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mmu_circWipi1_392
Basic information of circular RNA
PanCircBase ID:
mmu_circWipi1_392
mm39_Chr. Position ID:
chr11|109468400|109470617|-
Gene name:
Wipi1
Transcript:
ENSMUST00000103060.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
11,10,9
Exon/intron offsets:
0,716,2052
Genomic length:
2217
Exon/intron sizes in nt:
11,91,08,165
Flanking exon/introns:
chr11:109467983-109468400|chr11:109470617-109473197
Splice length:
392
Other database IDs:
circBase: hsa_circ_0045496
Human Ortholog IDs:
Mature spliced Sequence:
CCGCCCAGAAGAGCCTTCCACCTGGAGCGGCTACATGGGAAAGATGTTCATGGCAGCTACCAACTACCTCCCCGCCCAGGTGTCGGACATGATGAACCAGGACAGGGCTTTCGCCACAGGACGCCTGAACTTCTCTGGGCAGAAGAACATTTGCACCCTGTCCACGATCCAGAAACTGCCGCGGTTGCTGGTGGCCTCCTCCGACGGACACCTTTACATCTACAACTTGGACCCACAGGATGGAGGAGAATGTGTCCTAATCAAAACCCACAGCTTGCTTAGCTCAGGAACAACAGAAGAGAACAAAGAAAATGACCTCAGACCTTCCTTACCTCCATCTTATGCTGCAACTGTAGCAAGGCCCAGCACGTCTGCAGCCTCCACGGTGCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAACTGTAGCAAGGCCCAG
61.59
CATCATGTCCGACACCTGGG
60.46
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGCAACTGTAGCAAGGCCC
61.59
CATCATGTCCGACACCTGGG
60.46
142
PCR template sequence:
ACAGAAGAGAACAAAGAAAATGACCTCAGACCTTCCTTACCTCCATCTTATGCTGCAACTGTAGCAAGGCCCAGCACGTCTGCAGCCTCCACGGTGCCAGCCGCCCAGAAGAGCCTTCCACCTGGAGCGGCTACATGGGAAAGATGTTCATGGCAGCTACCAACTACCTCCCCGCCCAGGTGTCGGACATGATGAACCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(1), mmu-miR-103-3p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-196a-5p(1), mmu-miR-339-5p(1), mmu-let-7d-5p(1), mmu-miR-22-3p(1), mmu-miR-107-3p(1), mmu-miR-98-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCCACGGTGCCAGCCGCCCAGAAGAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWIPI1_392_ORF_1
0.8165
0.1936915
0.57351479
450
ORF sequence:
ATGGGAAAGATGTTCATGGCAGCTACCAACTACCTCCCCGCCCAGGTGTCGGACATGATGAACCAGGACAGGGCTTTCGCCACAGGACGCCTGAACTTCTCTGGGCAGAAGAACATTTGCACCCTGTCCACGATCCAGAAACTGCCGCGGTTGCTGGTGGCCTCCTCCGACGGACACCTTTACATCTACAACTTGGACCCACAGGATGGAGGAGAATGTGTCCTAATCAAAACCCACAGCTTGCTTAGCTCAGGAACAACAGAAGAGAACAAAGAAAATGACCTCAGACCTTCCTTACCTCCATCTTATGCTGCAACTGTAGCAAGGCCCAGCACGTCTGCAGCCTCCACGGTGCCAGCCGCCCAGAAGAGCCTTCCACCTGGAGCGGCTACATGGGAAAGATGTTCATGGCAGCTACCAACTACCTCCCCGCCCAGGTGTCGGACATGA
Peptide sequence:
MGKMFMAATNYLPAQVSDMMNQDRAFATGRLNFSGQKNICTLSTIQKLPRLLVASSDGHLYIYNLDPQDGGECVLIKTHSLLSSGTTEENKENDLRPSLPPSYAATVARPSTSAASTVPAAQKSLPPGAATWERCSWQLPTTSPPRCRT
IRESfinder result
IRESfinder score
0.378146816
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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