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mmu_circWdr33_361
Basic information of circular RNA
PanCircBase ID:
mmu_circWdr33_361
mm39_Chr. Position ID:
chr18|32011076|32013069|+
Gene name:
Wdr33
Transcript:
ENSMUST00000025264.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
8,9,10
Exon/intron offsets:
0,1647,1914
Genomic length:
1993
Exon/intron sizes in nt:
127,155,79
Flanking exon/introns:
chr18:31968534-32011076|chr18:32013069-32014224
Splice length:
361
Other database IDs:
CIRCpedia: MMU_CIRCpedia_222872, circAtlas: mmu-Wdr33_0013
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GCCATGGTGCTGATGTGAAGTGTGTAGACTGGCATCCAACCAAAGGGTTAGTCGTTTCAGGAAGTAAAGATAGCCAGCAGCCAATCAAGTTCTGGGATCCCAAGACTGGGCAGAGTCTTGCAACACTCCACGCCCACAAGAACACTGTGATGGAAGTGAAGTTAAACCTCAACGGCAATTGGCTCCTCACGGCCTCTCGGGACCATCTCTGTAAGCTCTTTGATATCCGAAACCTGAAAGAAGAGCTTCAAGTATTCCGAGGTCACAAGAAGGAGGCCACAGCTGTAGCCTGGCATCCTGTTCATGAAGGACTTTTTGCCAGTGGAGGATCTGACGGCTCTCTGTTATTCTGGCATGTTGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTAGCCTGGCATCCTGTTCA
59.75
GAACTTGATTGGCTGCTGGC
60.11
169
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTAGCCTGGCATCCTGTTC
59.75
GAACTTGATTGGCTGCTGGC
60.11
170
PCR template sequence:
GTCACAAGAAGGAGGCCACAGCTGTAGCCTGGCATCCTGTTCATGAAGGACTTTTTGCCAGTGGAGGATCTGACGGCTCTCTGTTATTCTGGCATGTTGGGCCATGGTGCTGATGTGAAGTGTGTAGACTGGCATCCAACCAAAGGGTTAGTCGTTTCAGGAAGTAAAGATAGCCAGCAGCCAATCAAGTTCTGGGATCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-122-5p(1), mmu-miR-758-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCTGGCATGTTGGGCCATGGTGCTGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWDR33_361_ORF_1
1.0483
0.207501422
0.633454131
417
ORF sequence:
ATGAAGGACTTTTTGCCAGTGGAGGATCTGACGGCTCTCTGTTATTCTGGCATGTTGGGCCATGGTGCTGATGTGAAGTGTGTAGACTGGCATCCAACCAAAGGGTTAGTCGTTTCAGGAAGTAAAGATAGCCAGCAGCCAATCAAGTTCTGGGATCCCAAGACTGGGCAGAGTCTTGCAACACTCCACGCCCACAAGAACACTGTGATGGAAGTGAAGTTAAACCTCAACGGCAATTGGCTCCTCACGGCCTCTCGGGACCATCTCTGTAAGCTCTTTGATATCCGAAACCTGAAAGAAGAGCTTCAAGTATTCCGAGGTCACAAGAAGGAGGCCACAGCTGTAGCCTGGCATCCTGTTCATGAAGGACTTTTTGCCAGTGGAGGATCTGACGGCTCTCTGTTATTCTGGCATGTT
Peptide sequence:
MKDFLPVEDLTALCYSGMLGHGADVKCVDWHPTKGLVVSGSKDSQQPIKFWDPKTGQSLATLHAHKNTVMEVKLNLNGNWLLTASRDHLCKLFDIRNLKEELQVFRGHKKEATAVAWHPVHEGLFASGGSDGSLLFWHV
IRESfinder result
IRESfinder score
0.592531842
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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