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mmu_circZfp512_860
Basic information of circular RNA
PanCircBase ID:
mmu_circZfp512_860
mm39_Chr. Position ID:
chr5|31623575|31630305|+
Gene name:
Zfp512
Transcript:
ENSMUST00000076264.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
4,5,6,7,8,9,10
Exon/intron offsets:
0,360,1090,1922,4126,4765,6538
Genomic length:
6730
Exon/intron sizes in nt:
1,05,84,12,58,79,91,68,000
Flanking exon/introns:
chr5:31622977-31623575|chr5:31630305-31630796
Splice length:
860
Other database IDs:
CIRCpedia: MMU_CIRCpedia_16152, circAtlas: mmu-Zfp512_0003
circAtlas: hsa-RP11-158I13_0005, CIRCpedia: HSA_CIRCpedia_174143
Human Ortholog IDs:
Mature spliced Sequence:
GCGCAGGTGAAAAGGAAAAGGAGAGGGCCAAAGGAAAGAGGAACGTCAAGCAAGAAGAAGATGAAGACTATCGCGAGCTTCCTCAAAAGAAGCACAAGCTGTATGGGAGGAAGCAGCGGCCTAAAGCTCAGCCTCACCCCAAACCCCAGGCTCGTCGGGTCCGGAAGGAGCCGCCAGTCTATGCAGCAGGCAGTATGGAGGAAAAGTGGTACTTAGAGATCATGGATAAAGGCAGTGTGTCCTGTCCTACCTGCCAGGCAGTGGGGAGGAAGACCATAGAGGGTTTAAAGAAACACATGGAAAACTGCAAACAGGAAATGTTTACCTGCCATCATTGTGGGAAACAACTTCATTCACTGGCCGGAATGAAGTATCACGTCATGGCCAATCATAATAGTCTGCCAATTTTGAAGGCAGGAGATGAAGTAGATGAGCCAAGTGAGAGGGAGAGGCTGCGAACAGTTTTAAAGAGAATGGGAAAGCTCCGGTGCATGCGTGAGAGTTGCTCCAGTACCTTCACCAGCATTATGGGATATCTGTACCATGTTAGAAAGTGTGGCAAAGAGGCTTCAGAGCTCGAGAAGTTGGCCCTGAAATGTCACCACTGTGGGAAACCGTACAGGTCAAAGGCTGGGCTTGCATACCACATGAGGTCAGAGCATGGGCCTGTCTTCTTCCCAGAGTCAGAACAGCCAGACTGTTTAAAGGAGATGAGTTTGGAGGCAAAGGGTGGAGGCCGAGTTCAGCGGCGTTCTGCCAAGATAGCTGTGTACCATCTGCAGGAGCTGGCCTCCGCTGAACTAACCAAGGAATGGCCGAAGAGGAAGGTCCTCCAGGACCTGGTACCTGATGACCGGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAAGGAATGGCCGAAGAGGA
59.75
TCTTTTGAGGAAGCTCGCGA
59.68
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AATGGCCGAAGAGGAAGGTC
59.75
TCTTTTGAGGAAGCTCGCGA
59.68
140
PCR template sequence:
GATAGCTGTGTACCATCTGCAGGAGCTGGCCTCCGCTGAACTAACCAAGGAATGGCCGAAGAGGAAGGTCCTCCAGGACCTGGTACCTGATGACCGGAAGGCGCAGGTGAAAAGGAAAAGGAGAGGGCCAAAGGAAAGAGGAACGTCAAGCAAGAAGAAGATGAAGACTATCGCGAGCTTCCTCAAAAGAAGCACAAGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-30e-5p(1), mmu-miR-328-3p(1), mmu-miR-30d-5p(1), mmu-miR-127-3p(1), mmu-miR-501-3p(1), mmu-miR-324-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-300-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGATGACCGGAAGGCGCAGGTGAAAA
siRNA sets
TTTTCACCTGCGCCTTCCG,TTTCACCTGCGCCTTCCGG,TTCACCTGCGCCTTCCGGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZFP512_860_ORF_1
0.7404
0.174524094
0.959763012
882
ORF sequence:
ATGGAGGAAAAGTGGTACTTAGAGATCATGGATAAAGGCAGTGTGTCCTGTCCTACCTGCCAGGCAGTGGGGAGGAAGACCATAGAGGGTTTAAAGAAACACATGGAAAACTGCAAACAGGAAATGTTTACCTGCCATCATTGTGGGAAACAACTTCATTCACTGGCCGGAATGAAGTATCACGTCATGGCCAATCATAATAGTCTGCCAATTTTGAAGGCAGGAGATGAAGTAGATGAGCCAAGTGAGAGGGAGAGGCTGCGAACAGTTTTAAAGAGAATGGGAAAGCTCCGGTGCATGCGTGAGAGTTGCTCCAGTACCTTCACCAGCATTATGGGATATCTGTACCATGTTAGAAAGTGTGGCAAAGAGGCTTCAGAGCTCGAGAAGTTGGCCCTGAAATGTCACCACTGTGGGAAACCGTACAGGTCAAAGGCTGGGCTTGCATACCACATGAGGTCAGAGCATGGGCCTGTCTTCTTCCCAGAGTCAGAACAGCCAGACTGTTTAAAGGAGATGAGTTTGGAGGCAAAGGGTGGAGGCCGAGTTCAGCGGCGTTCTGCCAAGATAGCTGTGTACCATCTGCAGGAGCTGGCCTCCGCTGAACTAACCAAGGAATGGCCGAAGAGGAAGGTCCTCCAGGACCTGGTACCTGATGACCGGAAGGCGCAGGTGAAAAGGAAAAGGAGAGGGCCAAAGGAAAGAGGAACGTCAAGCAAGAAGAAGATGAAGACTATCGCGAGCTTCCTCAAAAGAAGCACAAGCTGTATGGGAGGAAGCAGCGGCCTAAAGCTCAGCCTCACCCCAAACCCCAGGCTCGTCGGGTCCGGAAGGAGCCGCCAGTCTATGCAGCAGGCAGTATGGAGGAAAAGTGGTACTTAG
Peptide sequence:
MEEKWYLEIMDKGSVSCPTCQAVGRKTIEGLKKHMENCKQEMFTCHHCGKQLHSLAGMKYHVMANHNSLPILKAGDEVDEPSERERLRTVLKRMGKLRCMRESCSSTFTSIMGYLYHVRKCGKEASELEKLALKCHHCGKPYRSKAGLAYHMRSEHGPVFFPESEQPDCLKEMSLEAKGGGRVQRRSAKIAVYHLQELASAELTKEWPKRKVLQDLVPDDRKAQVKRKRRGPKERGTSSKKKMKTIASFLKRSTSCMGGSSGLKLSLTPNPRLVGSGRSRQSMQQAVWRKSGT
IRESfinder result
IRESfinder score
0.332854505
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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