intron sizes in nt:
521
Flanking exon/introns:
chr6:65073150-65073673
Splice length:
521
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGAGTGTGCTTTGTAAATAATTGTGATAAAATTTATCTTTCCTAATTTAATACATACACACATGATTTTAAATCATGAATTAGCAAGCTTTTCCTTTAAAGTTCTTTTTGCTTTTGGGGCCCTGTGGCCACTGTCATACTTTCTTCAATAATTCTTTAAACATGTAAAAAAAACAAAAACCCCAAAACCAAAAAAACCCCCATACTTTTAGTGCCCTATTGCACATGAGCTCCAGTTTGTATAAATATAGTTTATTTTTTAACCACCTCTCAGAAGTTATTTGAGGATACACAATTGTGCTTTCAAAAGATGCCAGCAGGTTCATGTTCTGCCTGAGGTATAGTAGATGATAGTAAATGACATCAAATATCTCACCTTTTATTCTATCACAGAATGTAAAATTGTAAATGATAATTGAATTATCTTGTTACCTTGTCTCCTTTACTGAGTAAGATAGACAATTAGGGAGGGAGAGGTGCTTAAAGAAGAAAACTAATGTGAGTTTTGTCTTCATTCTC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGGAGGGAGAGGTGCTTAA
59.58
AGGAAAAGCTTGCTAATTCATGA
56.75
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTAGGGAGGGAGAGGTGCTT
59.58
AGGAAAAGCTTGCTAATTCATGA
56.75
155
PCR template sequence:
ATTATCTTGTTACCTTGTCTCCTTTACTGAGTAAGATAGACAATTAGGGAGGGAGAGGTGCTTAAAGAAGAAAACTAATGTGAGTTTTGTCTTCATTCTCGTAAGAGTGTGCTTTGTAAATAATTGTGATAAAATTTATCTTTCCTAATTTAATACATACACACATGATTTTAAATCATGAATTAGCAAGCTTTTCCTTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-680(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGTCTTCATTCTCGTAAGAGTGTGCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.937922979
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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