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mmu_circWdr70_326
Basic information of circular RNA
PanCircBase ID:
mmu_circWdr70_326
mm39_Chr. Position ID:
chr15|8108643|8112066|-
Gene name:
Wdr70
Transcript:
ENSMUST00000045766.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
5,4
Exon/intron offsets:
0,3302
Genomic length:
3423
Exon/intron sizes in nt:
205,121
Flanking exon/introns:
chr15:8075972-8108643|chr15:8112066-8123159
Splice length:
326
Other database IDs:
CIRCpedia: MMU_CIRCpedia_30611, circAtlas: mmu-Wdr70_0007
circAtlas: hsa-WDR70_0008
Human Ortholog IDs:
Mature spliced Sequence:
AAGCAAGAGAAAAAGAGGAAGAAATGAACAGAGAAAAAGAATTAAGAAAACAAATTGAAGATATGGAGCCAGCACCGTCAAGCTCGAGTGCAGCCAGAGAGCGCTCACAGTCATCATGCAGGGACACAAGCAGCAGTGACAGTGAGAGTGACGACAGTTCTGATTCATCTGACGATGAGCTAATCGGCCCTCCTTTACCCCCTAAAATGGTAGGAGAATCCGTAACTACTGTGGACGAAGGCACCCTTGGCCCTTTACCTCCACCTCTGTGTGAAGAAGGAGAAGATGATGATGATGACGAGTTAGACGATGAAGGAGAAGAGGAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCCTTTACCTCCACCTCTG
59.75
CGGTGCTGGCTCCATATCTT
59.89
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTTGGCCCTTTACCTCCACC
60.32
CGGTGCTGGCTCCATATCTT
59.89
158
PCR template sequence:
TACTGTGGACGAAGGCACCCTTGGCCCTTTACCTCCACCTCTGTGTGAAGAAGGAGAAGATGATGATGATGACGAGTTAGACGATGAAGGAGAAGAGGATAAGCAAGAGAAAAAGAGGAAGAAATGAACAGAGAAAAAGAATTAAGAAAACAAATTGAAGATATGGAGCCAGCACCGTCAAGCTCGAGTGCAGCCAGAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-149-5p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-377-3p(1), mmu-miR-207(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGGAGAAGAGGATAAGCAAGAGAAAA
siRNA sets
TTTTCTCTTGCTTATCCTC, CTTGCTTATCCTCTTCTCC, TTTCTCTTGCTTATCCTCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWDR70_326_ORF_1
1.0428
0.301063647
0.572774522
330
ORF sequence:
ATGAACAGAGAAAAAGAATTAAGAAAACAAATTGAAGATATGGAGCCAGCACCGTCAAGCTCGAGTGCAGCCAGAGAGCGCTCACAGTCATCATGCAGGGACACAAGCAGCAGTGACAGTGAGAGTGACGACAGTTCTGATTCATCTGACGATGAGCTAATCGGCCCTCCTTTACCCCCTAAAATGGTAGGAGAATCCGTAACTACTGTGGACGAAGGCACCCTTGGCCCTTTACCTCCACCTCTGTGTGAAGAAGGAGAAGATGATGATGATGACGAGTTAGACGATGAAGGAGAAGAGGATAAGCAAGAGAAAAAGAGGAAGAAATGA
Peptide sequence:
MNREKELRKQIEDMEPAPSSSSAARERSQSSCRDTSSSDSESDDSSDSSDDELIGPPLPPKMVGESVTTVDEGTLGPLPPPLCEEGEDDDDDELDDEGEEDKQEKKRKK
IRESfinder result
IRESfinder score
0.371245599
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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