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mmu_circWdr46_287
Basic information of circular RNA
PanCircBase ID:
mmu_circWdr46_287
mm39_Chr. Position ID:
chr17|34162531|34162910|+
Gene name:
Wdr46
Transcript:
ENSMUST00000025170.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
8,9
Exon/intron offsets:
0,243
Genomic length:
379
Exon/intron sizes in nt:
151,136
Flanking exon/introns:
chr17:34162424-34162531|chr17:34162910-34163455
Splice length:
287
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CTTCCTCCATTCTGAGGCTCTGCTCGCAGTCGCTCAGAACCGCTGGCTTTATATCTACGACAACCAAGGCATCGAGCTTCACTGCATCCGCCGCTGTGACCGAGTGACCCGGCTTGAGTTCCTCCCCTTCCACTTCCTCCTGGCCACCACTTCAGAGACAGGGTTTCTGACCTACCTGGATGTGTCAGTGGGGAAGATCGTGACAGCTCTGAACGTGCGGGCGGGACGGCTCAGTGTCATGGCTCAGAACCCCTACAATGCTGTTATCCACCTGGGACACAGTAATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGGAAGATCGTGACAGCTC
60.18
TGAAGCTCGATGCCTTGGTT
59.96
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTCAGTGTCATGGCTCAGA
59.75
TGAAGCTCGATGCCTTGGTT
59.96
140
PCR template sequence:
GTGGGGAAGATCGTGACAGCTCTGAACGTGCGGGCGGGACGGCTCAGTGTCATGGCTCAGAACCCCTACAATGCTGTTATCCACCTGGGACACAGTAATGCTTCCTCCATTCTGAGGCTCTGCTCGCAGTCGCTCAGAACCGCTGGCTTTATATCTACGACAACCAAGGCATCGAGCTTCACTGCATCCGCCGCTGTGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-103-3p(1), mmu-miR-10a-5p(1), mmu-miR-127-5p(1), mmu-miR-425-5p(1), mmu-miR-340-5p(1), mmu-miR-107-3p(1), mmu-miR-532-5p(1), mmu-miR-376a-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGACACAGTAATGCTTCCTCCATTCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWDR46_287_ORF_1
0.8839
0.116292889
0.117991661
156
ORF sequence:
ATGGCTCAGAACCCCTACAATGCTGTTATCCACCTGGGACACAGTAATGCTTCCTCCATTCTGAGGCTCTGCTCGCAGTCGCTCAGAACCGCTGGCTTTATATCTACGACAACCAAGGCATCGAGCTTCACTGCATCCGCCGCTGTGACCGAGTGA
Peptide sequence:
MAQNPYNAVIHLGHSNASSILRLCSQSLRTAGFISTTTKASSFTASAAVTE
IRESfinder result
IRESfinder score
0.521896739
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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