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mmu_circWac_582
Basic information of circular RNA
PanCircBase ID:
mmu_circWac_582
mm39_Chr. Position ID:
chr18|7901495|7916297|+
Gene name:
Wac
Transcript:
ENSMUST00000171042.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
4,5,6,7
Exon/intron offsets:
0,3904,4436,14556
Genomic length:
14802
Exon/intron sizes in nt:
1,07,11,61,13,246
Flanking exon/introns:
chr18:7871744-7901495|chr18:7916297-7917564
Splice length:
582
Other database IDs:
CIRCpedia: MMU_CIRCpedia_228037, circAtlas: mmu-Wac_0012
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GGACCAGTTACTCTCCACAAGAAAACTCACACAACCACAGTGCACTTCATAGTTCAAATTCACATTCTTCTAATCCAAGCAATAATCCAAGCAAAACTTCAGATGCACCTTATGATTCTGCAGATGATTGGTCTGAGCACATTAGCTCATCTGGAAAAAAGTACTACTACAATTGTCGCACAGAAGTTTCACAGTGGGAGAAACCAAAAGAGTGGCTTGAAAGAGAACAGAGACAAAAAGAAGCAAATAAGCTGGCAGTTAATAGTTTCCCAAAAGACAGGGATTACAGAAGAGAGGTGATGCAAGCAACAGCCACTAGTGGGTTCACCAGTGGAAAATCTGCTCCAGGAGACAAATCAATATCACATTCTTGCACAACTCCTTCCACATCTTCAGCCTCTGGCCTGAACCCTACATCTGCGCCTCCGACATCTGCTTCAGCAGTACCTGTTTCTCCCGTTCCACAATCAACAATACCTCCATTACTTCAGGACCCAAATCTTTTTAGACAGTTGCTTCCTGCTTTGCAAGCCACGCTACAGCTTAATAATTCTAATGTGGACATATCCAAAATAAATGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTTTGCAAGCCACGCTACAG
60.11
TGCTTGGATTATTGCTTGGA
55.3
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTTTGCAAGCCACGCTACAG
60.11
TTGCTTGGATTATTGCTTGGA
56.11
154
PCR template sequence:
TTACTTCAGGACCCAAATCTTTTTAGACAGTTGCTTCCTGCTTTGCAAGCCACGCTACAGCTTAATAATTCTAATGTGGACATATCCAAAATAAATGAAGGGACCAGTTACTCTCCACAAGAAAACTCACACAACCACAGTGCACTTCATAGTTCAAATTCACATTCTTCTAATCCAAGCAATAATCCAAGCAAAACTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-137-3p(1), mmu-miR-18a-5p(1), mmu-miR-339-5p(1), mmu-miR-676-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-190b-5p(1), mmu-miR-202-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAAATAAATGAAGGGACCAGTTACTC
siRNA sets
TAACTGGTCCCTTCATTTA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWAC_582_ORF_1
1.0681
-0.054803562
0.938590966
864
ORF sequence:
ATGCAAGCAACAGCCACTAGTGGGTTCACCAGTGGAAAATCTGCTCCAGGAGACAAATCAATATCACATTCTTGCACAACTCCTTCCACATCTTCAGCCTCTGGCCTGAACCCTACATCTGCGCCTCCGACATCTGCTTCAGCAGTACCTGTTTCTCCCGTTCCACAATCAACAATACCTCCATTACTTCAGGACCCAAATCTTTTTAGACAGTTGCTTCCTGCTTTGCAAGCCACGCTACAGCTTAATAATTCTAATGTGGACATATCCAAAATAAATGAAGGGACCAGTTACTCTCCACAAGAAAACTCACACAACCACAGTGCACTTCATAGTTCAAATTCACATTCTTCTAATCCAAGCAATAATCCAAGCAAAACTTCAGATGCACCTTATGATTCTGCAGATGATTGGTCTGAGCACATTAGCTCATCTGGAAAAAAGTACTACTACAATTGTCGCACAGAAGTTTCACAGTGGGAGAAACCAAAAGAGTGGCTTGAAAGAGAACAGAGACAAAAAGAAGCAAATAAGCTGGCAGTTAATAGTTTCCCAAAAGACAGGGATTACAGAAGAGAGGTGATGCAAGCAACAGCCACTAGTGGGTTCACCAGTGGAAAATCTGCTCCAGGAGACAAATCAATATCACATTCTTGCACAACTCCTTCCACATCTTCAGCCTCTGGCCTGAACCCTACATCTGCGCCTCCGACATCTGCTTCAGCAGTACCTGTTTCTCCCGTTCCACAATCAACAATACCTCCATTACTTCAGGACCCAAATCTTTTTAGACAGTTGCTTCCTGCTTTGCAAGCCACGCTACAGCTTAATAATTCTAATGTGGACATATCCAAAATAAATGAA
Peptide sequence:
MQATATSGFTSGKSAPGDKSISHSCTTPSTSSASGLNPTSAPPTSASAVPVSPVPQSTIPPLLQDPNLFRQLLPALQATLQLNNSNVDISKINEGTSYSPQENSHNHSALHSSNSHSSNPSNNPSKTSDAPYDSADDWSEHISSSGKKYYYNCRTEVSQWEKPKEWLEREQRQKEANKLAVNSFPKDRDYRREVMQATATSGFTSGKSAPGDKSISHSCTTPSTSSASGLNPTSAPPTSASAVPVSPVPQSTIPPLLQDPNLFRQLLPALQATLQLNNSNVDISKINE
IRESfinder result
IRESfinder score
0.815423686
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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