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mmu_circVwa3b_346
Basic information of circular RNA
PanCircBase ID:
mmu_circVwa3b_346
mm39_Chr. Position ID:
chr1|37081375|37084289|+
Gene name:
Vwa3b
Transcript:
ENSMUST00000117172.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,4
Exon/intron offsets:
0,2663
Genomic length:
2914
Exon/intron sizes in nt:
95,251
Flanking exon/introns:
chr1:37074949-37081375|chr1:37084289-37090797
Splice length:
346
Other database IDs:
circAtlas: mmu-Vwa3b_0011
Human Ortholog IDs:
Mature spliced Sequence:
ATTATGTGACTTCTTTGAGGAGACTCGTGGCTTCTCGGTATGCTGATGGCCTGTTCCCACAGATCTACACAGCAGAAGATGGCAGACTATACAACCTGACAGCAAAGACAGAGCTGATCTGCCAGTTTGTGGATTACTTAACACAAGCCGTGGAGAGCTACAAGCAGCGGATGGACTGGCTCACCAGCGGAAGCCGGCAGATTTTTGGTGTTATCTTGGAACAGAGAGTCACCATAGTGCTGGACTTCGGTGACGTTCTGATGGAGGAGCTCAATCTGTGTCGGGATGCTTTGAAAATGGTCCTCCAGGAGCAGGTGGCTCTCATCACCAGGTTCAACATGATATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGACGTTCTGATGGAGGAGC
59.47
GGCCATCAGCATACCGAGAA
59.89
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGACGTTCTGATGGAGGAGC
59.47
TCAGCATACCGAGAAGCCAC
59.82
142
PCR template sequence:
TCGGTGACGTTCTGATGGAGGAGCTCAATCTGTGTCGGGATGCTTTGAAAATGGTCCTCCAGGAGCAGGTGGCTCTCATCACCAGGTTCAACATGATATGATTATGTGACTTCTTTGAGGAGACTCGTGGCTTCTCGGTATGCTGATGGCCTGTTCCCACAGATCTACACAGCAGAAGATGGCAGACTATACAACCTGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
TCAACATGATATGATTATGTGACTTC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCVWA3B_346_ORF_1
1.1611
0.340154074
0.393642899
177
ORF sequence:
ATGGACTGGCTCACCAGCGGAAGCCGGCAGATTTTTGGTGTTATCTTGGAACAGAGAGTCACCATAGTGCTGGACTTCGGTGACGTTCTGATGGAGGAGCTCAATCTGTGTCGGGATGCTTTGAAAATGGTCCTCCAGGAGCAGGTGGCTCTCATCACCAGGTTCAACATGATATGA
Peptide sequence:
MDWLTSGSRQIFGVILEQRVTIVLDFGDVLMEELNLCRDALKMVLQEQVALITRFNMI
IRESfinder result
IRESfinder score
0.451712766
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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