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mmu_circWdr7_391
Basic information of circular RNA
PanCircBase ID:
mmu_circWdr7_391
mm39_Chr. Position ID:
chr18|63871813|63872424|+
Gene name:
Wdr7
Transcript:
ENSMUST00000072726.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
10,11
Exon/intron offsets:
0,362
Genomic length:
611
Exon/intron sizes in nt:
1,42,249
Flanking exon/introns:
chr18:63869577-63871813|chr18:63872424-63873097
Splice length:
391
Other database IDs:
CIRCpedia: HSA_CIRCpedia_173965
Human Ortholog IDs:
Mature spliced Sequence:
TTGGTAATTTGTCCTCCTGTTACTCGGTTTTTCTACGGATGCAAGGAATATTTGCATAAGCTACTAATTCAGGGCGATTCTTCTGGAAGGTTAAATATCTGGAACATAGCAGACATAGCAGAGAAACAGGAAGCCGATGAAGGGCTAAAGATGACAACTTGTATTAGCTTGCAAGAGGCATTTGACAAGCTGAAGCCTTGCCCTGCTGGAATTATCGATCAGCTAAGTGTGATTCCCAACAGCAACGAACCTCTTAAAGTAACTGCGAGTGTCTACATACCAGCACACGGGCGACTTGTTTGTGGCCGGGAAGATGGAAGCATCATTATTGTGCCTGCCACCCAGACGGCCATAGTTCAGCTATTGCAAGGAGAACACATGCTCAGAAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATTATTGTGCCTGCCACCCA
59.96
ACCTTCCAGAAGAATCGCCC
59.75
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGACGGCCATAGTTCAGCT
59.82
ACCTTCCAGAAGAATCGCCC
59.75
140
PCR template sequence:
CGACTTGTTTGTGGCCGGGAAGATGGAAGCATCATTATTGTGCCTGCCACCCAGACGGCCATAGTTCAGCTATTGCAAGGAGAACACATGCTCAGAAGAGTTGGTAATTTGTCCTCCTGTTACTCGGTTTTTCTACGGATGCAAGGAATATTTGCATAAGCTACTAATTCAGGGCGATTCTTCTGGAAGGTTAAATATCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-223-3p(1), mmu-miR-1198-5p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATGCTCAGAAGAGTTGGTAATTTGTC
siRNA sets
CAAATTACCAACTCTTCTG, AAATTACCAACTCTTCTGA, ACAAATTACCAACTCTTCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWDR7_391_ORF_1
0.8322
0.010630371
0.181614642
300
ORF sequence:
ATGACAACTTGTATTAGCTTGCAAGAGGCATTTGACAAGCTGAAGCCTTGCCCTGCTGGAATTATCGATCAGCTAAGTGTGATTCCCAACAGCAACGAACCTCTTAAAGTAACTGCGAGTGTCTACATACCAGCACACGGGCGACTTGTTTGTGGCCGGGAAGATGGAAGCATCATTATTGTGCCTGCCACCCAGACGGCCATAGTTCAGCTATTGCAAGGAGAACACATGCTCAGAAGAGTTGGTAATTTGTCCTCCTGTTACTCGGTTTTTCTACGGATGCAAGGAATATTTGCATAA
Peptide sequence:
MTTCISLQEAFDKLKPCPAGIIDQLSVIPNSNEPLKVTASVYIPAHGRLVCGREDGSIIIVPATQTAIVQLLQGEHMLRRVGNLSSCYSVFLRMQGIFA
IRESfinder result
IRESfinder score
0.514324559
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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