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mmu_circZfp326_459
Basic information of circular RNA
PanCircBase ID:
mmu_circZfp326_459
mm39_Chr. Position ID:
chr5|106038983|106053928|+
Gene name:
Zfp326
Transcript:
ENSMUST00000150440.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
6,7,8
Exon/intron offsets:
0,5374,14797
Genomic length:
14945
Exon/intron sizes in nt:
19,91,12,148
Flanking exon/introns:
chr5:106036705-106038983|chr5:106053928-106054904
Splice length:
459
Other database IDs:
circAtlas: mmu-Zfp326_0009
Human Ortholog IDs:
Mature spliced Sequence:
CATATGGGTGATTTTGGAAGCTTCCATAGACCTGGAATTATTGTTGACTATCAAAACAAACCTGCCAATGTGACGATTGCTACTGCAAGAGGAATAAAAAGAAAAATGATGCAAATATTTATTAAGCCCGGGGGAGCCTTTATCAAGAAGCCTAAGCTAGCGAAACCTATGGATAAGATGAACCTCAGCAAATCACCTACAAAAACTGATCCTAAAAATGAAGAAGAAGAAAAGCGGCGAATTGAGGCTCGGCGAGAGAAGCAAAGACGTAGACGAGAAAAAAACAGTGAAAAATATGGAGATGGATACAGAATGGCATTCACATGTTCATTTTGTAAATTTCGAACCTTTGAAGAAAAAGATATTGAACTGCATCTGGAAAGTTCTTCTCACCAGGAAACATTAGATCATATTCAGAAACAAACCAAATTTGATAAAGTAGTTATGGAGTTTTTACAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAAAGTTCTTCTCACCAGGA
58.95
GCAATCGTCACATTGGCAGG
60.18
163
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGGAAAGTTCTTCTCACCAGGA
59.93
GCAATCGTCACATTGGCAGG
60.18
164
PCR template sequence:
AGATATTGAACTGCATCTGGAAAGTTCTTCTCACCAGGAAACATTAGATCATATTCAGAAACAAACCAAATTTGATAAAGTAGTTATGGAGTTTTTACATCATATGGGTGATTTTGGAAGCTTCCATAGACCTGGAATTATTGTTGACTATCAAAACAAACCTGCCAATGTGACGATTGCTACTGCAAGAGGAATAAAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-23a-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGAGTTTTTACATCATATGGGTGATT
siRNA sets
AATCACCCATATGATGTAA, CATATGATGTAAAAACTCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZFP326_459_ORF_1
0.916
0.081543152
0.966920189
915
ORF sequence:
ATGGGTGATTTTGGAAGCTTCCATAGACCTGGAATTATTGTTGACTATCAAAACAAACCTGCCAATGTGACGATTGCTACTGCAAGAGGAATAAAAAGAAAAATGATGCAAATATTTATTAAGCCCGGGGGAGCCTTTATCAAGAAGCCTAAGCTAGCGAAACCTATGGATAAGATGAACCTCAGCAAATCACCTACAAAAACTGATCCTAAAAATGAAGAAGAAGAAAAGCGGCGAATTGAGGCTCGGCGAGAGAAGCAAAGACGTAGACGAGAAAAAAACAGTGAAAAATATGGAGATGGATACAGAATGGCATTCACATGTTCATTTTGTAAATTTCGAACCTTTGAAGAAAAAGATATTGAACTGCATCTGGAAAGTTCTTCTCACCAGGAAACATTAGATCATATTCAGAAACAAACCAAATTTGATAAAGTAGTTATGGAGTTTTTACATCATATGGGTGATTTTGGAAGCTTCCATAGACCTGGAATTATTGTTGACTATCAAAACAAACCTGCCAATGTGACGATTGCTACTGCAAGAGGAATAAAAAGAAAAATGATGCAAATATTTATTAAGCCCGGGGGAGCCTTTATCAAGAAGCCTAAGCTAGCGAAACCTATGGATAAGATGAACCTCAGCAAATCACCTACAAAAACTGATCCTAAAAATGAAGAAGAAGAAAAGCGGCGAATTGAGGCTCGGCGAGAGAAGCAAAGACGTAGACGAGAAAAAAACAGTGAAAAATATGGAGATGGATACAGAATGGCATTCACATGTTCATTTTGTAAATTTCGAACCTTTGAAGAAAAAGATATTGAACTGCATCTGGAAAGTTCTTCTCACCAGGAAACATTAGATCATATTCAGAAACAAACCAAATTTGATAAAGTAGTTATGGAGTTTTTACAT
Peptide sequence:
MGDFGSFHRPGIIVDYQNKPANVTIATARGIKRKMMQIFIKPGGAFIKKPKLAKPMDKMNLSKSPTKTDPKNEEEEKRRIEARREKQRRRREKNSEKYGDGYRMAFTCSFCKFRTFEEKDIELHLESSSHQETLDHIQKQTKFDKVVMEFLHHMGDFGSFHRPGIIVDYQNKPANVTIATARGIKRKMMQIFIKPGGAFIKKPKLAKPMDKMNLSKSPTKTDPKNEEEEKRRIEARREKQRRRREKNSEKYGDGYRMAFTCSFCKFRTFEEKDIELHLESSSHQETLDHIQKQTKFDKVVMEFLH
IRESfinder result
IRESfinder score
0.596150223
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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