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mmu_circZbtb20_93

Basic information of circular RNA
PanCircBase ID:

mmu_circZbtb20_93

mm39_Chr. Position ID:

chr16|42892786|42967306|+

Gene name:

Zbtb20

Transcript:

ENSMUST00000151290.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

2

Exon/intron indexes:

4,5

Exon/intron offsets:

0,74473

Genomic length:

74520

Exon/intron sizes in nt:

46,47

Flanking exon/introns:

chr16:42845350-42892786|chr16:42967306-43013827

Splice length:

93

Other database IDs:

circAtlas: mmu-Zbtb20_0015

Human Ortholog IDs:
Mature spliced Sequence:

AGTTCTTGATAAATTGCAAGTCTCTTTGTGCCTCATAGCTGCACAGGAATGGCTGTTTGATATTGGAAAACCAGTTGGTGTGCTCTTCCTTGG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

CCAGTTGGTGTGCTCTTCCT

59.89

TGCAGCTATGAGGCACAAAGA

60

66

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TGGTGTGCTCTTCCTTGGAG

59.6

TGCAGCTATGAGGCACAAAGA

60

61

PCR template sequence:

AATGGCTGTTTGATATTGGAAAACCAGTTGGTGTGCTCTTCCTTGGAGTTCTTGATAAATTGCAAGTCTCTTTGTGCCTCATAGCTGCACAG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

NA

Interacting miRNAs (#binding sites)

NA

siRNAs for circular RNA silencing
Junction sequence

TGCTCTTCCTTGGAGTTCTTGATAAA

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.791559113

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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Institute of Life sciences, Bhubaneswar, India

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