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mmu_ciRpl7a_225
Basic information of circular RNA
PanCircBase ID:
mmu_ciRpl7a_225
mm39_Chr. Position ID:
chr2|26802457|26802682|+
Gene name:
Rpl7a
Transcript:
ENSMUST00000102898.5
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
5
Exon/intron offsets:
0
Genomic length:
225
Exon/intron sizes in nt:
225
Flanking exon/introns:
chr2:26802457-26802682
Splice length:
225
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAGGTGTGAGGTTCTGTGTGGCTTCTGGAAGGTTGGGATCTCTTGCTGGGTTTGAAGAGCTGGGGAAAAGATTGGTGTGGCTCTTGCAATGATGTCAATCTTTGACTGAAGTGACCTTGAAGTGCAATTACTGAGCTTTTTAACCCTGAGCAATTGCCACAAAGTATCTGTGCAGCTAGCTGTTGCTTCTGCCAAAACAACCTACAGCTCATGTTTGCTTCTAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCCTGAGCAATTGCCACAA
60.47
TGCAAGAGCCACACCAATCT
59.89
171
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACCCTGAGCAATTGCCACA
60.47
TGCAAGAGCCACACCAATCT
59.89
172
PCR template sequence:
CAATTACTGAGCTTTTTAACCCTGAGCAATTGCCACAAAGTATCTGTGCAGCTAGCTGTTGCTTCTGCCAAAACAACCTACAGCTCATGTTTGCTTCTAGGTAGGTGTGAGGTTCTGTGTGGCTTCTGGAAGGTTGGGATCTCTTGCTGGGTTTGAAGAGCTGGGGAAAAGATTGGTGTGGCTCTTGCAATGATGTCAAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-92a-3p(1), mmu-miR-335-5p(1), mmu-miR-25-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGTTTGCTTCTAGGTAGGTGTGAGGT
siRNA sets
CTCACACCTACCTAGAAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.756941766
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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