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mmu_circZc3h7a_1745
Basic information of circular RNA
PanCircBase ID:
mmu_circZc3h7a_1745
mm39_Chr. Position ID:
chr16|10963509|10976627|-
Gene name:
Zc3h7a
Transcript:
ENSMUST00000037633.16
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
13
Exon/intron indexes:
18,17,16,15,14,13,12,11,10,9,8,7,6
Exon/intron offsets:
0,721,1623,2799,3565,4949,5493,6220,7413,10374,10882,12908,13037
Genomic length:
13118
Exon/intron sizes in nt:
134,77,182,101,197,162,187,65,205,281,34,39,81
Flanking exon/introns:
chr16:10963149-10963509|chr16:10976627-10976829
Splice length:
1745
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GATGAACATGTAATAAAATTAACTCAAGAATTAGCTCAGAAATTGGGATTTAAAATAAGGAAAGCATATGTTAGAGCTGAGCACTTACTGAAAGCAGTTCCTGGGGATGGGGCTCCCAAGGCTGTGAACTATTCTGTGGAAGATATTGAGCCAGATTTGTTAGCCCCAAGGCAAGAAGCACTTTCGGTTGCCTCCCTTCCTACATCCAGTTTTTCTCATGTCATTGGGAGTGAGCTGGCCTCTGTTCCTATTATGCCGGTAACTTCTGTTTTGCAACTGCAAGTGGATGAGAGTCCTCTGCCATCCACAGTGTTTGCAAATGGAGGGAAGATCCCCTTCACCATATCCGATGCTTTTCTGGATGATGACATTGTCCTTGGGGATGAAATAGATGCTCTTCTTGATTCTGCCCCAGAAACAAATGATGCTGTTATGTCCTCCACTCTGGTCAGAGGACCCCTCCCGACTGCCAGTGCAGCTCCGAGCTTGCCCTTCAGGACACAATCCCTGCTTGGAACCTTGCCCATGGGTACAAGGTATCCTGAGCCCTCCTTTGCAGACTTTTTTCCACCTCTGACTTCATCCTTGGAAGAATTTTACTCTTCTTTAAATTCATTTTCAGTGACTGAATCCAAACGAGATCTGTGCACCTCAACTGCTAGAGAGGGAACACCGCTTAACAACAGTAATTCCTCCCTTTTACTTATGAATGGACCAGGTAATTTGTTGGCCTCTGAAAATTTTCTGGGAATTACAAGTCAGCCTAGAAATGACTTTGGAAACTTTTTTGGAAGTGTGATCGCTAAACCATTTTCATCAGGGACTCCTAGGCATCCCCTCGAAGGAACCCACGAGCTGAGGCAAGCTTGCCAGATCTGCTTTGTAAAATCAGGCCCTAAGTTAATGGATTTCACATACCATGCTAATGTAGATCATAAATGTAAGAAAGATATTTTAATTGGTAGGATAAAAAATGTTGAAGATAAATCATGGAAAAAAATACGTCCAAGACCAACAAAAACAAATTATGAAGGACCATATTATATATGCAAAGATGTTGCTGCAGATGATGAATGCCGATATCCTGGCCACTGTACATTTGCTTATTGCCAAGAAGAGATAGATGTGTGGACCCTGGAAAGAAAAGGGGCATTCAGTCGAGAGGCTTTCTTTGGGGGCAATGGAAAGATCAACCTTACTGTGGTCAGAATTCTCCAAGAGCATCATGGAGAGTTTACATTCCTTTGTGAGAAATGTTTTGATCATAAACCTAGAATGATAAGTAAAAGAAATAAAGATAATTCCACTGCCTGCTCTCACCCCGTCACAAAGCATGAATTTGAGGACAATAAGTGCCTTGTCCACATTTTGAGAGAGACCACAGTAAAATATTCCAAAATACGTTCTTTTCATAAACAATGTCAATTTGATATTTGTCGACATGAAGTTCGTTATGGCTGCGTGAGGGAAGATGAGTGCTTCTATGCCCATAGTCTTATTGAACTGAAAGTCTGGATAATGCAAAGTGAAAGAGGTATCTCCCATGATGATATTGCTCAACAATCCAAACAATATTGGAGAAGTTTGCAAGCAAGCATGCCGGGAGTGCAGGTCCTTGGTAATCAAATAATGCCTGGATTACTTAACATGGAGATAAAGTTTGTATGTGCTCAGTGTCTGAGAAATGGCCAAGTCATTGAACCAGACAGGAACAGAAAGTACTGCAGTGCCAAAGCACAGCATTC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCCAAGTCATTGAACCAGA
59.86
TGCTTTCAGTAAGTGCTCAGC
58.85
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCCAAGTCATTGAACCAGA
59.86
CTGCTTTCAGTAAGTGCTCAGC
59.84
157
PCR template sequence:
ACATGGAGATAAAGTTTGTATGTGCTCAGTGTCTGAGAAATGGCCAAGTCATTGAACCAGACAGGAACAGAAAGTACTGCAGTGCCAAAGCACAGCATTCGATGAACATGTAATAAAATTAACTCAAGAATTAGCTCAGAAATTGGGATTTAAAATAAGGAAAGCATATGTTAGAGCTGAGCACTTACTGAAAGCAGTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
22
Interacting miRNAs (#binding sites)
mmu-miR-541-5p(2), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-1a-3p(1), mmu-miR-206-3p(1), mmu-miR-34c-5p(1), mmu-miR-298-5p(1), mmu-miR-320-3p(1), mmu-miR-324-5p(1), mmu-miR-149-5p(1), mmu-miR-136-5p(1), mmu-miR-96-5p(1), mmu-miR-182-5p(1), mmu-miR-34b-5p(1), mmu-miR-384-3p(1), mmu-miR-673-5p(1), mmu-miR-5101(1), mmu-miR-3065-5p(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AAGCACAGCATTCGATGAACATGTAA
siRNA sets
TTACATGTTCATCGAATGC, TACATGTTCATCGAATGCT, TGTTCATCGAATGCTGTGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZC3H7A_1745_ORF_1
0.7188
-0.035251314
0.99881366
1506
ORF sequence:
ATGCCGGTAACTTCTGTTTTGCAACTGCAAGTGGATGAGAGTCCTCTGCCATCCACAGTGTTTGCAAATGGAGGGAAGATCCCCTTCACCATATCCGATGCTTTTCTGGATGATGACATTGTCCTTGGGGATGAAATAGATGCTCTTCTTGATTCTGCCCCAGAAACAAATGATGCTGTTATGTCCTCCACTCTGGTCAGAGGACCCCTCCCGACTGCCAGTGCAGCTCCGAGCTTGCCCTTCAGGACACAATCCCTGCTTGGAACCTTGCCCATGGGTACAAGGTATCCTGAGCCCTCCTTTGCAGACTTTTTTCCACCTCTGACTTCATCCTTGGAAGAATTTTACTCTTCTTTAAATTCATTTTCAGTGACTGAATCCAAACGAGATCTGTGCACCTCAACTGCTAGAGAGGGAACACCGCTTAACAACAGTAATTCCTCCCTTTTACTTATGAATGGACCAGGTAATTTGTTGGCCTCTGAAAATTTTCTGGGAATTACAAGTCAGCCTAGAAATGACTTTGGAAACTTTTTTGGAAGTGTGATCGCTAAACCATTTTCATCAGGGACTCCTAGGCATCCCCTCGAAGGAACCCACGAGCTGAGGCAAGCTTGCCAGATCTGCTTTGTAAAATCAGGCCCTAAGTTAATGGATTTCACATACCATGCTAATGTAGATCATAAATGTAAGAAAGATATTTTAATTGGTAGGATAAAAAATGTTGAAGATAAATCATGGAAAAAAATACGTCCAAGACCAACAAAAACAAATTATGAAGGACCATATTATATATGCAAAGATGTTGCTGCAGATGATGAATGCCGATATCCTGGCCACTGTACATTTGCTTATTGCCAAGAAGAGATAGATGTGTGGACCCTGGAAAGAAAAGGGGCATTCAGTCGAGAGGCTTTCTTTGGGGGCAATGGAAAGATCAACCTTACTGTGGTCAGAATTCTCCAAGAGCATCATGGAGAGTTTACATTCCTTTGTGAGAAATGTTTTGATCATAAACCTAGAATGATAAGTAAAAGAAATAAAGATAATTCCACTGCCTGCTCTCACCCCGTCACAAAGCATGAATTTGAGGACAATAAGTGCCTTGTCCACATTTTGAGAGAGACCACAGTAAAATATTCCAAAATACGTTCTTTTCATAAACAATGTCAATTTGATATTTGTCGACATGAAGTTCGTTATGGCTGCGTGAGGGAAGATGAGTGCTTCTATGCCCATAGTCTTATTGAACTGAAAGTCTGGATAATGCAAAGTGAAAGAGGTATCTCCCATGATGATATTGCTCAACAATCCAAACAATATTGGAGAAGTTTGCAAGCAAGCATGCCGGGAGTGCAGGTCCTTGGTAATCAAATAATGCCTGGATTACTTAACATGGAGATAAAGTTTGTATGTGCTCAGTGTCTGAGAAATGGCCAAGTCATTGAACCAGACAGGAACAGAAAGTACTGCAGTGCCAAAGCACAGCATTCGATGAACATGTAA
Peptide sequence:
MPVTSVLQLQVDESPLPSTVFANGGKIPFTISDAFLDDDIVLGDEIDALLDSAPETNDAVMSSTLVRGPLPTASAAPSLPFRTQSLLGTLPMGTRYPEPSFADFFPPLTSSLEEFYSSLNSFSVTESKRDLCTSTAREGTPLNNSNSSLLLMNGPGNLLASENFLGITSQPRNDFGNFFGSVIAKPFSSGTPRHPLEGTHELRQACQICFVKSGPKLMDFTYHANVDHKCKKDILIGRIKNVEDKSWKKIRPRPTKTNYEGPYYICKDVAADDECRYPGHCTFAYCQEEIDVWTLERKGAFSREAFFGGNGKINLTVVRILQEHHGEFTFLCEKCFDHKPRMISKRNKDNSTACSHPVTKHEFEDNKCLVHILRETTVKYSKIRSFHKQCQFDICRHEVRYGCVREDECFYAHSLIELKVWIMQSERGISHDDIAQQSKQYWRSLQASMPGVQVLGNQIMPGLLNMEIKFVCAQCLRNGQVIEPDRNRKYCSAKAQHSMNM
IRESfinder result
IRESfinder score
0.640961394
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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