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mmu_circZbtb11_1404
Basic information of circular RNA
PanCircBase ID:
mmu_circZbtb11_1404
mm39_Chr. Position ID:
chr16|55810621|55821086|+
Gene name:
Zbtb11
Transcript:
ENSMUST00000050248.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
4,5,6,7
Exon/intron offsets:
0,1183,7747,10320
Genomic length:
10465
Exon/intron sizes in nt:
8,36,17,72,46,145
Flanking exon/introns:
chr16:55802769-55810621|chr16:55821086-55823071
Splice length:
1404
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTTCTGTAAAGCAAGCTTCCTTCCTTTGCTGGAATTTGCCTATACTTCTGTACTAAGTTTTGACTTTTGTAGCATGGCTGATGTAGCCGTCTTAGCTCGCCACCTTTTTATGTCAGAAGTTTTAGAAATTTGTGAAAGTGTACATAAACTAATGGAAGAAAAACAGCTTACAGTGTATAAGAAAGGAGAAGTTCAAACGGTTGCATCTACTCAGGACTTAGCAGCACACAATGGAACTACAACACCACCTGGCACTAGGAATGAAGCAACCACAACTTTGTCCGGTGAACTTGGGCATTGTGAAATTGTACTGCTGGTAAATGGAGAATTGCCAGAGGCAGAGCAGAATGGGGAGCCAGAGCAGCAGCCTGCACCTCAGGCTTCTCCAGAGGCTGAAGCTAGTGTGTCCCCTGTAGAAGGTATACCTGAGCCCCACCCTGAAATGGGAACTGCAAGCTTAGCAAAAGAAAGCAACCAACCAGAGTCCGCAGTCACCAGAGAAGATGGCATAGTTGCTAGTGTTCATCCTAAAATCTCAAAAGAGAATGTTACCAACGCCTCCCAAGAGGACAGTGATACAGGAAACGATACATCACCTGAGGACATCGGTGCCAAAGACTGTCCAGATCATAGTCAGAGCCCTGGCCAACCTTCAAAGGATGAGGACACTCTAACTGAAGCTACAGAAAAGACAGACTCAGGCCCAGATGATGATACTTATAGGAGCAGGCTTAGGCAGCGGTCTGTTAATGAAGGGGGCTATATCCGGCTACACAAGGGCATGGAGAAAAAGTTACAGAAGCGGAAAGCCATCTCTAAGTCAGCAGTTCAACAGGTGGCCCAGAAATTAGTTCAAAGAGGAAAAAAGATGAAACAACCAAAACGGGATGCTAAGGAGAGCACCGAAGAAACGGCTCATAAATGTGGTGAATGTGGAATGGTTTTTCCGAGACGATATGCCTTTATAATGCACACATTGAAACATGAAAGAGCTAGAGATTACAAATGTCCGTTATGTAAGAAACAGTTTCAGTATAGTGCCTCATTACGAGCACACCTTATTCGACATACAAGAAAAGAGGCACCCACTTCATCCTCATCCAATTCCACATCTACTGAGGCGTCGGGAGGATCATCTGAGAAGGGAAGAACCAAAAGAGAATTTATATGTTCTATATGTGGAAGGACATTACCTAAATTATACTCTCTCCGCATACATATGCTAAAGCACACGGGTGTAAAGCCGCATGCATGCCAGGTGTGTGGAAAAACTTTTATCTATAAGCATGGTCTAAAATTGCATCAAAGTCTGCATCAATCACAAAAGCAGTTTCAGTGTGAACTGTGTGTCAAGTCATTTGTTACCAAGCGGAGTCTTCAGGAACATATGAGTATTCACACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCAAGCGGAGTCTTCAGGA
60.83
CGAGCTAAGACGGCTACATCA
59.67
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TACCAAGCGGAGTCTTCAGG
59.1
CGAGCTAAGACGGCTACATCA
59.67
141
PCR template sequence:
CAAAGTCTGCATCAATCACAAAAGCAGTTTCAGTGTGAACTGTGTGTCAAGTCATTTGTTACCAAGCGGAGTCTTCAGGAACATATGAGTATTCACACAGGTTTCTGTAAAGCAAGCTTCCTTCCTTTGCTGGAATTTGCCTATACTTCTGTACTAAGTTTTGACTTTTGTAGCATGGCTGATGTAGCCGTCTTAGCTCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
17
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-181a-5p(1), mmu-miR-221-3p(1), mmu-miR-135a-5p(1), mmu-miR-205-5p(1), mmu-miR-222-3p(1), mmu-miR-181b-5p(1), mmu-miR-149-5p(1), mmu-miR-382-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-1198-5p(1), mmu-miR-669d-5p(1), mmu-miR-350-3p(1), mmu-miR-1904(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGTATTCACACAGGTTTCTGTAAAGC
siRNA sets
AGAAACCTGTGTGAATACT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZBTB11_1404_ORF_1
1.0354
0.051677221
0.999999935
2733
ORF sequence:
ATGGCTGATGTAGCCGTCTTAGCTCGCCACCTTTTTATGTCAGAAGTTTTAGAAATTTGTGAAAGTGTACATAAACTAATGGAAGAAAAACAGCTTACAGTGTATAAGAAAGGAGAAGTTCAAACGGTTGCATCTACTCAGGACTTAGCAGCACACAATGGAACTACAACACCACCTGGCACTAGGAATGAAGCAACCACAACTTTGTCCGGTGAACTTGGGCATTGTGAAATTGTACTGCTGGTAAATGGAGAATTGCCAGAGGCAGAGCAGAATGGGGAGCCAGAGCAGCAGCCTGCACCTCAGGCTTCTCCAGAGGCTGAAGCTAGTGTGTCCCCTGTAGAAGGTATACCTGAGCCCCACCCTGAAATGGGAACTGCAAGCTTAGCAAAAGAAAGCAACCAACCAGAGTCCGCAGTCACCAGAGAAGATGGCATAGTTGCTAGTGTTCATCCTAAAATCTCAAAAGAGAATGTTACCAACGCCTCCCAAGAGGACAGTGATACAGGAAACGATACATCACCTGAGGACATCGGTGCCAAAGACTGTCCAGATCATAGTCAGAGCCCTGGCCAACCTTCAAAGGATGAGGACACTCTAACTGAAGCTACAGAAAAGACAGACTCAGGCCCAGATGATGATACTTATAGGAGCAGGCTTAGGCAGCGGTCTGTTAATGAAGGGGGCTATATCCGGCTACACAAGGGCATGGAGAAAAAGTTACAGAAGCGGAAAGCCATCTCTAAGTCAGCAGTTCAACAGGTGGCCCAGAAATTAGTTCAAAGAGGAAAAAAGATGAAACAACCAAAACGGGATGCTAAGGAGAGCACCGAAGAAACGGCTCATAAATGTGGTGAATGTGGAATGGTTTTTCCGAGACGATATGCCTTTATAATGCACACATTGAAACATGAAAGAGCTAGAGATTACAAATGTCCGTTATGTAAGAAACAGTTTCAGTATAGTGCCTCATTACGAGCACACCTTATTCGACATACAAGAAAAGAGGCACCCACTTCATCCTCATCCAATTCCACATCTACTGAGGCGTCGGGAGGATCATCTGAGAAGGGAAGAACCAAAAGAGAATTTATATGTTCTATATGTGGAAGGACATTACCTAAATTATACTCTCTCCGCATACATATGCTAAAGCACACGGGTGTAAAGCCGCATGCATGCCAGGTGTGTGGAAAAACTTTTATCTATAAGCATGGTCTAAAATTGCATCAAAGTCTGCATCAATCACAAAAGCAGTTTCAGTGTGAACTGTGTGTCAAGTCATTTGTTACCAAGCGGAGTCTTCAGGAACATATGAGTATTCACACAGGTTTCTGTAAAGCAAGCTTCCTTCCTTTGCTGGAATTTGCCTATACTTCTGTACTAAGTTTTGACTTTTGTAGCATGGCTGATGTAGCCGTCTTAGCTCGCCACCTTTTTATGTCAGAAGTTTTAGAAATTTGTGAAAGTGTACATAAACTAATGGAAGAAAAACAGCTTACAGTGTATAAGAAAGGAGAAGTTCAAACGGTTGCATCTACTCAGGACTTAGCAGCACACAATGGAACTACAACACCACCTGGCACTAGGAATGAAGCAACCACAACTTTGTCCGGTGAACTTGGGCATTGTGAAATTGTACTGCTGGTAAATGGAGAATTGCCAGAGGCAGAGCAGAATGGGGAGCCAGAGCAGCAGCCTGCACCTCAGGCTTCTCCAGAGGCTGAAGCTAGTGTGTCCCCTGTAGAAGGTATACCTGAGCCCCACCCTGAAATGGGAACTGCAAGCTTAGCAAAAGAAAGCAACCAACCAGAGTCCGCAGTCACCAGAGAAGATGGCATAGTTGCTAGTGTTCATCCTAAAATCTCAAAAGAGAATGTTACCAACGCCTCCCAAGAGGACAGTGATACAGGAAACGATACATCACCTGAGGACATCGGTGCCAAAGACTGTCCAGATCATAGTCAGAGCCCTGGCCAACCTTCAAAGGATGAGGACACTCTAACTGAAGCTACAGAAAAGACAGACTCAGGCCCAGATGATGATACTTATAGGAGCAGGCTTAGGCAGCGGTCTGTTAATGAAGGGGGCTATATCCGGCTACACAAGGGCATGGAGAAAAAGTTACAGAAGCGGAAAGCCATCTCTAAGTCAGCAGTTCAACAGGTGGCCCAGAAATTAGTTCAAAGAGGAAAAAAGATGAAACAACCAAAACGGGATGCTAAGGAGAGCACCGAAGAAACGGCTCATAAATGTGGTGAATGTGGAATGGTTTTTCCGAGACGATATGCCTTTATAATGCACACATTGAAACATGAAAGAGCTAGAGATTACAAATGTCCGTTATGTAAGAAACAGTTTCAGTATAGTGCCTCATTACGAGCACACCTTATTCGACATACAAGAAAAGAGGCACCCACTTCATCCTCATCCAATTCCACATCTACTGAGGCGTCGGGAGGATCATCTGAGAAGGGAAGAACCAAAAGAGAATTTATATGTTCTATATGTGGAAGGACATTACCTAAATTATACTCTCTCCGCATACATATGCTAAAGCACACGGGTGTAAAGCCGCATGCATGCCAGGTGTGTGGAAAAACTTTTATCTATAAGCATGGTCTAAAATTGCATCAAAGTCTGCATCAATCACAAAAGCAGTTTCAGTGTGAACTGTGTGTCAAGTCATTTGTTACCAAGCGGAGTCTTCAGGAACATATGAGTATTCACACA
Peptide sequence:
MADVAVLARHLFMSEVLEICESVHKLMEEKQLTVYKKGEVQTVASTQDLAAHNGTTTPPGTRNEATTTLSGELGHCEIVLLVNGELPEAEQNGEPEQQPAPQASPEAEASVSPVEGIPEPHPEMGTASLAKESNQPESAVTREDGIVASVHPKISKENVTNASQEDSDTGNDTSPEDIGAKDCPDHSQSPGQPSKDEDTLTEATEKTDSGPDDDTYRSRLRQRSVNEGGYIRLHKGMEKKLQKRKAISKSAVQQVAQKLVQRGKKMKQPKRDAKESTEETAHKCGECGMVFPRRYAFIMHTLKHERARDYKCPLCKKQFQYSASLRAHLIRHTRKEAPTSSSSNSTSTEASGGSSEKGRTKREFICSICGRTLPKLYSLRIHMLKHTGVKPHACQVCGKTFIYKHGLKLHQSLHQSQKQFQCELCVKSFVTKRSLQEHMSIHTGFCKASFLPLLEFAYTSVLSFDFCSMADVAVLARHLFMSEVLEICESVHKLMEEKQLTVYKKGEVQTVASTQDLAAHNGTTTPPGTRNEATTTLSGELGHCEIVLLVNGELPEAEQNGEPEQQPAPQASPEAEASVSPVEGIPEPHPEMGTASLAKESNQPESAVTREDGIVASVHPKISKENVTNASQEDSDTGNDTSPEDIGAKDCPDHSQSPGQPSKDEDTLTEATEKTDSGPDDDTYRSRLRQRSVNEGGYIRLHKGMEKKLQKRKAISKSAVQQVAQKLVQRGKKMKQPKRDAKESTEETAHKCGECGMVFPRRYAFIMHTLKHERARDYKCPLCKKQFQYSASLRAHLIRHTRKEAPTSSSSNSTSTEASGGSSEKGRTKREFICSICGRTLPKLYSLRIHMLKHTGVKPHACQVCGKTFIYKHGLKLHQSLHQSQKQFQCELCVKSFVTKRSLQEHMSIHT
IRESfinder result
IRESfinder score
0.497531272
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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