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mmu_circZbtb11_1481
Basic information of circular RNA
PanCircBase ID:
mmu_circZbtb11_1481
mm39_Chr. Position ID:
chr16|55800957|55811981|+
Gene name:
Zbtb11
Transcript:
ENSMUST00000050248.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
2,3,4,5
Exon/intron offsets:
0,1580,9664,10847
Genomic length:
11024
Exon/intron sizes in nt:
236,232,836,177
Flanking exon/introns:
chr16:55794734-55800957|chr16:55811981-55818368
Splice length:
1481
Other database IDs:
circBase: mmu_circ_0006340, CIRCpedia: MMU_CIRCpedia_23400, circAtlas: mmu-Zbtb11_0002
circBase: hsa_circ_0121367, circAtlas: hsa-ZBTB11_0002, CIRCpedia: HSA_CIRCpedia_42872
Human Ortholog IDs:
Mature spliced Sequence:
GTATATTAAAGCAAGTTAAAGATTACATAAAACAGTGTAGCAAATGTCAGGAGAAGCTCGATCGCTCCCGTCCAATATCGGATGCTTCGGAAATGTTGGAAGAGTTGGGGTTGGATCTTGACTCTGGAGAAGAAAGTAATGAGTCGGAGGATGACCTGAGCAACTTCACCTCCCCTCCATCCACAGCCTCCAAGTCTTCCAAAAAGAAGCCAGTGTCGAAACATGAGCTGGTGTTTGTTGACACCAAAGGAGTGGTCAAACGGTCCTCTCCAAAGCATTGCCAGGCGGTTCTGAAACAGTTGAATGAGCAGAGGCTTTCCAACCAGTTCTGTGACGTCACATTGCTCATCGAGGGGGAGGAGTACAAAGCGCACAAGTCTGTCCTCTCGGCTAATAGTGAATATTTTCGAGATCTTTTTATTGAAAAAGGAGCAGTTTCTAGTCACGAGGCTGTTGTGGATCTTTCTGGTTTCTGTAAAGCAAGCTTCCTTCCTTTGCTGGAATTTGCCTATACTTCTGTACTAAGTTTTGACTTTTGTAGCATGGCTGATGTAGCCGTCTTAGCTCGCCACCTTTTTATGTCAGAAGTTTTAGAAATTTGTGAAAGTGTACATAAACTAATGGAAGAAAAACAGCTTACAGTGTATAAGAAAGGAGAAGTTCAAACGGTTGCATCTACTCAGGACTTAGCAGCACACAATGGAACTACAACACCACCTGGCACTAGGAATGAAGCAACCACAACTTTGTCCGGTGAACTTGGGCATTGTGAAATTGTACTGCTGGTAAATGGAGAATTGCCAGAGGCAGAGCAGAATGGGGAGCCAGAGCAGCAGCCTGCACCTCAGGCTTCTCCAGAGGCTGAAGCTAGTGTGTCCCCTGTAGAAGGTATACCTGAGCCCCACCCTGAAATGGGAACTGCAAGCTTAGCAAAAGAAAGCAACCAACCAGAGTCCGCAGTCACCAGAGAAGATGGCATAGTTGCTAGTGTTCATCCTAAAATCTCAAAAGAGAATGTTACCAACGCCTCCCAAGAGGACAGTGATACAGGAAACGATACATCACCTGAGGACATCGGTGCCAAAGACTGTCCAGATCATAGTCAGAGCCCTGGCCAACCTTCAAAGGATGAGGACACTCTAACTGAAGCTACAGAAAAGACAGACTCAGGCCCAGATGATGATACTTATAGGAGCAGGCTTAGGCAGCGGTCTGTTAATGAAGGGGGCTATATCCGGCTACACAAGGGCATGGAGAAAAAGTTACAGAAGCGGAAAGCCATCTCTAAGTCAGCAGTTCAACAGGTGGCCCAGAAATTAGTTCAAAGAGGAAAAAAGATGAAACAACCAAAACGGGATGCTAAGGAGAGCACCGAAGAAACGGCTCATAAATGTGGTGAATGTGGAATGGTTTTTCCGAGACGATATGCCTTTATAATGCACACATTGAAACATGAAAGAGCTAGAGATTACAAATGTCCG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTGGAATGGTTTTTCCGAGACG
60.1
ATATTGGACGGGAGCGATCG
59.76
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTGGAATGGTTTTTCCGAGACG
60.1
CGATATTGGACGGGAGCGAT
59.76
160
PCR template sequence:
GGCTCATAAATGTGGTGAATGTGGAATGGTTTTTCCGAGACGATATGCCTTTATAATGCACACATTGAAACATGAAAGAGCTAGAGATTACAAATGTCCGGTATATTAAAGCAAGTTAAAGATTACATAAAACAGTGTAGCAAATGTCAGGAGAAGCTCGATCGCTCCCGTCCAATATCGGATGCTTCGGAAATGTTGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
21
Interacting miRNAs (#binding sites)
mmu-miR-207(2), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-7b-5p(1), mmu-miR-181a-5p(1), mmu-miR-221-3p(1), mmu-miR-135a-5p(1), mmu-miR-205-5p(1), mmu-miR-7a-5p(1), mmu-miR-222-3p(1), mmu-miR-181b-5p(1), mmu-miR-495-3p(1), mmu-miR-149-5p(1), mmu-miR-382-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-669d-5p(1), mmu-miR-3065-5p(1), mmu-miR-350-3p(1), mmu-miR-1904(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTACAAATGTCCGGTATATTAAAGCA
siRNA sets
TTAATATACCGGACATTTG, AATATACCGGACATTTGTA, TAATATACCGGACATTTGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZBTB11_1481_ORF_1
1.0827
0.161880426
0.999696817
1488
ORF sequence:
ATGTCCGGTATATTAAAGCAAGTTAAAGATTACATAAAACAGTGTAGCAAATGTCAGGAGAAGCTCGATCGCTCCCGTCCAATATCGGATGCTTCGGAAATGTTGGAAGAGTTGGGGTTGGATCTTGACTCTGGAGAAGAAAGTAATGAGTCGGAGGATGACCTGAGCAACTTCACCTCCCCTCCATCCACAGCCTCCAAGTCTTCCAAAAAGAAGCCAGTGTCGAAACATGAGCTGGTGTTTGTTGACACCAAAGGAGTGGTCAAACGGTCCTCTCCAAAGCATTGCCAGGCGGTTCTGAAACAGTTGAATGAGCAGAGGCTTTCCAACCAGTTCTGTGACGTCACATTGCTCATCGAGGGGGAGGAGTACAAAGCGCACAAGTCTGTCCTCTCGGCTAATAGTGAATATTTTCGAGATCTTTTTATTGAAAAAGGAGCAGTTTCTAGTCACGAGGCTGTTGTGGATCTTTCTGGTTTCTGTAAAGCAAGCTTCCTTCCTTTGCTGGAATTTGCCTATACTTCTGTACTAAGTTTTGACTTTTGTAGCATGGCTGATGTAGCCGTCTTAGCTCGCCACCTTTTTATGTCAGAAGTTTTAGAAATTTGTGAAAGTGTACATAAACTAATGGAAGAAAAACAGCTTACAGTGTATAAGAAAGGAGAAGTTCAAACGGTTGCATCTACTCAGGACTTAGCAGCACACAATGGAACTACAACACCACCTGGCACTAGGAATGAAGCAACCACAACTTTGTCCGGTGAACTTGGGCATTGTGAAATTGTACTGCTGGTAAATGGAGAATTGCCAGAGGCAGAGCAGAATGGGGAGCCAGAGCAGCAGCCTGCACCTCAGGCTTCTCCAGAGGCTGAAGCTAGTGTGTCCCCTGTAGAAGGTATACCTGAGCCCCACCCTGAAATGGGAACTGCAAGCTTAGCAAAAGAAAGCAACCAACCAGAGTCCGCAGTCACCAGAGAAGATGGCATAGTTGCTAGTGTTCATCCTAAAATCTCAAAAGAGAATGTTACCAACGCCTCCCAAGAGGACAGTGATACAGGAAACGATACATCACCTGAGGACATCGGTGCCAAAGACTGTCCAGATCATAGTCAGAGCCCTGGCCAACCTTCAAAGGATGAGGACACTCTAACTGAAGCTACAGAAAAGACAGACTCAGGCCCAGATGATGATACTTATAGGAGCAGGCTTAGGCAGCGGTCTGTTAATGAAGGGGGCTATATCCGGCTACACAAGGGCATGGAGAAAAAGTTACAGAAGCGGAAAGCCATCTCTAAGTCAGCAGTTCAACAGGTGGCCCAGAAATTAGTTCAAAGAGGAAAAAAGATGAAACAACCAAAACGGGATGCTAAGGAGAGCACCGAAGAAACGGCTCATAAATGTGGTGAATGTGGAATGGTTTTTCCGAGACGATATGCCTTTATAATGCACACATTGAAACATGAAAGAGCTAGAGATTACAAATGTCCG
Peptide sequence:
MSGILKQVKDYIKQCSKCQEKLDRSRPISDASEMLEELGLDLDSGEESNESEDDLSNFTSPPSTASKSSKKKPVSKHELVFVDTKGVVKRSSPKHCQAVLKQLNEQRLSNQFCDVTLLIEGEEYKAHKSVLSANSEYFRDLFIEKGAVSSHEAVVDLSGFCKASFLPLLEFAYTSVLSFDFCSMADVAVLARHLFMSEVLEICESVHKLMEEKQLTVYKKGEVQTVASTQDLAAHNGTTTPPGTRNEATTTLSGELGHCEIVLLVNGELPEAEQNGEPEQQPAPQASPEAEASVSPVEGIPEPHPEMGTASLAKESNQPESAVTREDGIVASVHPKISKENVTNASQEDSDTGNDTSPEDIGAKDCPDHSQSPGQPSKDEDTLTEATEKTDSGPDDDTYRSRLRQRSVNEGGYIRLHKGMEKKLQKRKAISKSAVQQVAQKLVQRGKKMKQPKRDAKESTEETAHKCGECGMVFPRRYAFIMHTLKHERARDYKCP
IRESfinder result
IRESfinder score
0.440964444
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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