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mmu_circWdr92_326
Basic information of circular RNA
PanCircBase ID:
mmu_circWdr92_326
mm39_Chr. Position ID:
chr11|17177160|17179866|+
Gene name:
Wdr92
Transcript:
ENSMUST00000151431.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
10,11,12
Exon/intron offsets:
0,980,2608
Genomic length:
2706
Exon/intron sizes in nt:
116,112,98
Flanking exon/introns:
chr11:17174650-17177160|chr11:17179866-17182706
Splice length:
326
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GCAATGCTTATAATCAAGAAGAACGTGTTGTCTGTGCTGGCTATGACAATGGGGACATCAAGCTGTTTGACCTCAGGAACATGTCACTGCGGTGGGAGACAAACATTAAAAATGGGGTATGCAGCTTGGAATTTGACAGAAAGGACATCAGTATGAATAAGTTGGTAGCTACATCTCTAGAAGGGAAGTTCCACGTCTTCGACATGAGAACACAGCATCCTACCAAGGGCCCATAAATCCACTGTATGGCAAGTCCGGCACCTGCCTCAAAACAGGGAGATCTTTCTGACAACGGGAGGCGCTGGGAGTCTTCACCTCTGGAAGTA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCACTGTATGGCAAGTCCGG
60.74
CCCCATTGTCATAGCCAGCA
60.11
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCACTGTATGGCAAGTCCGG
60.74
TCCCCATTGTCATAGCCAGC
59.82
143
PCR template sequence:
GGGCCCATAAATCCACTGTATGGCAAGTCCGGCACCTGCCTCAAAACAGGGAGATCTTTCTGACAACGGGAGGCGCTGGGAGTCTTCACCTCTGGAAGTAGCAATGCTTATAATCAAGAAGAACGTGTTGTCTGTGCTGGCTATGACAATGGGGACATCAAGCTGTTTGACCTCAGGAACATGTCACTGCGGTGGGAGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-709(1)
siRNAs for circular RNA silencing
Junction sequence
ACCTCTGGAAGTAGCAATGCTTATAA
siRNA sets
TATAAGCATTGCTACTTCC, ATAAGCATTGCTACTTCCA, TTATAAGCATTGCTACTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWDR92_326_ORF_1
0.7407
-0.093941104
0.072108375
237
ORF sequence:
ATGGCAAGTCCGGCACCTGCCTCAAAACAGGGAGATCTTTCTGACAACGGGAGGCGCTGGGAGTCTTCACCTCTGGAAGTAGCAATGCTTATAATCAAGAAGAACGTGTTGTCTGTGCTGGCTATGACAATGGGGACATCAAGCTGTTTGACCTCAGGAACATGTCACTGCGGTGGGAGACAAACATTAAAAATGGGGTATGCAGCTTGGAATTTGACAGAAAGGACATCAGTATGA
Peptide sequence:
MASPAPASKQGDLSDNGRRWESSPLEVAMLIIKKNVLSVLAMTMGTSSCLTSGTCHCGGRQTLKMGYAAWNLTERTSV
IRESfinder result
IRESfinder score
0.395610912
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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