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mmu_circZbtb8os_320
Basic information of circular RNA
PanCircBase ID:
mmu_circZbtb8os_320
mm39_Chr. Position ID:
chr4|129234520|129238041|+
Gene name:
Zbtb8os
Transcript:
ENSMUST00000141235.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
2,3,4,5,6
Exon/intron offsets:
0,749,1013,2393,3484
Genomic length:
3521
Exon/intron sizes in nt:
25,122,83,53,37
Flanking exon/introns:
chr4:129229932-129234520|chr4:129238041-129240596
Splice length:
320
Other database IDs:
circBase: hsa_circ_0011424, CIRCpedia: HSA_CIRCpedia_31437
Human Ortholog IDs:
Mature spliced Sequence:
ATTTGGATCATACGGCTGATGTCCAGTTACACGCATGGGGAGACACTCTGGAGGAAGCATTTGAACAGTGTGCCATGGCCATGTTTGGTTACATGACAGATACTGGGACTGTGGAGCCCCTCCGCACCGTGGAAGTAGAAACCCAAGGGGACGACCTGCAGTCTCTGCTGTTTCACTTTTTGGACGAGTGGCTTTACAAGTTCAGCGCTGATGAGTACTTCATACCCCGGGAAGTGAAAGTACTTAATATTGATCAGAAAAATTTCAAGTTACGATCCATTGGGTGGGGAGAAGAGTTCTCACTGTCCAAGCACCCTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCCCGGGAAGTGAAAGTAC
59.31
TGTCTCCCCATGCGTGTAAC
60.04
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCCCGGGAAGTGAAAGTAC
59.31
GTGTCTCCCCATGCGTGTAA
60.04
143
PCR template sequence:
CATACCCCGGGAAGTGAAAGTACTTAATATTGATCAGAAAAATTTCAAGTTACGATCCATTGGGTGGGGAGAAGAGTTCTCACTGTCCAAGCACCCTCAGATTTGGATCATACGGCTGATGTCCAGTTACACGCATGGGGAGACACTCTGGAGGAAGCATTTGAACAGTGTGCCATGGCCATGTTTGGTTACATGACAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-183-5p(1), mmu-miR-207(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAAGCACCCTCAGATTTGGATCATAC
siRNA sets
TATGATCCAAATCTGAGGG, GTATGATCCAAATCTGAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZBTB8OS_320_ORF_1
1.012
0.150294891
0.427112295
342
ORF sequence:
ATGGCCATGTTTGGTTACATGACAGATACTGGGACTGTGGAGCCCCTCCGCACCGTGGAAGTAGAAACCCAAGGGGACGACCTGCAGTCTCTGCTGTTTCACTTTTTGGACGAGTGGCTTTACAAGTTCAGCGCTGATGAGTACTTCATACCCCGGGAAGTGAAAGTACTTAATATTGATCAGAAAAATTTCAAGTTACGATCCATTGGGTGGGGAGAAGAGTTCTCACTGTCCAAGCACCCTCAGATTTGGATCATACGGCTGATGTCCAGTTACACGCATGGGGAGACACTCTGGAGGAAGCATTTGAACAGTGTGCCATGGCCATGTTTGGTTACATGA
Peptide sequence:
MAMFGYMTDTGTVEPLRTVEVETQGDDLQSLLFHFLDEWLYKFSADEYFIPREVKVLNIDQKNFKLRSIGWGEEFSLSKHPQIWIIRLMSSYTHGETLWRKHLNSVPWPCLVT
IRESfinder result
IRESfinder score
0.55230663
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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