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mmu_circZbtb20_303
Basic information of circular RNA
PanCircBase ID:
mmu_circZbtb20_303
mm39_Chr. Position ID:
chr16|42967259|43283349|+
Gene name:
Zbtb20
Transcript:
ENSMUST00000151244.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
3,4,5,6,7
Exon/intron offsets:
0,46568,155672,311853,316050
Genomic length:
316090
Exon/intron sizes in nt:
47,74,47,95,40
Flanking exon/introns:
chr16:42845350-42967259|chr16:43283349-43390043
Splice length:
303
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAATGGCTGTTTGATATTGGAAAACCAGTTGGTGTGCTCTTCCTTGGACTGCCTGAAGTTACATTTTGAGACTGAAATCACTGCACCTTAAAAACAAAAGATTGGGCTGCACTGTGTTCCTACTCTTTTATCATCAGCAATTGAATATTTCACACAACTTGCTGGCTGCAAGCAAGAGGAGCTTGGAACGGAGATCGTCTGAATTCCCCAGCTCTGTCATCAGCGCTGTGACACACTCTCATTTTCTGATTCATCTGTGATGATCCCTTGACCACCAGTCTGTTGCCTGTTGCTGCTCTTCAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCTGTCATCAGCGCTGTGA
60.11
GTCCAAGGAAGAGCACACCA
59.89
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCTGTCATCAGCGCTGTGA
60.11
GCAGTCCAAGGAAGAGCACA
60.25
144
PCR template sequence:
TTCCCCAGCTCTGTCATCAGCGCTGTGACACACTCTCATTTTCTGATTCATCTGTGATGATCCCTTGACCACCAGTCTGTTGCCTGTTGCTGCTCTTCATGAATGGCTGTTTGATATTGGAAAACCAGTTGGTGTGCTCTTCCTTGGACTGCCTGAAGTTACATTTTGAGACTGAAATCACTGCACCTTAAAAACAAAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-18a-5p(1), mmu-miR-214-3p(1), mmu-miR-128-3p(1), mmu-miR-382-5p(1), mmu-miR-383-5p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGCTGCTCTTCATGAATGGCTGTTTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZBTB20_303_ORF_1
0.9421
-0.099816828
0.037604772
93
ORF sequence:
ATGAATGGCTGTTTGATATTGGAAAACCAGTTGGTGTGCTCTTCCTTGGACTGCCTGAAGTTACATTTTGAGACTGAAATCACTGCACCTTAA
Peptide sequence:
MNGCLILENQLVCSSLDCLKLHFETEITAP
IRESfinder result
IRESfinder score
0.804052294
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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