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mmu_circWashc5_1101
Basic information of circular RNA
PanCircBase ID:
mmu_circWashc5_1101
mm39_Chr. Position ID:
chr15|59217186|59224479|-
Gene name:
Washc5
Transcript:
ENSMUST00000022976.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
19,18,17,16,15,14,13,12,11
Exon/intron offsets:
0,876,1482,3047,3955,4794,4949,6134,7163
Genomic length:
7293
Exon/intron sizes in nt:
18,01,02,81,14,11,11,70,00,00,00,000
Flanking exon/introns:
chr15:59215998-59217186|chr15:59224479-59227682
Splice length:
1101
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATGTTCAAGCAAATGCTTTCCGAGAAACAGTCCAAGTGGGAGCATTACAAGAAGGAGGGCTCTGAGCGCATGACCGAGCTTGCTGACGTCTTTTCAGGGGTGAAACCACTAACCCGAGTGGAGAAAAACGAAAATCTTCAAGCTTGGTTCAGAGAGATCTCAAAACAAATACTGTCTCTAAATTATGATGATTCTACTGCTGCAGGCAGAAAAACTGTGCAACTAATACAAGCTCTGGAAGAGGTTCAGGAGTTTCACCAGTTGGAATCAAATCTACAAGTTTGCCAGTTTCTAGCTGACACCCGAAAGTTTCTCCATCAAATGATCAGAACCATCAACATCAAGGAGGAAGTCCTGATCACAGTGCAGATCATCGGGGACCTTTCCTTTGCCTGGCAGTTAATTGACAGTTTCACATCCATCATGCAAGAAAGCATAAGGGTAAACCCATCCATGGTTACCAAACTCAGAGCCACGTTCCTAAAGCTTGCCTCTGCCCTTGACCTTCCCCTGCTACGTATTAATCAAGCAAATAGCCCTGATCTCCTCAGTGTGTCTCAGTATTATTCAGGAGAATTGGTGTCATACGTGAGAAAGGTTTTGCAGATCATTCCAGAAAGCATGTTCACATCTCTCCTGAAGATCATAAAGCTTCAGACCCATGACATCATGGAAGTGCCTACACGCCTGGACAAGGACAAGCTGCGGGACTACGCTCAGCTTGGCCCGAGATATGAGGTTGCCAAGCTCACTCACGCCATCTCCATCTTTACTGAGGGCATCTTGATGATGAAAACGACCCTGGTGGGCATCATCAAGGTGGATCCCAAGCAGTTGCTGGAAGACGGAATAAGGAAAGAGCTGGTTAAACGCGTGGCTTTCGCCCTTCACCGGGGACTGATATTCAACCCTCGTGCCAAGCCAAGTGAGTTGATGCCCAAACTGAAAGAGCTGGGGGCCACCATGGATGGGTTCCATCGCTCCTTTGAGTATATACAGGACTACGTCAGCATTTACGGTCTGAAGATTTGGCAGGAGGAAGTCTCTCGTATTATAAATTATAACGTGGAACAGGAGTGTAATAACTTCTTAAGAACAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGGTCTGAAGATTTGGCAGG
58.91
GCTCAGAGCCCTCCTTCTTG
60.11
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCAGGAGGAAGTCTCTCG
60.97
CCCCTGAAAAGACGTCAGCA
60.25
172
PCR template sequence:
CTACGTCAGCATTTACGGTCTGAAGATTTGGCAGGAGGAAGTCTCTCGTATTATAAATTATAACGTGGAACAGGAGTGTAATAACTTCTTAAGAACAAAGATGTTCAAGCAAATGCTTTCCGAGAAACAGTCCAAGTGGGAGCATTACAAGAAGGAGGGCTCTGAGCGCATGACCGAGCTTGCTGACGTCTTTTCAGGGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-140-5p(1), mmu-miR-200c-3p(1), mmu-miR-18a-5p(1), mmu-miR-298-5p(1), mmu-miR-22-3p(1), mmu-miR-182-5p(1), mmu-miR-425-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-335-3p(1), mmu-miR-3087-3p(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTTAAGAACAAAGATGTTCAAGCAAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWASHC5_1101_ORF_1
1.261
0.257558052
0.999999163
2202
ORF sequence:
ATGTTCAAGCAAATGCTTTCCGAGAAACAGTCCAAGTGGGAGCATTACAAGAAGGAGGGCTCTGAGCGCATGACCGAGCTTGCTGACGTCTTTTCAGGGGTGAAACCACTAACCCGAGTGGAGAAAAACGAAAATCTTCAAGCTTGGTTCAGAGAGATCTCAAAACAAATACTGTCTCTAAATTATGATGATTCTACTGCTGCAGGCAGAAAAACTGTGCAACTAATACAAGCTCTGGAAGAGGTTCAGGAGTTTCACCAGTTGGAATCAAATCTACAAGTTTGCCAGTTTCTAGCTGACACCCGAAAGTTTCTCCATCAAATGATCAGAACCATCAACATCAAGGAGGAAGTCCTGATCACAGTGCAGATCATCGGGGACCTTTCCTTTGCCTGGCAGTTAATTGACAGTTTCACATCCATCATGCAAGAAAGCATAAGGGTAAACCCATCCATGGTTACCAAACTCAGAGCCACGTTCCTAAAGCTTGCCTCTGCCCTTGACCTTCCCCTGCTACGTATTAATCAAGCAAATAGCCCTGATCTCCTCAGTGTGTCTCAGTATTATTCAGGAGAATTGGTGTCATACGTGAGAAAGGTTTTGCAGATCATTCCAGAAAGCATGTTCACATCTCTCCTGAAGATCATAAAGCTTCAGACCCATGACATCATGGAAGTGCCTACACGCCTGGACAAGGACAAGCTGCGGGACTACGCTCAGCTTGGCCCGAGATATGAGGTTGCCAAGCTCACTCACGCCATCTCCATCTTTACTGAGGGCATCTTGATGATGAAAACGACCCTGGTGGGCATCATCAAGGTGGATCCCAAGCAGTTGCTGGAAGACGGAATAAGGAAAGAGCTGGTTAAACGCGTGGCTTTCGCCCTTCACCGGGGACTGATATTCAACCCTCGTGCCAAGCCAAGTGAGTTGATGCCCAAACTGAAAGAGCTGGGGGCCACCATGGATGGGTTCCATCGCTCCTTTGAGTATATACAGGACTACGTCAGCATTTACGGTCTGAAGATTTGGCAGGAGGAAGTCTCTCGTATTATAAATTATAACGTGGAACAGGAGTGTAATAACTTCTTAAGAACAAAGATGTTCAAGCAAATGCTTTCCGAGAAACAGTCCAAGTGGGAGCATTACAAGAAGGAGGGCTCTGAGCGCATGACCGAGCTTGCTGACGTCTTTTCAGGGGTGAAACCACTAACCCGAGTGGAGAAAAACGAAAATCTTCAAGCTTGGTTCAGAGAGATCTCAAAACAAATACTGTCTCTAAATTATGATGATTCTACTGCTGCAGGCAGAAAAACTGTGCAACTAATACAAGCTCTGGAAGAGGTTCAGGAGTTTCACCAGTTGGAATCAAATCTACAAGTTTGCCAGTTTCTAGCTGACACCCGAAAGTTTCTCCATCAAATGATCAGAACCATCAACATCAAGGAGGAAGTCCTGATCACAGTGCAGATCATCGGGGACCTTTCCTTTGCCTGGCAGTTAATTGACAGTTTCACATCCATCATGCAAGAAAGCATAAGGGTAAACCCATCCATGGTTACCAAACTCAGAGCCACGTTCCTAAAGCTTGCCTCTGCCCTTGACCTTCCCCTGCTACGTATTAATCAAGCAAATAGCCCTGATCTCCTCAGTGTGTCTCAGTATTATTCAGGAGAATTGGTGTCATACGTGAGAAAGGTTTTGCAGATCATTCCAGAAAGCATGTTCACATCTCTCCTGAAGATCATAAAGCTTCAGACCCATGACATCATGGAAGTGCCTACACGCCTGGACAAGGACAAGCTGCGGGACTACGCTCAGCTTGGCCCGAGATATGAGGTTGCCAAGCTCACTCACGCCATCTCCATCTTTACTGAGGGCATCTTGATGATGAAAACGACCCTGGTGGGCATCATCAAGGTGGATCCCAAGCAGTTGCTGGAAGACGGAATAAGGAAAGAGCTGGTTAAACGCGTGGCTTTCGCCCTTCACCGGGGACTGATATTCAACCCTCGTGCCAAGCCAAGTGAGTTGATGCCCAAACTGAAAGAGCTGGGGGCCACCATGGATGGGTTCCATCGCTCCTTTGAGTATATACAGGACTACGTCAGCATTTACGGTCTGAAGATTTGGCAGGAGGAAGTCTCTCGTATTATAAATTATAACGTGGAACAGGAGTGTAATAACTTCTTAAGAACAAAG
Peptide sequence:
MFKQMLSEKQSKWEHYKKEGSERMTELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEVQEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITVQIIGDLSFAWQLIDSFTSIMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQIIPESMFTSLLKIIKLQTHDIMEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPKLKELGATMDGFHRSFEYIQDYVSIYGLKIWQEEVSRIINYNVEQECNNFLRTKMFKQMLSEKQSKWEHYKKEGSERMTELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEVQEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITVQIIGDLSFAWQLIDSFTSIMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQIIPESMFTSLLKIIKLQTHDIMEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPKLKELGATMDGFHRSFEYIQDYVSIYGLKIWQEEVSRIINYNVEQECNNFLRTK
IRESfinder result
IRESfinder score
0.622479011
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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