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mmu_circZfp354b_308
Basic information of circular RNA
PanCircBase ID:
mmu_circZfp354b_308
mm39_Chr. Position ID:
chr11|50819390|50821812|-
Gene name:
Zfp354b
Transcript:
ENSMUST00000109124.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
4,3,2
Exon/intron offsets:
0,813,2337
Genomic length:
2422
Exon/intron sizes in nt:
96,127,85
Flanking exon/introns:
chr11:50814667-50819390|chr11:50821812-50822331
Splice length:
308
Other database IDs:
CIRCpedia: HSA_CIRCpedia_354597
Human Ortholog IDs:
Mature spliced Sequence:
ATCGCATTTTTGGACACTGTACCCATCTTTGAGAAAAGCCCAGAAAGTGAGCATGGCTCCAGAGCAAAGGGAAGGGAAGTCTCAGGTGTCGGTGACGTTTGAAGACGTGGCCGTGCTCTTTACCCGGGATGAGTGGAAGAAGCTGGTTCCCTCTCAGAGAAGCCTGTACCGTGAGGTGATGCTGGAGAACTACAGCAACCTGGCCTCGCTGGGATTCCCATTTACCAAACCAAAGATGATCTCCGTGTTGCAACAAGGAGAAGAACCCTGGAAATCAGAGAAAGAAAGTCATGGATGCTCCCCTCTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCAACAAGGAGAAGAACCCT
59.51
CACCGACACCTGAGACTTCC
60.04
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCAACAAGGAGAAGAACCCT
59.51
AAACGTCACCGACACCTGAG
60.25
160
PCR template sequence:
CTGGGATTCCCATTTACCAAACCAAAGATGATCTCCGTGTTGCAACAAGGAGAAGAACCCTGGAAATCAGAGAAAGAAAGTCATGGATGCTCCCCTCTTGATCGCATTTTTGGACACTGTACCCATCTTTGAGAAAAGCCCAGAAAGTGAGCATGGCTCCAGAGCAAAGGGAAGGGAAGTCTCAGGTGTCGGTGACGTTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-204-5p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGCTCCCCTCTTGATCGCATTTTTGG
siRNA sets
AAAAATGCGATCAAGAGGG, AAAATGCGATCAAGAGGGG, AAATGCGATCAAGAGGGGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZFP354B_308_ORF_1
0.9518
0.267225948
0.421493349
288
ORF sequence:
ATGGCTCCAGAGCAAAGGGAAGGGAAGTCTCAGGTGTCGGTGACGTTTGAAGACGTGGCCGTGCTCTTTACCCGGGATGAGTGGAAGAAGCTGGTTCCCTCTCAGAGAAGCCTGTACCGTGAGGTGATGCTGGAGAACTACAGCAACCTGGCCTCGCTGGGATTCCCATTTACCAAACCAAAGATGATCTCCGTGTTGCAACAAGGAGAAGAACCCTGGAAATCAGAGAAAGAAAGTCATGGATGCTCCCCTCTTGATCGCATTTTTGGACACTGTACCCATCTTTGA
Peptide sequence:
MAPEQREGKSQVSVTFEDVAVLFTRDEWKKLVPSQRSLYREVMLENYSNLASLGFPFTKPKMISVLQQGEEPWKSEKESHGCSPLDRIFGHCTHL
IRESfinder result
IRESfinder score
0.493296524
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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