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mmu_circWdr92_349
Basic information of circular RNA
PanCircBase ID:
mmu_circWdr92_349
mm39_Chr. Position ID:
chr11|17172196|17177276|+
Gene name:
Wdr92
Transcript:
ENSMUST00000151431.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
8,9,10
Exon/intron offsets:
0,2352,4964
Genomic length:
5080
Exon/intron sizes in nt:
13,11,02,116
Flanking exon/introns:
chr11:17169114-17172196|chr11:17177276-17178140
Splice length:
349
Other database IDs:
circAtlas: mmu-Wdr92_0008
Human Ortholog IDs:
Mature spliced Sequence:
GAACTTGGAAGCTCCAGAGATGCCAGTGTATTCTGTAAAGGGCCACAAAGAAATCATAAATACCATAGATGGTGTAGGTGGACTTGGAATTGGGGAAGGAGCCCCTGAAATCGTGACGGGCAGCCGTGATGGAACTGTAAAGGTGTGGGACCCAAGGCAAAAAGAGGATCCTGTCGCTAACATGGAACCTGCACAAGGAGAAAACAAGAGAGACTGTTGGACCGTGGCATTTGGCAATGCTTATAATCAAGAAGAACGTGTTGTCTGTGCTGGCTATGACAATGGGGACATCAAGCTGTTTGACCTCAGGAACATGTCACTGCGGTGGGAGACAAACATTAAAAATGGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTGGCTATGACAATGGGG
60.11
TCCCCAATTCCAAGTCCACC
59.59
178
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTGGCTATGACAATGGGG
60.11
CCCCAATTCCAAGTCCACCT
59.59
177
PCR template sequence:
AGAAGAACGTGTTGTCTGTGCTGGCTATGACAATGGGGACATCAAGCTGTTTGACCTCAGGAACATGTCACTGCGGTGGGAGACAAACATTAAAAATGGGGAACTTGGAAGCTCCAGAGATGCCAGTGTATTCTGTAAAGGGCCACAAAGAAATCATAAATACCATAGATGGTGTAGGTGGACTTGGAATTGGGGAAGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-212-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CATTAAAAATGGGGAACTTGGAAGCT
siRNA sets
AAGTTCCCCATTTTTAATG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWDR92_349_ORF_1
0.8586
0.132755264
0.553288787
462
ORF sequence:
ATGCTTATAATCAAGAAGAACGTGTTGTCTGTGCTGGCTATGACAATGGGGACATCAAGCTGTTTGACCTCAGGAACATGTCACTGCGGTGGGAGACAAACATTAAAAATGGGGAACTTGGAAGCTCCAGAGATGCCAGTGTATTCTGTAAAGGGCCACAAAGAAATCATAAATACCATAGATGGTGTAGGTGGACTTGGAATTGGGGAAGGAGCCCCTGAAATCGTGACGGGCAGCCGTGATGGAACTGTAAAGGTGTGGGACCCAAGGCAAAAAGAGGATCCTGTCGCTAACATGGAACCTGCACAAGGAGAAAACAAGAGAGACTGTTGGACCGTGGCATTTGGCAATGCTTATAATCAAGAAGAACGTGTTGTCTGTGCTGGCTATGACAATGGGGACATCAAGCTGTTTGACCTCAGGAACATGTCACTGCGGTGGGAGACAAACATTAAAAATGGG
Peptide sequence:
MLIIKKNVLSVLAMTMGTSSCLTSGTCHCGGRQTLKMGNLEAPEMPVYSVKGHKEIINTIDGVGGLGIGEGAPEIVTGSRDGTVKVWDPRQKEDPVANMEPAQGENKRDCWTVAFGNAYNQEERVVCAGYDNGDIKLFDLRNMSLRWETNIKNG
IRESfinder result
IRESfinder score
0.284163908
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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