top of page
mmu_circWac_578
Basic information of circular RNA
PanCircBase ID:
mmu_circWac_578
mm39_Chr. Position ID:
chr18|7917552|7921608|+
Gene name:
Wac
Transcript:
ENSMUST00000074919.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
9,10,11,12
Exon/intron offsets:
0,880,2579,3869
Genomic length:
4056
Exon/intron sizes in nt:
1,23,14,91,19,187
Flanking exon/introns:
chr18:7916297-7917552|chr18:7921608-7921855
Splice length:
578
Other database IDs:
CIRCpedia: MMU_CIRCpedia_30083, circAtlas: mmu-Wac_0037
Human Ortholog IDs:
Mature spliced Sequence:
TTCTTACAGCAGCTGTAACACAAGCTTCCCTGCAGTCTATCATCCACAAGTTTCTTACTGCTGGACCATCTGCTTTCAACATCACCTCACTGATTTCTCAAGCTGCCCAGCTTTCTACACAAGCCCAGCCATCTAATCAGTCTCCAATGTCTCTGACATCTGATGCTTCATCCCCAAGATCCTATGTGTCTCCAAGAATAAGCACACCTCAAACTAATACAGTCCCTATGAAACCTTTGATTAGTACTCCACCTGTTTCATCCCAGCCAAAGGTTAGTACTCCAGTAGTTAAGCAAGGACCAGTGTCACATTCGGCTACACAGCAGCCTGTAACTGCTGACAAGCAGCAGAGCCATGATCCTGTTTCTCCTCGAAGTCTTCAGCGCTTAAGTAGCCAGAGAAGTCCATCACCTGGTCCAAATCATACTTGTAGTAGTAATGCATCAACTGCAACTGTTGTACCACAGAATGCATCTGCTAGACCTGCGTGCTCATTAACACCTACTCTAGCAGCGCACTTCAATGATAATCTCATAAAGCATGTTCAAGGGTGGCCTGCAGACCACGCAGAGAAACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AACACCTACTCTAGCAGCGC
59.83
TGAAAGCAGATGGTCCAGCA
59.6
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACACCTACTCTAGCAGCGC
59.83
AAAGCAGATGGTCCAGCAGT
59.6
158
PCR template sequence:
TAGACCTGCGTGCTCATTAACACCTACTCTAGCAGCGCACTTCAATGATAATCTCATAAAGCATGTTCAAGGGTGGCCTGCAGACCACGCAGAGAAACAGTTCTTACAGCAGCTGTAACACAAGCTTCCCTGCAGTCTATCATCCACAAGTTTCTTACTGCTGGACCATCTGCTTTCAACATCACCTCACTGATTTCTCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-320-3p(1), mmu-miR-218-5p(1), mmu-miR-539-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CGCAGAGAAACAGTTCTTACAGCAGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCWAC_578_ORF_1
0.9286
-0.071767791
0.35678575
450
ORF sequence:
ATGTCTCTGACATCTGATGCTTCATCCCCAAGATCCTATGTGTCTCCAAGAATAAGCACACCTCAAACTAATACAGTCCCTATGAAACCTTTGATTAGTACTCCACCTGTTTCATCCCAGCCAAAGGTTAGTACTCCAGTAGTTAAGCAAGGACCAGTGTCACATTCGGCTACACAGCAGCCTGTAACTGCTGACAAGCAGCAGAGCCATGATCCTGTTTCTCCTCGAAGTCTTCAGCGCTTAAGTAGCCAGAGAAGTCCATCACCTGGTCCAAATCATACTTGTAGTAGTAATGCATCAACTGCAACTGTTGTACCACAGAATGCATCTGCTAGACCTGCGTGCTCATTAACACCTACTCTAGCAGCGCACTTCAATGATAATCTCATAAAGCATGTTCAAGGGTGGCCTGCAGACCACGCAGAGAAACAGTTCTTACAGCAGCTGTAA
Peptide sequence:
MSLTSDASSPRSYVSPRISTPQTNTVPMKPLISTPPVSSQPKVSTPVVKQGPVSHSATQQPVTADKQQSHDPVSPRSLQRLSSQRSPSPGPNHTCSSNASTATVVPQNASARPACSLTPTLAAHFNDNLIKHVQGWPADHAEKQFLQQL
IRESfinder result
IRESfinder score
0.726191192
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page