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mmu_circZzef1_291
Basic information of circular RNA
PanCircBase ID:
mmu_circZzef1_291
mm39_Chr. Position ID:
chr11|72784087|72786111|+
Gene name:
Zzef1
Transcript:
ENSMUST00000069395.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
38,39
Exon/intron offsets:
0,1884
Genomic length:
2024
Exon/intron sizes in nt:
151,140
Flanking exon/introns:
chr11:72782051-72784087|chr11:72786111-72790329
Splice length:
291
Other database IDs:
circBase: mmu_circ_0003341, CIRCpedia: MMU_CIRCpedia_36462, circAtlas: mmu-Zzef1_0002
circAtlas: hsa-ZZEF1_0002, CIRCpedia: HSA_CIRCpedia_20624
Human Ortholog IDs:
Mature spliced Sequence:
GGAAAGAGAGCTGCAGATACGGAAGTAAGACCCTTGGATTATAAGCAGAAGAAAAAGGCAGGTGAAGATCTCTCCATAGTGAAGGACCCTTCATGCCAGACCCAGGTTTCAGATGCACCTGCCAGTGCTCACGTGCCTCCAGGACTTCCAGATGCTGAACATCCAGAAGTGTCAGCTCAGGTACTGGTAGAAGAGAAGGCGATTACTCCAAACCCTGAGCAAGTGTTTGCAGAGTGTTCCCAGAAGAGGATTTTGGGATTGCTGGCAGCCATGTTACCTCCCATCAAGTCG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCAAACCCTGAGCAAGTGT
59.45
TGGAGAGATCTTCACCTGCC
58.8
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCAAACCCTGAGCAAGTGT
59.45
TGGAGAGATCTTCACCTGCCT
60.27
161
PCR template sequence:
AGAGAAGGCGATTACTCCAAACCCTGAGCAAGTGTTTGCAGAGTGTTCCCAGAAGAGGATTTTGGGATTGCTGGCAGCCATGTTACCTCCCATCAAGTCGGGAAAGAGAGCTGCAGATACGGAAGTAAGACCCTTGGATTATAAGCAGAAGAAAAAGGCAGGTGAAGATCTCTCCATAGTGAAGGACCCTTCATGCCAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-3470b(1), mmu-miR-3105-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCCCATCAAGTCGGGAAAGAGAGCTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZZEF1_291_ORF_1
1.0734
0.247747743
0.500961757
312
ORF sequence:
ATGTTACCTCCCATCAAGTCGGGAAAGAGAGCTGCAGATACGGAAGTAAGACCCTTGGATTATAAGCAGAAGAAAAAGGCAGGTGAAGATCTCTCCATAGTGAAGGACCCTTCATGCCAGACCCAGGTTTCAGATGCACCTGCCAGTGCTCACGTGCCTCCAGGACTTCCAGATGCTGAACATCCAGAAGTGTCAGCTCAGGTACTGGTAGAAGAGAAGGCGATTACTCCAAACCCTGAGCAAGTGTTTGCAGAGTGTTCCCAGAAGAGGATTTTGGGATTGCTGGCAGCCATGTTACCTCCCATCAAGTCG
Peptide sequence:
MLPPIKSGKRAADTEVRPLDYKQKKKAGEDLSIVKDPSCQTQVSDAPASAHVPPGLPDAEHPEVSAQVLVEEKAITPNPEQVFAECSQKRILGLLAAMLPPIKS
IRESfinder result
IRESfinder score
0.422269911
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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