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mmu_circWdr33_2921
Basic information of circular RNA
PanCircBase ID:
mmu_circWdr33_2921
mm39_Chr. Position ID:
chr18|31968436|32035730|+
Gene name:
Wdr33
Transcript:
ENSMUST00000025264.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
7,8,9,10,11,12,13,14,15,16,17,18,19,20
Exon/intron offsets:
0,42640,44287,44554,45788,49992,50633,51271,52313,52686,57606,61217,66940,67198
Genomic length:
67294
Exon/intron sizes in nt:
98,127,155,79,111,95,87,140,153,1071,109,436,164,96
Flanking exon/introns:
chr18:31966787-31968436|chr18:32035730-32037288
Splice length:
2921
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTTCTCACCCACGGATAATAAATTTGCTACATGCTCTGATGACGGCACTGTTAGAATCTGGGATTTTCTTCGTTGCCATGAGGAAAGAATTCTCCGAGGCCATGGTGCTGATGTGAAGTGTGTAGACTGGCATCCAACCAAAGGGTTAGTCGTTTCAGGAAGTAAAGATAGCCAGCAGCCAATCAAGTTCTGGGATCCCAAGACTGGGCAGAGTCTTGCAACACTCCACGCCCACAAGAACACTGTGATGGAAGTGAAGTTAAACCTCAACGGCAATTGGCTCCTCACGGCCTCTCGGGACCATCTCTGTAAGCTCTTTGATATCCGAAACCTGAAAGAAGAGCTTCAAGTATTCCGAGGTCACAAGAAGGAGGCCACAGCTGTAGCCTGGCATCCTGTTCATGAAGGACTTTTTGCCAGTGGAGGATCTGACGGCTCTCTGTTATTCTGGCATGTTGGGGTAGAGAAGGAGGTAGGTGGGATGGAGATGGCCCACGAAGGGATGATCTGGAGTTTGGCTTGGCATCCTCTGGGACATATCCTCTGCTCAGGCTCAAATGACCATACCAGCAAATTCTGGACTCGAAACCGGCCAGGTGATAAAATGCGAGATCGATATAATCTGAACCTTTTGCCTGGCATGTCAGAAGACGGAGTGGAGTATGATGACCTGGAGCCTAATAGCCTGGCAGTGATCCCGGGGATGGGAATACCAGAACAACTGAAGTTAGCTATGGAGCAGGAACAGATGGGGAAAGATGAATCAAGTGAAATTGAAATGACAATTCCAGGCTTGGATTGGGGAATGGAGGAAGTAATGCAAAAGGATCAGAAAAAAGTCCCCCAGAAGAAAGTCCCCTATGCGAAGCCCATTCCTGCCCAGTTCCAGCAGGCCTGGATGCAAAATAAAGTCCCAATTCCTGCTCCAAATGAGGTGCTGAATGACAGGAAAGAGGATATTAAGTTAGAAGAGAAGAAAAAAACCCAAGCAGAAATTGAGCAAGAAATGGCCACATTACAGTACACCAACCCGCAGCTTTTAGAGCAACTTAAAATTGAAAGGCTTGCACAGAAACAGGCTGATCAGATCCAGCCTCCTCCCTCATCTGGGACCCCTCTCCTGGGACCTCAGCCCTTTTCTGGTCAGGGTCCGATATCTCAGATTCCTCAAGGTTTTCAGCAGCCTCATCCGTCTCAGCAGATGCCATTGGTGCCTCAAATGGGGCCTCCAGGTCCTCAGGGCCAGTTCAGAGCCCCTGGACCCCAGGGACAAATGGGACCACAAGGCCCTCCAATGCATCAAGGAGGCGGGGGCCCACAAGGATTCATGGGACCACAAGGACCCCAGGGCCCTCCCCAGGGACTGCCACGGCCTCAAGACATGCACGGTCCACAAGGAATGCAGAGGCACCCTGGACCTCATGGCCCTTTGGGTCCTCAAGGGCCACCCGGACCTCAAGGTAGTTCTGGTCCTCAAGGTCATATGGGCCCTCAGGGCCCACCAGGACCACAGGGTCACATTGGCCCCCAAGGCCCTCCTGCTTCTCAGGGTCACATGGGCCCTCAGGGTCCACCCGGTACTCAAGGAATGCAGGGACCACCTGGTCCCAGAGGAATGCAAGGACCTCCCCATCCACATGGGATACAGGGGGGCCCAGCGTCTCAAGGGATCCAGGGACCTCTGATGGGACTGAATCCAAGAGGCATGCAGGGGCCTCCAGGACCTCGGGAGAATCAGGGTCCTGCTCCTCAAGGGTTAATGATTGGCCACCCACCTCAAGAGATGAGAGGACCACATCCTCCAAGTGGATTGCTGGGACATGGCCCTCAGGAAATGAGAGGTCCTCAGGAGATGCGGGGCATGCAGGGTCCCCCACCACAGGGCTCAATGTTGGGCCCTCCCCAGGAACTTCGAGGACCTTCAGGTTCACAAGGGCAGCAGGGGCCACCCCAGGGCTCTTTGGGACCTCCACCCCAGGGTGGCATGCAAGGACCCCCTGGACCTCAGGGACAGCAGAACCCGGCAAGAGGGCCACATCCATCTCAAGGGCCAATACCGTTCCAGCAGCAGAAAGCACCTCTGCTAGGTGATGGGCCCCGGGCCCCCTTCAATCAGGAAGGACAGAGTACAGGTCCCCCACCCTTGATACCAGGCCTAGGGCAACAGGGTGCACAAGGCCGCATCCCTCCTCTGAACCCTGGACAAGGACCTGGCCCTAACAAAGGTGACACTCGAGGCCCTCCCAACCATCACCTGGGCCCCATGTCAGAGAGGCGGCATGAGCAGAGTGGTGGCCCTGAGCATGGTCCTGACAGGGGACCTTTCCGGGGTGGCCAGGACTGCAGGGGTCCTCCTGACAGGCGTGGCTCTCACCCTGATTTCCCTGATGACTTCAGACCAGATGACTTCCATCCTGACAAGCGCTTTGGCCACCGGTTACGGGAGTTTGAGGGACGAGGAGGACCCTTACCACAAGAAGAGAAGTGGAGGCGAGGGGGCCCTGGACCTCCCTTCCCACCTGATCACAGGGAATTCAATGAGGGGGATGGCCGGGGAGCAGCCCGAGGCCCCCCAGGGGCATGGGAAGGCCGCAGACCTGGAGATGACCGCTTCCCCCGGGATCCTGATGACCCGCGATTTCGAGGGCGCAGGGAAGAGAGCTTTAGAAGAGGTGCTCCCCCAAGACACGAAGGCCGCGCTCCTCCAAGAGGAAGGGACAACTTTCCTGGTCCTGATGACTTTGGTCCAGAAGAGGGTTTTGATGCTTCTGATGAAGCAGCCCGAGGACGAGATCTCCGTGGTCGAGGCCGTGGCACCCCTCGAGGAGGATCCAGGAAGTGCTTACTCCCCACTCCTGATGAGTTTCCTCGCTTTGAAGGAGGACGGAAGCCAGACTCCTGGGATGGAAATCGAGAGCCCG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGGAAGTGCTTACTCCCC
59.75
CAGTGCCGTCATCAGAGCAT
60.46
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGGAAGTGCTTACTCCCC
59.75
AACAGTGCCGTCATCAGAGC
60.67
142
PCR template sequence:
CGAGGAGGATCCAGGAAGTGCTTACTCCCCACTCCTGATGAGTTTCCTCGCTTTGAAGGAGGACGGAAGCCAGACTCCTGGGATGGAAATCGAGAGCCCGTTTCTCACCCACGGATAATAAATTTGCTACATGCTCTGATGACGGCACTGTTAGAATCTGGGATTTTCTTCGTTGCCATGAGGAAAGAATTCTCCGAGGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
21
Interacting miRNAs (#binding sites)
mmu-miR-351-5p(4), mmu-miR-296-5p(3), mmu-miR-298-5p(3), mmu-miR-328-3p(2), mmu-miR-125b-5p(2), mmu-miR-125a-5p(2), mmu-miR-370-3p(2), mmu-miR-342-5p(2), mmu-miR-1904(2), mmu-miR-9-5p(1), mmu-miR-122-5p(1), mmu-miR-33-5p(1), mmu-miR-320-3p(1), mmu-miR-339-5p(1), mmu-miR-10a-5p(1), mmu-miR-758-3p(1), mmu-miR-383-5p(1), mmu-miR-680(1), mmu-miR-3470b(1), mmu-miR-670-5p(1), mmu-miR-490-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AATCGAGAGCCCGTTTCTCACCCACG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWDR33_2921_ORF_1
1.0751
0.198811236
0.999999976
2790
ORF sequence:
ATGGAAGTGAAGTTAAACCTCAACGGCAATTGGCTCCTCACGGCCTCTCGGGACCATCTCTGTAAGCTCTTTGATATCCGAAACCTGAAAGAAGAGCTTCAAGTATTCCGAGGTCACAAGAAGGAGGCCACAGCTGTAGCCTGGCATCCTGTTCATGAAGGACTTTTTGCCAGTGGAGGATCTGACGGCTCTCTGTTATTCTGGCATGTTGGGGTAGAGAAGGAGGTAGGTGGGATGGAGATGGCCCACGAAGGGATGATCTGGAGTTTGGCTTGGCATCCTCTGGGACATATCCTCTGCTCAGGCTCAAATGACCATACCAGCAAATTCTGGACTCGAAACCGGCCAGGTGATAAAATGCGAGATCGATATAATCTGAACCTTTTGCCTGGCATGTCAGAAGACGGAGTGGAGTATGATGACCTGGAGCCTAATAGCCTGGCAGTGATCCCGGGGATGGGAATACCAGAACAACTGAAGTTAGCTATGGAGCAGGAACAGATGGGGAAAGATGAATCAAGTGAAATTGAAATGACAATTCCAGGCTTGGATTGGGGAATGGAGGAAGTAATGCAAAAGGATCAGAAAAAAGTCCCCCAGAAGAAAGTCCCCTATGCGAAGCCCATTCCTGCCCAGTTCCAGCAGGCCTGGATGCAAAATAAAGTCCCAATTCCTGCTCCAAATGAGGTGCTGAATGACAGGAAAGAGGATATTAAGTTAGAAGAGAAGAAAAAAACCCAAGCAGAAATTGAGCAAGAAATGGCCACATTACAGTACACCAACCCGCAGCTTTTAGAGCAACTTAAAATTGAAAGGCTTGCACAGAAACAGGCTGATCAGATCCAGCCTCCTCCCTCATCTGGGACCCCTCTCCTGGGACCTCAGCCCTTTTCTGGTCAGGGTCCGATATCTCAGATTCCTCAAGGTTTTCAGCAGCCTCATCCGTCTCAGCAGATGCCATTGGTGCCTCAAATGGGGCCTCCAGGTCCTCAGGGCCAGTTCAGAGCCCCTGGACCCCAGGGACAAATGGGACCACAAGGCCCTCCAATGCATCAAGGAGGCGGGGGCCCACAAGGATTCATGGGACCACAAGGACCCCAGGGCCCTCCCCAGGGACTGCCACGGCCTCAAGACATGCACGGTCCACAAGGAATGCAGAGGCACCCTGGACCTCATGGCCCTTTGGGTCCTCAAGGGCCACCCGGACCTCAAGGTAGTTCTGGTCCTCAAGGTCATATGGGCCCTCAGGGCCCACCAGGACCACAGGGTCACATTGGCCCCCAAGGCCCTCCTGCTTCTCAGGGTCACATGGGCCCTCAGGGTCCACCCGGTACTCAAGGAATGCAGGGACCACCTGGTCCCAGAGGAATGCAAGGACCTCCCCATCCACATGGGATACAGGGGGGCCCAGCGTCTCAAGGGATCCAGGGACCTCTGATGGGACTGAATCCAAGAGGCATGCAGGGGCCTCCAGGACCTCGGGAGAATCAGGGTCCTGCTCCTCAAGGGTTAATGATTGGCCACCCACCTCAAGAGATGAGAGGACCACATCCTCCAAGTGGATTGCTGGGACATGGCCCTCAGGAAATGAGAGGTCCTCAGGAGATGCGGGGCATGCAGGGTCCCCCACCACAGGGCTCAATGTTGGGCCCTCCCCAGGAACTTCGAGGACCTTCAGGTTCACAAGGGCAGCAGGGGCCACCCCAGGGCTCTTTGGGACCTCCACCCCAGGGTGGCATGCAAGGACCCCCTGGACCTCAGGGACAGCAGAACCCGGCAAGAGGGCCACATCCATCTCAAGGGCCAATACCGTTCCAGCAGCAGAAAGCACCTCTGCTAGGTGATGGGCCCCGGGCCCCCTTCAATCAGGAAGGACAGAGTACAGGTCCCCCACCCTTGATACCAGGCCTAGGGCAACAGGGTGCACAAGGCCGCATCCCTCCTCTGAACCCTGGACAAGGACCTGGCCCTAACAAAGGTGACACTCGAGGCCCTCCCAACCATCACCTGGGCCCCATGTCAGAGAGGCGGCATGAGCAGAGTGGTGGCCCTGAGCATGGTCCTGACAGGGGACCTTTCCGGGGTGGCCAGGACTGCAGGGGTCCTCCTGACAGGCGTGGCTCTCACCCTGATTTCCCTGATGACTTCAGACCAGATGACTTCCATCCTGACAAGCGCTTTGGCCACCGGTTACGGGAGTTTGAGGGACGAGGAGGACCCTTACCACAAGAAGAGAAGTGGAGGCGAGGGGGCCCTGGACCTCCCTTCCCACCTGATCACAGGGAATTCAATGAGGGGGATGGCCGGGGAGCAGCCCGAGGCCCCCCAGGGGCATGGGAAGGCCGCAGACCTGGAGATGACCGCTTCCCCCGGGATCCTGATGACCCGCGATTTCGAGGGCGCAGGGAAGAGAGCTTTAGAAGAGGTGCTCCCCCAAGACACGAAGGCCGCGCTCCTCCAAGAGGAAGGGACAACTTTCCTGGTCCTGATGACTTTGGTCCAGAAGAGGGTTTTGATGCTTCTGATGAAGCAGCCCGAGGACGAGATCTCCGTGGTCGAGGCCGTGGCACCCCTCGAGGAGGATCCAGGAAGTGCTTACTCCCCACTCCTGATGAGTTTCCTCGCTTTGAAGGAGGACGGAAGCCAGACTCCTGGGATGGAAATCGAGAGCCCGTTTCTCACCCACGGATAATAAATTTGCTACATGCTCTGATGACGGCACTGTTAGAATCTGGGATTTTCTTCGTTGCCATGAGGAAAGAATTCTCCGAGGCCATGGTGCTGATGTGA
Peptide sequence:
MEVKLNLNGNWLLTASRDHLCKLFDIRNLKEELQVFRGHKKEATAVAWHPVHEGLFASGGSDGSLLFWHVGVEKEVGGMEMAHEGMIWSLAWHPLGHILCSGSNDHTSKFWTRNRPGDKMRDRYNLNLLPGMSEDGVEYDDLEPNSLAVIPGMGIPEQLKLAMEQEQMGKDESSEIEMTIPGLDWGMEEVMQKDQKKVPQKKVPYAKPIPAQFQQAWMQNKVPIPAPNEVLNDRKEDIKLEEKKKTQAEIEQEMATLQYTNPQLLEQLKIERLAQKQADQIQPPPSSGTPLLGPQPFSGQGPISQIPQGFQQPHPSQQMPLVPQMGPPGPQGQFRAPGPQGQMGPQGPPMHQGGGGPQGFMGPQGPQGPPQGLPRPQDMHGPQGMQRHPGPHGPLGPQGPPGPQGSSGPQGHMGPQGPPGPQGHIGPQGPPASQGHMGPQGPPGTQGMQGPPGPRGMQGPPHPHGIQGGPASQGIQGPLMGLNPRGMQGPPGPRENQGPAPQGLMIGHPPQEMRGPHPPSGLLGHGPQEMRGPQEMRGMQGPPPQGSMLGPPQELRGPSGSQGQQGPPQGSLGPPPQGGMQGPPGPQGQQNPARGPHPSQGPIPFQQQKAPLLGDGPRAPFNQEGQSTGPPPLIPGLGQQGAQGRIPPLNPGQGPGPNKGDTRGPPNHHLGPMSERRHEQSGGPEHGPDRGPFRGGQDCRGPPDRRGSHPDFPDDFRPDDFHPDKRFGHRLREFEGRGGPLPQEEKWRRGGPGPPFPPDHREFNEGDGRGAARGPPGAWEGRRPGDDRFPRDPDDPRFRGRREESFRRGAPPRHEGRAPPRGRDNFPGPDDFGPEEGFDASDEAARGRDLRGRGRGTPRGGSRKCLLPTPDEFPRFEGGRKPDSWDGNREPVSHPRIINLLHALMTALLESGIFFVAMRKEFSEAMVLM
IRESfinder result
IRESfinder score
0.379193734
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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