top of page
mmu_circWdr7_518
Basic information of circular RNA
PanCircBase ID:
mmu_circWdr7_518
mm39_Chr. Position ID:
chr18|63860689|63868795|+
Gene name:
Wdr7
Transcript:
ENSMUST00000072726.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
5,6,7,8
Exon/intron offsets:
0,820,2471,7960
Genomic length:
8106
Exon/intron sizes in nt:
17,57,71,20,146
Flanking exon/introns:
chr18:63857270-63860689|chr18:63868795-63869474
Splice length:
518
Other database IDs:
circBase: mmu_circ_0007437, CIRCpedia: MMU_CIRCpedia_25640, circAtlas: mmu-Wdr7_0040
Human Ortholog IDs:
Mature spliced Sequence:
TTCTACCAGTTCTCTGTTGGGAACCAGCAAGAGGGCAGGCTCCTCTGCCATGGACATTACCCTGAAATCCTCGTTGTGGATGCCACCAGCCTTGAGGTGTTGTATTCCTTGGTATCGAAGATCTCTCCAGACTGGATCAGCTCCATGAGCATCATCCGCTCTCAGCGGACACAAGAGGACACTGTGGTGGCGCTCTCTGTGACAGGTATTCTGAAGGTGTGGATTGTCACCTCTGAAATGAGTGGAATGCAGGATACTGAGCCAATATTTGAGGAGGAATCCAAACCAATTTATTGTCAGAATTGCCAAAGCATCTCTTTTTGTGCATTCACACAGAGGTCACTTTTGGTCGTATGCTCCAAATACTGGAGGGTGTTTGATGCTGGCGACTACTCTCTGTTGTGCTCAGGTCCTAGTGAAAATGGACAGACATGGACTGGAGGGGACTTTGTGTCTGCAGACAAAGTCATCATCTGGACAGAAAATGGGCAGAGTTACATCTACAAACTCCCTGCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGACTGGAGGGGACTTTGTG
59.96
AATGTCCATGGCAGAGGAGC
60.11
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACATGGACTGGAGGGGACTT
60.18
AATGTCCATGGCAGAGGAGC
60.11
147
PCR template sequence:
AAAATGGACAGACATGGACTGGAGGGGACTTTGTGTCTGCAGACAAAGTCATCATCTGGACAGAAAATGGGCAGAGTTACATCTACAAACTCCCTGCCAGTTCTACCAGTTCTCTGTTGGGAACCAGCAAGAGGGCAGGCTCCTCTGCCATGGACATTACCCTGAAATCCTCGTTGTGGATGCCACCAGCCTTGAGGTGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-298-5p(1), mmu-miR-24-3p(1), mmu-miR-149-5p(1), mmu-miR-411-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AACTCCCTGCCAGTTCTACCAGTTCT
siRNA sets
AGAACTGGTAGAACTGGCA, TGGTAGAACTGGCAGGGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWDR7_518_ORF_1
0.6407
0.102326769
0.666840609
603
ORF sequence:
ATGGACTGGAGGGGACTTTGTGTCTGCAGACAAAGTCATCATCTGGACAGAAAATGGGCAGAGTTACATCTACAAACTCCCTGCCAGTTCTACCAGTTCTCTGTTGGGAACCAGCAAGAGGGCAGGCTCCTCTGCCATGGACATTACCCTGAAATCCTCGTTGTGGATGCCACCAGCCTTGAGGTGTTGTATTCCTTGGTATCGAAGATCTCTCCAGACTGGATCAGCTCCATGAGCATCATCCGCTCTCAGCGGACACAAGAGGACACTGTGGTGGCGCTCTCTGTGACAGGTATTCTGAAGGTGTGGATTGTCACCTCTGAAATGAGTGGAATGCAGGATACTGAGCCAATATTTGAGGAGGAATCCAAACCAATTTATTGTCAGAATTGCCAAAGCATCTCTTTTTGTGCATTCACACAGAGGTCACTTTTGGTCGTATGCTCCAAATACTGGAGGGTGTTTGATGCTGGCGACTACTCTCTGTTGTGCTCAGGTCCTAGTGAAAATGGACAGACATGGACTGGAGGGGACTTTGTGTCTGCAGACAAAGTCATCATCTGGACAGAAAATGGGCAGAGTTACATCTACAAACTCCCTGCC
Peptide sequence:
MDWRGLCVCRQSHHLDRKWAELHLQTPCQFYQFSVGNQQEGRLLCHGHYPEILVVDATSLEVLYSLVSKISPDWISSMSIIRSQRTQEDTVVALSVTGILKVWIVTSEMSGMQDTEPIFEEESKPIYCQNCQSISFCAFTQRSLLVVCSKYWRVFDAGDYSLLCSGPSENGQTWTGGDFVSADKVIIWTENGQSYIYKLPA
IRESfinder result
IRESfinder score
0.703637321
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page