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mmu_circZzef1_296
Basic information of circular RNA
PanCircBase ID:
mmu_circZzef1_296
mm39_Chr. Position ID:
chr11|72721403|72722547|+
Gene name:
Zzef1
Transcript:
ENSMUST00000150551.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
7,8
Exon/intron offsets:
0,965
Genomic length:
1144
Exon/intron sizes in nt:
1,17,179
Flanking exon/introns:
chr11:72717088-72721403|chr11:72722547-72728008
Splice length:
296
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAAGGCACTGCAGCACATGCCACCGCTGTCCCTCTCTCCAGGATCTACGGACTTCTCAACTTTCCTCTCTCCTAATGTTCTGGAAGAAGTGGACAGCTTCCTCATAAGGATAACTAGCTGCTGTTCTACTCCAGAGGTAGAGCTGACTCTTCTGGCTTTTGCACTAGCAAGAGGAAGTATTGCCAAAGTAATGAGCTCTCTGTGTACCATCACTGACCACTTGGACACACAGTATGACGCCTCATCCCTCATCTCATCCATGGCATCTGTCAGGCAGAACCTGCTCCTGAAATATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGACACACAGTATGACGCC
60.04
AGCTGTCCACTTCTTCCAGA
58.28
173
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGACACACAGTATGACGCCT
59.75
AGCTGTCCACTTCTTCCAGA
58.28
172
PCR template sequence:
TCTCTGTGTACCATCACTGACCACTTGGACACACAGTATGACGCCTCATCCCTCATCTCATCCATGGCATCTGTCAGGCAGAACCTGCTCCTGAAATATGGAAGGCACTGCAGCACATGCCACCGCTGTCCCTCTCTCCAGGATCTACGGACTTCTCAACTTTCCTCTCTCCTAATGTTCTGGAAGAAGTGGACAGCTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-185-5p(2), mmu-miR-130a-3p(1), mmu-miR-130b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCCTGAAATATGGAAGGCACTGCAG
siRNA sets
AGTGCCTTCCATATTTCAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZZEF1_296_ORF_1
0.496
0.04126492
0.202106738
387
ORF sequence:
ATGCCACCGCTGTCCCTCTCTCCAGGATCTACGGACTTCTCAACTTTCCTCTCTCCTAATGTTCTGGAAGAAGTGGACAGCTTCCTCATAAGGATAACTAGCTGCTGTTCTACTCCAGAGGTAGAGCTGACTCTTCTGGCTTTTGCACTAGCAAGAGGAAGTATTGCCAAAGTAATGAGCTCTCTGTGTACCATCACTGACCACTTGGACACACAGTATGACGCCTCATCCCTCATCTCATCCATGGCATCTGTCAGGCAGAACCTGCTCCTGAAATATGGAAGGCACTGCAGCACATGCCACCGCTGTCCCTCTCTCCAGGATCTACGGACTTCTCAACTTTCCTCTCTCCTAATGTTCTGGAAGAAGTGGACAGCTTCCTCATAA
Peptide sequence:
MPPLSLSPGSTDFSTFLSPNVLEEVDSFLIRITSCCSTPEVELTLLAFALARGSIAKVMSSLCTITDHLDTQYDASSLISSMASVRQNLLLKYGRHCSTCHRCPSLQDLRTSQLSSLLMFWKKWTASS
IRESfinder result
IRESfinder score
0.490292894
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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