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mmu_circZfp654_2917
Basic information of circular RNA
PanCircBase ID:
mmu_circZfp654_2917
mm39_Chr. Position ID:
chr16|64604869|64614919|-
Gene name:
Zfp654
Transcript:
ENSMUST00000207826.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
8,7,6,5,4
Exon/intron offsets:
0,3798,6580,7262,9914
Genomic length:
10050
Exon/intron sizes in nt:
2,29,61,42,14,02,03,130
Flanking exon/introns:
chr16:64603639-64604869|chr16:64614919-64634987
Splice length:
2917
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAATGCCAGAACCACCTCCGCAGATATGGAAATGTGAATCTTGAACTGGTGACTCGAATCATTAAAGATGGTGGCCCATGGGAAGACCCAGTGTTGCAAGCTGTTCTTAAAGCCCAACCGGCATCTCAGGAGATAGTGAACAAATACTTAAGTTCTGAAAATCCACTGTTCTTTGAACTACGTGCCAGATACCTAATTGCCTGTGAACGCATACCTGAAGCAATGGCTCTTATTAAATCTTGTATAAATCACCCTGAAATCAGCAAAGACTTGTACTTCCATCAAGCACTCTTCACCTGTCTGTTTATGTCACCTGTAGAAGATCAGCTATTCCGTGAGCATTTGTTGAAAACTGACTGTAAGAGTGGAATTGATATCATCTGTAACACTGAAAAAGAAGGCAAGACTTTGCTAGCCTTACAGCTCTGTGAATCCTTTCTTATTCCACAGCTCCAGAATGGGGACATGTACTACATATGGGAGTTGATTTTCCTATGGAGTAAACTACAGCTTAAATCTAATCCTTCCAAACAAGTGTTTGTAGATCAGTGTTACCAGCTTTTAAGAACAGCAACTAATGTGAGAGTCATATTTCCTTTTATGAAAATCATTAAAGATGAGGTTGAAGAAGAAGGCCTACAAATTTGTGTTGAGATCTGTGGTTGTGCTCTCCAGCTTGATCTCCATGATGATCCTGAAACTAAATGTCTAATTTATAAAACAATTGCACATTTTTTGCCAAATGATCTAGAGATTGTCAGGGTTTGTGCTCTCTCAGTATTTTTTCTTGAGCGTTCCTTAGATGCCTACCACACCGTTGAAGAGCTTTACAGACGTCCGGATGAAGAGTATAGTGAAGGCATGAGTACTGTTCAAAACCGTGTTCGTTTTGAATTACTTCCAATTTTGAAAAAGGGATTGTTTTTTGATCCTGAGTTTTGGAACTTCGTAATGATTAAGAAAAACTGTGTGGCATTACTGCGTGATAAATCAGCAGTGAAACTTCTAAATGAAAATACACTGGAAAATCCTTCAAGTAGTCTAAAAAAGAGAGTGGACCAGCAGAGTGTGGAAGAAGATCAGAGCACTGGAGAGACTGATCCTGATGATGCGTCTGTAGTGCAACCTAAAGGACAGGTTAATGTGAAGAGAAGCCTGTCTGCTCTTAACACCTCCAAAGTGGATCATAGTGTCCCAAGACATCGGTGCATGTTATGCAACAAGGAATTTCTTGGTGGCCACATTGTAAGGCATGCCCAGGCTCATCAGAAAAAAGGCAGTTTTGCATGTGTGATATGTGGTAGGAAATTTAGGAACAGAGGACTTATGCAGAAGCATTTAAAAAATCACGTTAAGAAGATTCAGAGACAGCAGATTGCTACTGCACAACAGGATGATCCAGAAGTCATCACTTTGGAAGAAATAAATGGTTCCAAATCTTTAATTTCCTTTGAAAATGGGAATTCAAATACTAAGGGCTTGGAAATAGAGACATTGACGGCTTCCAGTGAGAGAAACAAAGAGGTCATCCGTGAACACATGGCTGAGTTCATTAAAATTCCCATTGCTATACCAGAGAATGCTATAGAAAACATTATTGAAAATGGCAAACCAGATGCTTCTTTCAATAATATCTCAGAATCTTTACCTCAGTGTGATGATGACTATGAAGAGGAAGAGAATGAAGATGATTATGAAGATGATTATGATTTGAATCAAGAAACATCAGTGCTTCACAAAATCAATGGAACTGTGTGCCATCCAAAAGATGTATATGCTACAGACCAAGAAGGCAACTTCAAGTGTCCTGCTCTTGGCTGTGTAAGGATATTTAAAAGAATTGGGTTCCTAAATATGCATGCAAGGACTGTGCATCCAACTGATTTAAATGTGCGGCAAACTGTAATGAAGTGGAGTAAAGGAAAGTGCAAATTTTGCCAAAGGCAGTTTGAGGATTCTCAACATTTTATAGATCACCTTAATAGACACAGCTATCCAAATGTGTACTTCTGTTTGCATTTCAATTGCAATGAGTCATTTAAGCTGCCATTTCAGCTTGCCCAGCATACAAAAAGTCACAGGATATTTCAAGCTCAGTGTAGTTTTCCAGAATGCCATGAGCTTTTTGAAGATCTTCCTCTGTTATATGAACATGAAGCCCAGCATTACTTGAGCAAAACACCAGAATCATCTGCACAGCTGAGTGAAGTGGTTCCTAACCATCAAGAAATAGACCCATTTAGTAATGAGAACCAAACTATTCATCATCCAGTTTCTACTAGTAAGTCAAGGAAATATTCTACAGAACCAAAGACATATATAGACACTATGGAAAAGAAAACAGACAGTTTAGTTCATAATGGAAATGAACATTCTGATGATACTGTTTCAAATATAAGCTTGATAGACCAAAAGATGCCTGCCATAGAGCCAAATCCTGAAAATACTCATAGTACCACTGACTTAGTCAATGGACACAGTGAAATAGAGCAAACACCATTAGTTACATCAGATCCTACTCTGAAAATAGATATAAACAGAAACAGGACAGAAAATGGTTCTATTTTACCCAGTGTTGAATCACAAGAACACAGTGCCCTGTCAGTATCTCAGGCACCATCCAAACCAAATCTTACAAGTGAACAGACTTCATATGGCTTAATTGTAACAAAGCCATTTGTCAGACCTTTGCCCCCCAGTTACCTTGATGAACGATACCTTAGTATGCCAAAACGCAGAAAATTTCTCACCGATATTGTAGATGCCTGTTCTGATCAAGATAACATGTATAAAAAACCAGTGAAAAGATTACGATGTGGCAAATGCCTGACCACCTACTGTAATGCAGAAGCACTTGAGGCTCACCTTGCACAAAAGAAATGTCAGACACTCTTTGGATTTGATTCAGATGATGAAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGAAGCACTTGAGGCTCACC
59.96
CTTGCAACACTGGGTCTTCC
59.05
171
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGAAGCACTTGAGGCTCA
60.6
CTTGCAACACTGGGTCTTCC
59.05
173
PCR template sequence:
GGCAAATGCCTGACCACCTACTGTAATGCAGAAGCACTTGAGGCTCACCTTGCACAAAAGAAATGTCAGACACTCTTTGGATTTGATTCAGATGATGAAAGAATGCCAGAACCACCTCCGCAGATATGGAAATGTGAATCTTGAACTGGTGACTCGAATCATTAAAGATGGTGGCCCATGGGAAGACCCAGTGTTGCAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
28
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(2), mmu-miR-200a-3p(2), mmu-miR-141-3p(2), mmu-miR-23a-3p(2), mmu-miR-532-5p(2), mmu-miR-137-3p(1), mmu-miR-23b-3p(1), mmu-miR-135a-5p(1), mmu-miR-223-3p(1), mmu-miR-26a-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-203-3p(1), mmu-miR-26b-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-483-5p(1), mmu-miR-182-5p(1), mmu-miR-301b-3p(1), mmu-miR-183-5p(1), mmu-miR-301a-3p(1), mmu-miR-383-5p(1), mmu-miR-680(1), mmu-miR-377-3p(1), mmu-miR-1198-5p(1), mmu-miR-410-3p(1), mmu-miR-539-5p(1), mmu-miR-3065-5p(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
TCAGATGATGAAAGAATGCCAGAACC
siRNA sets
GTTCTGGCATTCTTTCATC, TTCTGGCATTCTTTCATCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZFP654_2917_ORF_1
1.0028
0.004267996
0.999999986
2991
ORF sequence:
ATGCAGAAGCACTTGAGGCTCACCTTGCACAAAAGAAATGTCAGACACTCTTTGGATTTGATTCAGATGATGAAAGAATGCCAGAACCACCTCCGCAGATATGGAAATGTGAATCTTGAACTGGTGACTCGAATCATTAAAGATGGTGGCCCATGGGAAGACCCAGTGTTGCAAGCTGTTCTTAAAGCCCAACCGGCATCTCAGGAGATAGTGAACAAATACTTAAGTTCTGAAAATCCACTGTTCTTTGAACTACGTGCCAGATACCTAATTGCCTGTGAACGCATACCTGAAGCAATGGCTCTTATTAAATCTTGTATAAATCACCCTGAAATCAGCAAAGACTTGTACTTCCATCAAGCACTCTTCACCTGTCTGTTTATGTCACCTGTAGAAGATCAGCTATTCCGTGAGCATTTGTTGAAAACTGACTGTAAGAGTGGAATTGATATCATCTGTAACACTGAAAAAGAAGGCAAGACTTTGCTAGCCTTACAGCTCTGTGAATCCTTTCTTATTCCACAGCTCCAGAATGGGGACATGTACTACATATGGGAGTTGATTTTCCTATGGAGTAAACTACAGCTTAAATCTAATCCTTCCAAACAAGTGTTTGTAGATCAGTGTTACCAGCTTTTAAGAACAGCAACTAATGTGAGAGTCATATTTCCTTTTATGAAAATCATTAAAGATGAGGTTGAAGAAGAAGGCCTACAAATTTGTGTTGAGATCTGTGGTTGTGCTCTCCAGCTTGATCTCCATGATGATCCTGAAACTAAATGTCTAATTTATAAAACAATTGCACATTTTTTGCCAAATGATCTAGAGATTGTCAGGGTTTGTGCTCTCTCAGTATTTTTTCTTGAGCGTTCCTTAGATGCCTACCACACCGTTGAAGAGCTTTACAGACGTCCGGATGAAGAGTATAGTGAAGGCATGAGTACTGTTCAAAACCGTGTTCGTTTTGAATTACTTCCAATTTTGAAAAAGGGATTGTTTTTTGATCCTGAGTTTTGGAACTTCGTAATGATTAAGAAAAACTGTGTGGCATTACTGCGTGATAAATCAGCAGTGAAACTTCTAAATGAAAATACACTGGAAAATCCTTCAAGTAGTCTAAAAAAGAGAGTGGACCAGCAGAGTGTGGAAGAAGATCAGAGCACTGGAGAGACTGATCCTGATGATGCGTCTGTAGTGCAACCTAAAGGACAGGTTAATGTGAAGAGAAGCCTGTCTGCTCTTAACACCTCCAAAGTGGATCATAGTGTCCCAAGACATCGGTGCATGTTATGCAACAAGGAATTTCTTGGTGGCCACATTGTAAGGCATGCCCAGGCTCATCAGAAAAAAGGCAGTTTTGCATGTGTGATATGTGGTAGGAAATTTAGGAACAGAGGACTTATGCAGAAGCATTTAAAAAATCACGTTAAGAAGATTCAGAGACAGCAGATTGCTACTGCACAACAGGATGATCCAGAAGTCATCACTTTGGAAGAAATAAATGGTTCCAAATCTTTAATTTCCTTTGAAAATGGGAATTCAAATACTAAGGGCTTGGAAATAGAGACATTGACGGCTTCCAGTGAGAGAAACAAAGAGGTCATCCGTGAACACATGGCTGAGTTCATTAAAATTCCCATTGCTATACCAGAGAATGCTATAGAAAACATTATTGAAAATGGCAAACCAGATGCTTCTTTCAATAATATCTCAGAATCTTTACCTCAGTGTGATGATGACTATGAAGAGGAAGAGAATGAAGATGATTATGAAGATGATTATGATTTGAATCAAGAAACATCAGTGCTTCACAAAATCAATGGAACTGTGTGCCATCCAAAAGATGTATATGCTACAGACCAAGAAGGCAACTTCAAGTGTCCTGCTCTTGGCTGTGTAAGGATATTTAAAAGAATTGGGTTCCTAAATATGCATGCAAGGACTGTGCATCCAACTGATTTAAATGTGCGGCAAACTGTAATGAAGTGGAGTAAAGGAAAGTGCAAATTTTGCCAAAGGCAGTTTGAGGATTCTCAACATTTTATAGATCACCTTAATAGACACAGCTATCCAAATGTGTACTTCTGTTTGCATTTCAATTGCAATGAGTCATTTAAGCTGCCATTTCAGCTTGCCCAGCATACAAAAAGTCACAGGATATTTCAAGCTCAGTGTAGTTTTCCAGAATGCCATGAGCTTTTTGAAGATCTTCCTCTGTTATATGAACATGAAGCCCAGCATTACTTGAGCAAAACACCAGAATCATCTGCACAGCTGAGTGAAGTGGTTCCTAACCATCAAGAAATAGACCCATTTAGTAATGAGAACCAAACTATTCATCATCCAGTTTCTACTAGTAAGTCAAGGAAATATTCTACAGAACCAAAGACATATATAGACACTATGGAAAAGAAAACAGACAGTTTAGTTCATAATGGAAATGAACATTCTGATGATACTGTTTCAAATATAAGCTTGATAGACCAAAAGATGCCTGCCATAGAGCCAAATCCTGAAAATACTCATAGTACCACTGACTTAGTCAATGGACACAGTGAAATAGAGCAAACACCATTAGTTACATCAGATCCTACTCTGAAAATAGATATAAACAGAAACAGGACAGAAAATGGTTCTATTTTACCCAGTGTTGAATCACAAGAACACAGTGCCCTGTCAGTATCTCAGGCACCATCCAAACCAAATCTTACAAGTGAACAGACTTCATATGGCTTAATTGTAACAAAGCCATTTGTCAGACCTTTGCCCCCCAGTTACCTTGATGAACGATACCTTAGTATGCCAAAACGCAGAAAATTTCTCACCGATATTGTAGATGCCTGTTCTGATCAAGATAACATGTATAAAAAACCAGTGAAAAGATTACGATGTGGCAAATGCCTGACCACCTACTGTAATGCAGAAGCACTTGAGGCTCACCTTGCACAAAAGAAATGTCAGACACTCTTTGGATTTGATTCAGATGATGAA
Peptide sequence:
MQKHLRLTLHKRNVRHSLDLIQMMKECQNHLRRYGNVNLELVTRIIKDGGPWEDPVLQAVLKAQPASQEIVNKYLSSENPLFFELRARYLIACERIPEAMALIKSCINHPEISKDLYFHQALFTCLFMSPVEDQLFREHLLKTDCKSGIDIICNTEKEGKTLLALQLCESFLIPQLQNGDMYYIWELIFLWSKLQLKSNPSKQVFVDQCYQLLRTATNVRVIFPFMKIIKDEVEEEGLQICVEICGCALQLDLHDDPETKCLIYKTIAHFLPNDLEIVRVCALSVFFLERSLDAYHTVEELYRRPDEEYSEGMSTVQNRVRFELLPILKKGLFFDPEFWNFVMIKKNCVALLRDKSAVKLLNENTLENPSSSLKKRVDQQSVEEDQSTGETDPDDASVVQPKGQVNVKRSLSALNTSKVDHSVPRHRCMLCNKEFLGGHIVRHAQAHQKKGSFACVICGRKFRNRGLMQKHLKNHVKKIQRQQIATAQQDDPEVITLEEINGSKSLISFENGNSNTKGLEIETLTASSERNKEVIREHMAEFIKIPIAIPENAIENIIENGKPDASFNNISESLPQCDDDYEEEENEDDYEDDYDLNQETSVLHKINGTVCHPKDVYATDQEGNFKCPALGCVRIFKRIGFLNMHARTVHPTDLNVRQTVMKWSKGKCKFCQRQFEDSQHFIDHLNRHSYPNVYFCLHFNCNESFKLPFQLAQHTKSHRIFQAQCSFPECHELFEDLPLLYEHEAQHYLSKTPESSAQLSEVVPNHQEIDPFSNENQTIHHPVSTSKSRKYSTEPKTYIDTMEKKTDSLVHNGNEHSDDTVSNISLIDQKMPAIEPNPENTHSTTDLVNGHSEIEQTPLVTSDPTLKIDINRNRTENGSILPSVESQEHSALSVSQAPSKPNLTSEQTSYGLIVTKPFVRPLPPSYLDERYLSMPKRRKFLTDIVDACSDQDNMYKKPVKRLRCGKCLTTYCNAEALEAHLAQKKCQTLFGFDSDDE
IRESfinder result
IRESfinder score
0.713653712
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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