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mmu_circZfp341_382
Basic information of circular RNA
PanCircBase ID:
mmu_circZfp341_382
mm39_Chr. Position ID:
chr2|154474198|154476197|+
Gene name:
Zfp341
Transcript:
ENSMUST00000081926.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
8,9
Exon/intron offsets:
0,1808
Genomic length:
1999
Exon/intron sizes in nt:
1,91,191
Flanking exon/introns:
chr2:154472419-154474198|chr2:154476197-154479933
Splice length:
382
Other database IDs:
circAtlas: mmu-Zfp341_0001
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_95677
Human Ortholog IDs:
Mature spliced Sequence:
CCACACAGGGGAGAAGCCCTTCCAGTGCATTGCGTGTGGCCGTGCCTTTGCCCAGAAGTCTAACGTCAAGAAGCACATGCAGACTCATAAGGTGTGGCCTCCAGGGCGAAGTGGCGGCACAGTCTCCCGAAACTCTGTGACCGTACAAGTCATGGCTTTGAACCCCAACAGGCAGGAAGAAGAAGACACGGGGTTGGGTCAGTCACTGTCCAGTACCACACAGCCCCAGGCCTTGCCCACGGCAGGTGAGGATGAAGGTGACAAGCCAGAGGCCAAGCAGGTGGTCCTGATCGACAGCTCTTACCTGTGTCAGTTCTGCCCCAGCAAGTTCAGCACCTACTTCCAGCTCAAGTCCCACATGATTCAGCACAAGAAGGAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCAGCAAGTTCAGCACCTA
59.96
CTGCATGTGCTTCTTGACGT
59.13
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCAGCAAGTTCAGCACCTA
59.96
GTCTGCATGTGCTTCTTGACG
60.14
147
PCR template sequence:
GGTCCTGATCGACAGCTCTTACCTGTGTCAGTTCTGCCCCAGCAAGTTCAGCACCTACTTCCAGCTCAAGTCCCACATGATTCAGCACAAGAAGGAGCAGCCACACAGGGGAGAAGCCCTTCCAGTGCATTGCGTGTGGCCGTGCCTTTGCCCAGAAGTCTAACGTCAAGAAGCACATGCAGACTCATAAGGTGTGGCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(1), mmu-miR-205-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAAGAAGGAGCAGCCACACAGGGGAG
siRNA sets
TCCCCTGTGTGGCTGCTCC, CTCCCCTGTGTGGCTGCTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZFP341_382_ORF_1
1.1309
0.298789819
0.674046903
369
ORF sequence:
ATGCAGACTCATAAGGTGTGGCCTCCAGGGCGAAGTGGCGGCACAGTCTCCCGAAACTCTGTGACCGTACAAGTCATGGCTTTGAACCCCAACAGGCAGGAAGAAGAAGACACGGGGTTGGGTCAGTCACTGTCCAGTACCACACAGCCCCAGGCCTTGCCCACGGCAGGTGAGGATGAAGGTGACAAGCCAGAGGCCAAGCAGGTGGTCCTGATCGACAGCTCTTACCTGTGTCAGTTCTGCCCCAGCAAGTTCAGCACCTACTTCCAGCTCAAGTCCCACATGATTCAGCACAAGAAGGAGCAGCCACACAGGGGAGAAGCCCTTCCAGTGCATTGCGTGTGGCCGTGCCTTTGCCCAGAAGTCTAA
Peptide sequence:
MQTHKVWPPGRSGGTVSRNSVTVQVMALNPNRQEEEDTGLGQSLSSTTQPQALPTAGEDEGDKPEAKQVVLIDSSYLCQFCPSKFSTYFQLKSHMIQHKKEQPHRGEALPVHCVWPCLCPEV
IRESfinder result
IRESfinder score
0.491124444
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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