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mmu_circZfp326_559
Basic information of circular RNA
PanCircBase ID:
mmu_circZfp326_559
mm39_Chr. Position ID:
chr5|106038983|106055004|+
Gene name:
Zfp326
Transcript:
ENSMUST00000150440.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
6,7,8,9
Exon/intron offsets:
0,5374,14797,15921
Genomic length:
16021
Exon/intron sizes in nt:
1,99,11,21,48,100
Flanking exon/introns:
chr5:106036705-106038983|chr5:106055004-106058036
Splice length:
559
Other database IDs:
circAtlas: mmu-Zfp326_0003
circBase: hsa_circ_0114412, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_125050
Human Ortholog IDs:
Mature spliced Sequence:
CATATGGGTGATTTTGGAAGCTTCCATAGACCTGGAATTATTGTTGACTATCAAAACAAACCTGCCAATGTGACGATTGCTACTGCAAGAGGAATAAAAAGAAAAATGATGCAAATATTTATTAAGCCCGGGGGAGCCTTTATCAAGAAGCCTAAGCTAGCGAAACCTATGGATAAGATGAACCTCAGCAAATCACCTACAAAAACTGATCCTAAAAATGAAGAAGAAGAAAAGCGGCGAATTGAGGCTCGGCGAGAGAAGCAAAGACGTAGACGAGAAAAAAACAGTGAAAAATATGGAGATGGATACAGAATGGCATTCACATGTTCATTTTGTAAATTTCGAACCTTTGAAGAAAAAGATATTGAACTGCATCTGGAAAGTTCTTCTCACCAGGAAACATTAGATCATATTCAGAAACAAACCAAATTTGATAAAGTAGTTATGGAGTTTTTACATGAATGTATGGTGAATAAATTTAAAAAAGCATCTATTCGTAAGCAACAGACACTTAATCACCCAGAAGCTTACAAAATAATTGAAAAAGATATTATGGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCAACAGACACTTAATCACCCA
60.18
GCAATCGTCACATTGGCAGG
60.18
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCAACAGACACTTAATCACCC
58.59
GCAATCGTCACATTGGCAGG
60.18
140
PCR template sequence:
GAATGTATGGTGAATAAATTTAAAAAAGCATCTATTCGTAAGCAACAGACACTTAATCACCCAGAAGCTTACAAAATAATTGAAAAAGATATTATGGAAGCATATGGGTGATTTTGGAAGCTTCCATAGACCTGGAATTATTGTTGACTATCAAAACAAACCTGCCAATGTGACGATTGCTACTGCAAGAGGAATAAAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-23a-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GATATTATGGAAGCATATGGGTGATT
siRNA sets
AATCACCCATATGCTTCCA, ATCACCCATATGCTTCCAT, CATATGCTTCCATAATATC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZFP326_559_ORF_1
0.9117
0.042429605
0.66626135
567
ORF sequence:
ATGGGTGATTTTGGAAGCTTCCATAGACCTGGAATTATTGTTGACTATCAAAACAAACCTGCCAATGTGACGATTGCTACTGCAAGAGGAATAAAAAGAAAAATGATGCAAATATTTATTAAGCCCGGGGGAGCCTTTATCAAGAAGCCTAAGCTAGCGAAACCTATGGATAAGATGAACCTCAGCAAATCACCTACAAAAACTGATCCTAAAAATGAAGAAGAAGAAAAGCGGCGAATTGAGGCTCGGCGAGAGAAGCAAAGACGTAGACGAGAAAAAAACAGTGAAAAATATGGAGATGGATACAGAATGGCATTCACATGTTCATTTTGTAAATTTCGAACCTTTGAAGAAAAAGATATTGAACTGCATCTGGAAAGTTCTTCTCACCAGGAAACATTAGATCATATTCAGAAACAAACCAAATTTGATAAAGTAGTTATGGAGTTTTTACATGAATGTATGGTGAATAAATTTAAAAAAGCATCTATTCGTAAGCAACAGACACTTAATCACCCAGAAGCTTACAAAATAATTGAAAAAGATATTATGGAAGCATATGGGTGA
Peptide sequence:
MGDFGSFHRPGIIVDYQNKPANVTIATARGIKRKMMQIFIKPGGAFIKKPKLAKPMDKMNLSKSPTKTDPKNEEEEKRRIEARREKQRRRREKNSEKYGDGYRMAFTCSFCKFRTFEEKDIELHLESSSHQETLDHIQKQTKFDKVVMEFLHECMVNKFKKASIRKQQTLNHPEAYKIIEKDIMEAYG
IRESfinder result
IRESfinder score
0.629262884
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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