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mmu_circZc3h7a_298
Basic information of circular RNA
PanCircBase ID:
mmu_circZc3h7a_298
mm39_Chr. Position ID:
chr16|10956884|10958714|-
Gene name:
Zc3h7a
Transcript:
ENSMUST00000037633.16
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
22,21
Exon/intron offsets:
0,1696
Genomic length:
1830
Exon/intron sizes in nt:
164,134
Flanking exon/introns:
chr16:10955349-10956884|chr16:10958714-10959526
Splice length:
298
Other database IDs:
CIRCpedia: MMU_CIRCpedia_221824, circAtlas: mmu-Zc3h7a_0008
circAtlas: hsa-ZC3H7A_0025
Human Ortholog IDs:
Mature spliced Sequence:
TACATGATATAGAGCAATTTTATGAACTCTGGCTGAAGAACCAAAAAACTGAAAAAAGTGATGAGACAGCCAGTCACTGTAGCAAAGAAAACGGGAAGCAGATCCATATGCCAACAGACTATGCTGAAGTCACTGTGGACTTCCACTGCTGGATGTGTGGGAAGAACTGTAACAGTGAGAAGCAATGGCAAGACCACATCTCCTCTGAGAAGCACAAGGAGAAGGTTTTCCACACTGAGGATGACCAGTACTGCTGGCAGCACCGCTTCCCCACTGGGTACTTCAGTATTTGTGAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCACACTGAGGATGACCAGT
59.02
TGCTACAGTGACTGGCTGTC
59.68
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCACACTGAGGATGACCAGT
59.02
GCTACAGTGACTGGCTGTCT
59.39
152
PCR template sequence:
TCTCCTCTGAGAAGCACAAGGAGAAGGTTTTCCACACTGAGGATGACCAGTACTGCTGGCAGCACCGCTTCCCCACTGGGTACTTCAGTATTTGTGAGAGTACATGATATAGAGCAATTTTATGAACTCTGGCTGAAGAACCAAAAAACTGAAAAAAGTGATGAGACAGCCAGTCACTGTAGCAAAGAAAACGGGAAGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(1), mmu-miR-298-5p(1), mmu-miR-139-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
GTATTTGTGAGAGTACATGATATAGA
siRNA sets
CTATATCATGTACTCTCAC, TATATCATGTACTCTCACA, TCTATATCATGTACTCTCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZC3H7A_298_ORF_1
0.7171
0.092072396
0.576016605
534
ORF sequence:
ATGAGACAGCCAGTCACTGTAGCAAAGAAAACGGGAAGCAGATCCATATGCCAACAGACTATGCTGAAGTCACTGTGGACTTCCACTGCTGGATGTGTGGGAAGAACTGTAACAGTGAGAAGCAATGGCAAGACCACATCTCCTCTGAGAAGCACAAGGAGAAGGTTTTCCACACTGAGGATGACCAGTACTGCTGGCAGCACCGCTTCCCCACTGGGTACTTCAGTATTTGTGAGAGTACATGATATAGAGCAATTTTATGAACTCTGGCTGAAGAACCAAAAAACTGAAAAAAGTGATGAGACAGCCAGTCACTGTAGCAAAGAAAACGGGAAGCAGATCCATATGCCAACAGACTATGCTGAAGTCACTGTGGACTTCCACTGCTGGATGTGTGGGAAGAACTGTAACAGTGAGAAGCAATGGCAAGACCACATCTCCTCTGAGAAGCACAAGGAGAAGGTTTTCCACACTGAGGATGACCAGTACTGCTGGCAGCACCGCTTCCCCACTGGGTACTTCAGTATTTGTGAG
Peptide sequence:
MRQPVTVAKKTGSRSICQQTMLKSLWTSTAGCVGRTVTVRSNGKTTSPLRSTRRRFSTLRMTSTAGSTASPLGTSVFVRVHDIEQFYELWLKNQKTEKSDETASHCSKENGKQIHMPTDYAEVTVDFHCWMCGKNCNSEKQWQDHISSEKHKEKVFHTEDDQYCWQHRFPTGYFSICE
IRESfinder result
IRESfinder score
0.318110428
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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