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mmu_circWdfy3_985
Basic information of circular RNA
PanCircBase ID:
mmu_circWdfy3_985
mm39_Chr. Position ID:
chr5|102044264|102054064|-
Gene name:
Wdfy3
Transcript:
ENSMUST00000053177.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
35,34,33,32,31,30
Exon/intron offsets:
0,2028,3615,5372,6041,9705
Genomic length:
9800
Exon/intron sizes in nt:
180,135,236,218,121,95
Flanking exon/introns:
chr5:102043010-102044264|chr5:102054064-102055314
Splice length:
985
Other database IDs:
circAtlas: mmu-Wdfy3_0032
Human Ortholog IDs:
Mature spliced Sequence:
GTTTGGCCAGTTCATTTCTTCTACTTTGCCCACCTTTGCCGTTTGTGAGAAATTCGTGGTTATGGAAATAAATAATGAAGAGAAACCAGACCCGGGAGCAGAAGAGGAGTTTGGAGGTCTTGTGTCTGCTAACCTTATACTTTTGAGGAACAGGCTCCTAGACATCTTGCTAAAGCTGGTTTATACATCTAAAGAAAAGACAAACATTAACTTACAGGCTTGTGAAGAGCTGGTCAGGACACTGGGTTTCGACTGGATCATGATGTTCATGGAAGAGCACTTGCATCCCACCACAGTCACGGCTGCCATGAGGATTCTCGTGGTCCTGCTGAGTAACCAGTCCATTCTAATCAAGTTTAAAGAGGGACTCAGTGGCGGGGGCTGGCTGGAGCAGACAGATTCCGTCCTGACAAACAAGATTGGAACTGTGTTAGGATTCAATGTGGGCAGGAGTGCCGGAGGGAGATCCACGGTCAGGGAGATTAACCGGGACGCCTGCCATTTTCCCGGCTTTCTGGTTCTCCAGTCTTTCCTCCCTAAGCACACGAATGTCCCTGCCCTCTATTTTCTCCTTATGGCCTTGTTTCTGCAACAGCCAGTGAGCGAGCTGCCCGAGAACCTGCAGGTCAGTGTGCCTGTCACCAGCAGCCGATGTAAGCAGGGTTGCCAGTTTGATCTGGATTCCATCTGGACATTTATCTTTGGCGTCCCTGCCTCTAGCGGAACGGTGGTCTCTTCCATCCACAACGTGTGCACAGAGTCTGCATTCCTGCTGCTTGGGATGCTGCGCAGCATGCTGAACTCGCCATGGCAGTCGGAAGAGGAGGGCTCGTGGCTCCGAGAGTATCCAGTGACCCTGATGCAGTTCTTTAGGTACCTGTACCACAACGTCCCAGACCTCGCCTCCATGTGGCTGAGCCCCGACTTCCTGTGTGCCTTAGCAGCCACCGTCTTCCCCTTCAACATCCGCCCGTACTCAGAGATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCGACTTCCTGTGTGCCTTA
59.68
CCCGGGTCTGGTTTCTCTTC
60.04
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTTAGCAGCCACCGTCTTCC
60.74
CCCGGGTCTGGTTTCTCTTC
60.04
145
PCR template sequence:
CAACGTCCCAGACCTCGCCTCCATGTGGCTGAGCCCCGACTTCCTGTGTGCCTTAGCAGCCACCGTCTTCCCCTTCAACATCCGCCCGTACTCAGAGATGGTTTGGCCAGTTCATTTCTTCTACTTTGCCCACCTTTGCCGTTTGTGAGAAATTCGTGGTTATGGAAATAAATAATGAAGAGAAACCAGACCCGGGAGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
13
Interacting miRNAs (#binding sites)
mmu-miR-124-3p(1), mmu-miR-199a-5p(1), mmu-miR-322-5p(1), mmu-miR-217-5p(1), mmu-miR-214-3p(1), mmu-miR-24-3p(1), mmu-miR-503-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-27a-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-1199-5p(1), mmu-miR-670-5p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
GTACTCAGAGATGGTTTGGCCAGTTC
siRNA sets
GAACTGGCCAAACCATCTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWDFY3_985_ORF_1
1.2312
0.217621159
0.992641315
972
ORF sequence:
ATGGAAATAAATAATGAAGAGAAACCAGACCCGGGAGCAGAAGAGGAGTTTGGAGGTCTTGTGTCTGCTAACCTTATACTTTTGAGGAACAGGCTCCTAGACATCTTGCTAAAGCTGGTTTATACATCTAAAGAAAAGACAAACATTAACTTACAGGCTTGTGAAGAGCTGGTCAGGACACTGGGTTTCGACTGGATCATGATGTTCATGGAAGAGCACTTGCATCCCACCACAGTCACGGCTGCCATGAGGATTCTCGTGGTCCTGCTGAGTAACCAGTCCATTCTAATCAAGTTTAAAGAGGGACTCAGTGGCGGGGGCTGGCTGGAGCAGACAGATTCCGTCCTGACAAACAAGATTGGAACTGTGTTAGGATTCAATGTGGGCAGGAGTGCCGGAGGGAGATCCACGGTCAGGGAGATTAACCGGGACGCCTGCCATTTTCCCGGCTTTCTGGTTCTCCAGTCTTTCCTCCCTAAGCACACGAATGTCCCTGCCCTCTATTTTCTCCTTATGGCCTTGTTTCTGCAACAGCCAGTGAGCGAGCTGCCCGAGAACCTGCAGGTCAGTGTGCCTGTCACCAGCAGCCGATGTAAGCAGGGTTGCCAGTTTGATCTGGATTCCATCTGGACATTTATCTTTGGCGTCCCTGCCTCTAGCGGAACGGTGGTCTCTTCCATCCACAACGTGTGCACAGAGTCTGCATTCCTGCTGCTTGGGATGCTGCGCAGCATGCTGAACTCGCCATGGCAGTCGGAAGAGGAGGGCTCGTGGCTCCGAGAGTATCCAGTGACCCTGATGCAGTTCTTTAGGTACCTGTACCACAACGTCCCAGACCTCGCCTCCATGTGGCTGAGCCCCGACTTCCTGTGTGCCTTAGCAGCCACCGTCTTCCCCTTCAACATCCGCCCGTACTCAGAGATGGTTTGGCCAGTTCATTTCTTCTACTTTGCCCACCTTTGCCGTTTGTGA
Peptide sequence:
MEINNEEKPDPGAEEEFGGLVSANLILLRNRLLDILLKLVYTSKEKTNINLQACEELVRTLGFDWIMMFMEEHLHPTTVTAAMRILVVLLSNQSILIKFKEGLSGGGWLEQTDSVLTNKIGTVLGFNVGRSAGGRSTVREINRDACHFPGFLVLQSFLPKHTNVPALYFLLMALFLQQPVSELPENLQVSVPVTSSRCKQGCQFDLDSIWTFIFGVPASSGTVVSSIHNVCTESAFLLLGMLRSMLNSPWQSEEEGSWLREYPVTLMQFFRYLYHNVPDLASMWLSPDFLCALAATVFPFNIRPYSEMVWPVHFFYFAHLCRL
IRESfinder result
IRESfinder score
0.665866191
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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