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mmu_circWdr7_2470
Basic information of circular RNA
PanCircBase ID:
mmu_circWdr7_2470
mm39_Chr. Position ID:
chr18|63868649|63929352|+
Gene name:
Wdr7
Transcript:
ENSMUST00000072726.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
8,9,10,11,12,13,14,15,16,17,18,19
Exon/intron offsets:
0,825,3164,3526,4448,19457,24992,41949,44290,56217,58646,60579
Genomic length:
60703
Exon/intron sizes in nt:
146,103,142,249,221,196,215,770,89,96,119,124
Flanking exon/introns:
chr18:63863280-63868649|chr18:63929352-63958195
Splice length:
2470
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGTTTGATGCTGGCGACTACTCTCTGTTGTGCTCAGGTCCTAGTGAAAATGGACAGACATGGACTGGAGGGGACTTTGTGTCTGCAGACAAAGTCATCATCTGGACAGAAAATGGGCAGAGTTACATCTACAAACTCCCTGCCAGTTGCCTTCCAGCTAGTGATTCATTCCGCAGCGACGTGGGGAAAGCAGTGGAAAATTTGATCCCTCCTGTGCAGCATAGCCTCTTGGATCAGAAAGATAAAGAGTTGGTAATTTGTCCTCCTGTTACTCGGTTTTTCTACGGATGCAAGGAATATTTGCATAAGCTACTAATTCAGGGCGATTCTTCTGGAAGGTTAAATATCTGGAACATAGCAGACATAGCAGAGAAACAGGAAGCCGATGAAGGGCTAAAGATGACAACTTGTATTAGCTTGCAAGAGGCATTTGACAAGCTGAAGCCTTGCCCTGCTGGAATTATCGATCAGCTAAGTGTGATTCCCAACAGCAACGAACCTCTTAAAGTAACTGCGAGTGTCTACATACCAGCACACGGGCGACTTGTTTGTGGCCGGGAAGATGGAAGCATCATTATTGTGCCTGCCACCCAGACGGCCATAGTTCAGCTATTGCAAGGAGAACACATGCTCAGAAGAGGTTGGCCCCCTCACAGAACCCTCCGTGGCCATCGGAACAAAGTCACGTGCCTGCTGTATCCCCATCAGGTCTCAGCTCGGTATGACCAAAGATACCTGATATCTGGAGGTGTGGATTTTTCCGTCATAATTTGGGACATATTTTCTGGAGAAATGAAACATATCTTCTGTGTTCATGGTGGTGAGATCACACAACTTCTGGTCCCCCCGGAAAACTGTAGTGCAAGAGTTCAACACTGCATCTGTTCCGTAGCCAGTGACCACTCTGTAGGCCTCCTAAGTCTGCGAGAGAAAAAATGCATCATGTTGGCGTCTCGCCACCTCTTTCCTATTCAGGTGATCAAGTGGAGGCCTTCCGACGACTACCTGGTGGTGGGATGCACAGACGGCTCTGTGTATGTCTGGCAGATGGACACTGGTGCGCTGGATCGTTGTGCAATGGGAATCACAGCAGTGGAGATACTGAACGCTTGTGACGAAGCCGTTCCTGCCGCTGTAGACTCTCTTAGTCATCCAGCAGTCAACCTGAAACAAGCCATGACAAGACGGAGTCTCGCCGCCCTTAAAAATATGGCCCACCATAAGCTGCAAACCCTTGCAACGAACCTTTTGGCTTCTGAGGCCTCTGACAAGGGTAATTTACCTAAATATTCCCATAACTCCCTGATGGTTCAAGCAATAAAGACAAACCTAACAGACCCGGATATCCATGTGCTTTTCTTTGATGTGGAAGCTTTGATTATTCAACTCCTGACTGAAGAAGCCTCTAGGCCGAATACTGCACTTATTTCCCCAGAGAATCTGCAGAAAGCATCTGGCAGTTCAGACAAAGGGGGCTCTTTCCTAACTGGGAAGCGAGCAGCGGTTCTTTTCCAGCAAGTGAAAGAAACTATCAAAGAGAACATAAAGGAACACCTTCTGGATGAGGAGGAGGACGAGGAGGAGGCGAGGAGGCAGAGCAGGGAGGATAGTGACCCTGAGTACCGGGCCAGCAAGTCCAAGCCACTGACCCTACTAGAATACAACCTTACTATGGATACCGCAAAATTATTCATGTCCTGTCTCCACGCCTGGGGCTTGAATGAAGTTCTGGATGAAGTTTGTCTCGATCGCCTCGGAATGCTGAAACCGCACTGCACGGTGTCCTTTGGTCTCCTATCGAGAGGAGGTCATATGTCGCTGATGCTTCCCGGTTATAATCAGGCTGCTGGAAAACTGCTGCATGCGAAAGCAGAAGTAGGAAGGAAGCTGCCAGCAGCGGAGGGCGTAGGCAAGGGAACCTACACAGTGTCTCGAGCGGTCACCACTCAGCATCTGTTGTCCATCATATCCCTGGCGAATACTTTAATGAGTATGACCAATGCAACTTTCATTGGCGATCACATGAAGAAGGGCCCCACCAGGCCGCCTAGACCTGGCACCCCAGACCTCTCTAAGGCGAGGGATTCCCCTCCACCCTCCAGTAACATTGTGCAAGGACAGATTAAACAAGCCGCTGCGCCTGTCGTTTCTGCTCGGTCTGACGCTGATCACTCTGGCTCTGACTCTGCCTCTCCTGCTTTACCTACCTGTTTCTTAGTAAATGAAGGCTGGAGCCAGTTAGCCGCCATGCACTGTGTTATGTTGCCTGACCTGCTGGGGCTGGAGAGATTCAGGCCTCCTCTCCTGGAGATGCTAGCTCGAAGATGGCAGGACCGATGCTTGGAGGTGAGAGAGGCTGCACAGGCCCTGCTTCTAGCTGAGCTGAGAAGGATTGAGCAGGCAGGACGGAAGGAGACGATCGACACCTGGGCTCCTTACTTACCTCAGTATATGGACCATGTCATATCAC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GACGGAAGGAGACGATCGAC
59.97
CACAAAGTCCCCTCCAGTCC
59.96
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGGATTGAGCAGGCAGGAC
60.03
CACAAAGTCCCCTCCAGTCC
59.96
164
PCR template sequence:
CTTCTAGCTGAGCTGAGAAGGATTGAGCAGGCAGGACGGAAGGAGACGATCGACACCTGGGCTCCTTACTTACCTCAGTATATGGACCATGTCATATCACGTGTTTGATGCTGGCGACTACTCTCTGTTGTGCTCAGGTCCTAGTGAAAATGGACAGACATGGACTGGAGGGGACTTTGTGTCTGCAGACAAAGTCATCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
23
Interacting miRNAs (#binding sites)
mmu-miR-214-3p(2), mmu-miR-137-3p(1), mmu-miR-9-5p(1), mmu-miR-199a-5p(1), mmu-miR-103-3p(1), mmu-miR-223-3p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-709(1), mmu-miR-324-5p(1), mmu-miR-149-5p(1), mmu-miR-434-3p(1), mmu-miR-363-3p(1), mmu-miR-411-3p(1), mmu-miR-107-3p(1), mmu-miR-342-5p(1), mmu-miR-1198-5p(1), mmu-miR-410-3p(1), mmu-miR-190b-5p(1), mmu-miR-207(1), mmu-miR-5101(1), mmu-miR-338-3p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CATGTCATATCACGTGTTTGATGCTG
siRNA sets
CATCAAACACGTGATATGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWDR7_2470_ORF_1
0.9522
0.159078567
0.999999684
2481
ORF sequence:
ATGTCATATCACGTGTTTGATGCTGGCGACTACTCTCTGTTGTGCTCAGGTCCTAGTGAAAATGGACAGACATGGACTGGAGGGGACTTTGTGTCTGCAGACAAAGTCATCATCTGGACAGAAAATGGGCAGAGTTACATCTACAAACTCCCTGCCAGTTGCCTTCCAGCTAGTGATTCATTCCGCAGCGACGTGGGGAAAGCAGTGGAAAATTTGATCCCTCCTGTGCAGCATAGCCTCTTGGATCAGAAAGATAAAGAGTTGGTAATTTGTCCTCCTGTTACTCGGTTTTTCTACGGATGCAAGGAATATTTGCATAAGCTACTAATTCAGGGCGATTCTTCTGGAAGGTTAAATATCTGGAACATAGCAGACATAGCAGAGAAACAGGAAGCCGATGAAGGGCTAAAGATGACAACTTGTATTAGCTTGCAAGAGGCATTTGACAAGCTGAAGCCTTGCCCTGCTGGAATTATCGATCAGCTAAGTGTGATTCCCAACAGCAACGAACCTCTTAAAGTAACTGCGAGTGTCTACATACCAGCACACGGGCGACTTGTTTGTGGCCGGGAAGATGGAAGCATCATTATTGTGCCTGCCACCCAGACGGCCATAGTTCAGCTATTGCAAGGAGAACACATGCTCAGAAGAGGTTGGCCCCCTCACAGAACCCTCCGTGGCCATCGGAACAAAGTCACGTGCCTGCTGTATCCCCATCAGGTCTCAGCTCGGTATGACCAAAGATACCTGATATCTGGAGGTGTGGATTTTTCCGTCATAATTTGGGACATATTTTCTGGAGAAATGAAACATATCTTCTGTGTTCATGGTGGTGAGATCACACAACTTCTGGTCCCCCCGGAAAACTGTAGTGCAAGAGTTCAACACTGCATCTGTTCCGTAGCCAGTGACCACTCTGTAGGCCTCCTAAGTCTGCGAGAGAAAAAATGCATCATGTTGGCGTCTCGCCACCTCTTTCCTATTCAGGTGATCAAGTGGAGGCCTTCCGACGACTACCTGGTGGTGGGATGCACAGACGGCTCTGTGTATGTCTGGCAGATGGACACTGGTGCGCTGGATCGTTGTGCAATGGGAATCACAGCAGTGGAGATACTGAACGCTTGTGACGAAGCCGTTCCTGCCGCTGTAGACTCTCTTAGTCATCCAGCAGTCAACCTGAAACAAGCCATGACAAGACGGAGTCTCGCCGCCCTTAAAAATATGGCCCACCATAAGCTGCAAACCCTTGCAACGAACCTTTTGGCTTCTGAGGCCTCTGACAAGGGTAATTTACCTAAATATTCCCATAACTCCCTGATGGTTCAAGCAATAAAGACAAACCTAACAGACCCGGATATCCATGTGCTTTTCTTTGATGTGGAAGCTTTGATTATTCAACTCCTGACTGAAGAAGCCTCTAGGCCGAATACTGCACTTATTTCCCCAGAGAATCTGCAGAAAGCATCTGGCAGTTCAGACAAAGGGGGCTCTTTCCTAACTGGGAAGCGAGCAGCGGTTCTTTTCCAGCAAGTGAAAGAAACTATCAAAGAGAACATAAAGGAACACCTTCTGGATGAGGAGGAGGACGAGGAGGAGGCGAGGAGGCAGAGCAGGGAGGATAGTGACCCTGAGTACCGGGCCAGCAAGTCCAAGCCACTGACCCTACTAGAATACAACCTTACTATGGATACCGCAAAATTATTCATGTCCTGTCTCCACGCCTGGGGCTTGAATGAAGTTCTGGATGAAGTTTGTCTCGATCGCCTCGGAATGCTGAAACCGCACTGCACGGTGTCCTTTGGTCTCCTATCGAGAGGAGGTCATATGTCGCTGATGCTTCCCGGTTATAATCAGGCTGCTGGAAAACTGCTGCATGCGAAAGCAGAAGTAGGAAGGAAGCTGCCAGCAGCGGAGGGCGTAGGCAAGGGAACCTACACAGTGTCTCGAGCGGTCACCACTCAGCATCTGTTGTCCATCATATCCCTGGCGAATACTTTAATGAGTATGACCAATGCAACTTTCATTGGCGATCACATGAAGAAGGGCCCCACCAGGCCGCCTAGACCTGGCACCCCAGACCTCTCTAAGGCGAGGGATTCCCCTCCACCCTCCAGTAACATTGTGCAAGGACAGATTAAACAAGCCGCTGCGCCTGTCGTTTCTGCTCGGTCTGACGCTGATCACTCTGGCTCTGACTCTGCCTCTCCTGCTTTACCTACCTGTTTCTTAGTAAATGAAGGCTGGAGCCAGTTAGCCGCCATGCACTGTGTTATGTTGCCTGACCTGCTGGGGCTGGAGAGATTCAGGCCTCCTCTCCTGGAGATGCTAGCTCGAAGATGGCAGGACCGATGCTTGGAGGTGAGAGAGGCTGCACAGGCCCTGCTTCTAGCTGAGCTGAGAAGGATTGAGCAGGCAGGACGGAAGGAGACGATCGACACCTGGGCTCCTTACTTACCTCAGTATATGGACCATGTCATATCA
Peptide sequence:
MSYHVFDAGDYSLLCSGPSENGQTWTGGDFVSADKVIIWTENGQSYIYKLPASCLPASDSFRSDVGKAVENLIPPVQHSLLDQKDKELVICPPVTRFFYGCKEYLHKLLIQGDSSGRLNIWNIADIAEKQEADEGLKMTTCISLQEAFDKLKPCPAGIIDQLSVIPNSNEPLKVTASVYIPAHGRLVCGREDGSIIIVPATQTAIVQLLQGEHMLRRGWPPHRTLRGHRNKVTCLLYPHQVSARYDQRYLISGGVDFSVIIWDIFSGEMKHIFCVHGGEITQLLVPPENCSARVQHCICSVASDHSVGLLSLREKKCIMLASRHLFPIQVIKWRPSDDYLVVGCTDGSVYVWQMDTGALDRCAMGITAVEILNACDEAVPAAVDSLSHPAVNLKQAMTRRSLAALKNMAHHKLQTLATNLLASEASDKGNLPKYSHNSLMVQAIKTNLTDPDIHVLFFDVEALIIQLLTEEASRPNTALISPENLQKASGSSDKGGSFLTGKRAAVLFQQVKETIKENIKEHLLDEEEDEEEARRQSREDSDPEYRASKSKPLTLLEYNLTMDTAKLFMSCLHAWGLNEVLDEVCLDRLGMLKPHCTVSFGLLSRGGHMSLMLPGYNQAAGKLLHAKAEVGRKLPAAEGVGKGTYTVSRAVTTQHLLSIISLANTLMSMTNATFIGDHMKKGPTRPPRPGTPDLSKARDSPPPSSNIVQGQIKQAAAPVVSARSDADHSGSDSASPALPTCFLVNEGWSQLAAMHCVMLPDLLGLERFRPPLLEMLARRWQDRCLEVREAAQALLLAELRRIEQAGRKETIDTWAPYLPQYMDHVIS
IRESfinder result
IRESfinder score
0.506918781
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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