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mmu_circZfr_947
Basic information of circular RNA
PanCircBase ID:
mmu_circZfr_947
mm39_Chr. Position ID:
chr15|12149613|12156494|+
Gene name:
Zfr
Transcript:
ENSMUST00000122941.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
7,8,9,10,11
Exon/intron offsets:
0,645,3273,4918,6735
Genomic length:
6881
Exon/intron sizes in nt:
192,292,197,120,146
Flanking exon/introns:
chr15:12146482-12149613|chr15:12156494-12159684
Splice length:
947
Other database IDs:
CIRCpedia: MMU_CIRCpedia_5498, circAtlas: mmu-Zfr_0027
circBase: hsa_circ_0128849, circAtlas: hsa-ZFR_0023, CIRCpedia: HSA_CIRCpedia_123387
Human Ortholog IDs:
Mature spliced Sequence:
ACTTATAAAGAGCACTTAGAAGGACAGAAACATAAAAAAAAGGAAGCCGCATTGAAAGCCTCTCAAAATACCAGCAGCAGCAACAACTCAACTCGTGGGACACAAAATCAGCTACGTTGCGAGCTCTGTGATGTGTCTTGTACAGGAGCAGATGCATATGCTGCCCACATTCGTGGTGCTAAGCATCAGAAAGTGGTTAAATTACACACAAAACTTGGTAAACCTATTCCTTCAACGGAACCAAATGTTGTTAGCCAAGCTACTTCTTCAACAGCTGCATCTGCTTCAAAACCAACTGCCTCTCCTTCAAGTATTGGCGCAAGCAATTGTACTCTGAACACGTCATCAATTGCAACTTCTTCAGTGAAAGGTCTTTCAACTACAGGAAACTCGTCTCTTAATAGTACATCCAACACTAAAGTGTCAGCGATACCTACCAATATGGCTGCCAAGAAAACATCTACACCCAAAATAAACTTTGTTGGTGGAAATAAGCTACAGTCAACAGGAAATAAAACGGAAGACTTAAAAGGAATTGACTGTGTTAAGAATACTCCTGCTGCCTCTGCTGTGCAGATTCCAGAAGTAAAGCAAGATGCAGGGTCAGAACCAGTCACACCTGCGTCCCTTGCTGCTCTGCAGAGTGATGTACAACCAGTGGGACATGATTATGTTGAAGAGGTACGAAATGATGAAGGAAAAGTCATTCGCTTCCATTGTAAATTATGTGAATGCAGCTTTAATGACCCCAATGCTAAAGAGATGCACCTAAAAGGACGAAGACATAGACTTCAGTATAAAAAAAAAGTAAATCCAGATTTGCAAGTAGAAGTGAAGCCCAGTATTCGTGCCAGAAAAATTCAAGAAGAGAAAATGAGAAAGCAAATGCAGAAGGAGGAATATTGGCGGAGACGAGAAGAGGAGGAACGCTGGAGGATGGAGATAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAGGAATATTGGCGGAGAC
59.31
AGTTGTTGCTGCTGCTGGTA
60.18
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAGGAATATTGGCGGAGAC
59.31
CCCACGAGTTGAGTTGTTGC
59.69
154
PCR template sequence:
GTGCCAGAAAAATTCAAGAAGAGAAAATGAGAAAGCAAATGCAGAAGGAGGAATATTGGCGGAGACGAGAAGAGGAGGAACGCTGGAGGATGGAGATAAGACTTATAAAGAGCACTTAGAAGGACAGAAACATAAAAAAAAGGAAGCCGCATTGAAAGCCTCTCAAAATACCAGCAGCAGCAACAACTCAACTCGTGGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(1), mmu-let-7a-5p(1), mmu-let-7b-5p(1), mmu-miR-135a-5p(1), mmu-miR-298-5p(1), mmu-miR-709(1), mmu-miR-320-3p(1), mmu-miR-138-5p(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
GGATGGAGATAAGACTTATAAAGAGC
siRNA sets
CTTTATAAGTCTTATCTCC, TTTATAAGTCTTATCTCCA, TATAAGTCTTATCTCCATC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZFR_947_ORF_1
1.0112
0.091868395
0.680763195
525
ORF sequence:
ATGGCTGCCAAGAAAACATCTACACCCAAAATAAACTTTGTTGGTGGAAATAAGCTACAGTCAACAGGAAATAAAACGGAAGACTTAAAAGGAATTGACTGTGTTAAGAATACTCCTGCTGCCTCTGCTGTGCAGATTCCAGAAGTAAAGCAAGATGCAGGGTCAGAACCAGTCACACCTGCGTCCCTTGCTGCTCTGCAGAGTGATGTACAACCAGTGGGACATGATTATGTTGAAGAGGTACGAAATGATGAAGGAAAAGTCATTCGCTTCCATTGTAAATTATGTGAATGCAGCTTTAATGACCCCAATGCTAAAGAGATGCACCTAAAAGGACGAAGACATAGACTTCAGTATAAAAAAAAAGTAAATCCAGATTTGCAAGTAGAAGTGAAGCCCAGTATTCGTGCCAGAAAAATTCAAGAAGAGAAAATGAGAAAGCAAATGCAGAAGGAGGAATATTGGCGGAGACGAGAAGAGGAGGAACGCTGGAGGATGGAGATAAGACTTATAAAGAGCACTTAG
Peptide sequence:
MAAKKTSTPKINFVGGNKLQSTGNKTEDLKGIDCVKNTPAASAVQIPEVKQDAGSEPVTPASLAALQSDVQPVGHDYVEEVRNDEGKVIRFHCKLCECSFNDPNAKEMHLKGRRHRLQYKKKVNPDLQVEVKPSIRARKIQEEKMRKQMQKEEYWRRREEEERWRMEIRLIKST
IRESfinder result
IRESfinder score
0.515158378
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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