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mmu_circVps13a_567_2
Basic information of circular RNA
PanCircBase ID:
mmu_circVps13a_567_2
mm39_Chr. Position ID:
chr19|16726889|16737400|-
Gene name:
Vps13a
Transcript:
ENSMUST00000068156.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
10,9,8,7,6,5,4
Exon/intron offsets:
0,321,596,2593,4726,10061,10415
Genomic length:
10511
Exon/intron sizes in nt:
58,81,60,60,110,102,96
Flanking exon/introns:
chr19:16723442-16726889|chr19:16737400-16739512
Splice length:
567
Other database IDs:
CIRCpedia: MMU_CIRCpedia_11696, circAtlas: mmu-Vps13a_0003
circAtlas: hsa-VPS13A_0001, CIRCpedia: HSA_CIRCpedia_372479
Human Ortholog IDs:
Mature spliced Sequence:
GTAGTCTTAAACTTAAAATTCCATGGAAAAACCTTTATACTCAACCAGTTGAAGCTGTTTTGGAAGAAATTTTTTTACTCATAGTGCCTTCTTCTAGAATACAGTATGATCCTATCAAAGAAGAAAAGCAACTCATGGAAACAAAGCAACAGGAACTAAAAAGAATAGAAAAAGCAAAACAGAAAGTATTTGATAAAGAAAAGCCTCGGGAGGAGAAACAGGACACGTTTACAGAAAAGTTAGTTACTCAGATCATACAGAACCTTCAAGTGCAAATCTCCAGCATCCATATTCGTTATGAAGACGATATCACAAATGGAGACAAACCACTCTCTTTTGGTATTTCCCTTCAAAATATCAGCCTTCAGACAACTGATCAATACTGGATCCCATGTTTACATGATAATACTGAAAAGCTAGTCCGGAAGCTAATCCGATTAGATAATCTCTTTGCTTACTGGAATGTGAACTCTGAGATGTTTTATCTGAATGATTACGATGAATCGCTGAAAGCCTTGAAAAATGGTATTGTGAATGAAAATATTGTTCCAGAAGGTTATGATTTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATCTGAATGATTACGATGAATCGCTG
59.91
TCCAAAACAGCTTCAACTGGT
58.27
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATCTGAATGATTACGATGAATCGCT
58.84
TCCAAAACAGCTTCAACTGGT
58.27
148
PCR template sequence:
AACTCTGAGATGTTTTATCTGAATGATTACGATGAATCGCTGAAAGCCTTGAAAAATGGTATTGTGAATGAAAATATTGTTCCAGAAGGTTATGATTTTGGTAGTCTTAAACTTAAAATTCCATGGAAAAACCTTTATACTCAACCAGTTGAAGCTGTTTTGGAAGAAATTTTTTTACTCATAGTGCCTTCTTCTAGAAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-140-5p(1), mmu-miR-23a-3p(1), mmu-miR-136-5p(1), mmu-miR-382-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGTTATGATTTTGGTAGTCTTAAACT
siRNA sets
TTAAGACTACCAAAATCAT, TTTAAGACTACCAAAATCA, GACTACCAAAATCATAACC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCVPS13A_567_2_ORF_1
1.0958
0.120067057
0.988662119
999
ORF sequence:
ATGGAAACAAAGCAACAGGAACTAAAAAGAATAGAAAAAGCAAAACAGAAAGTATTTGATAAAGAAAAGCCTCGGGAGGAGAAACAGGACACGTTTACAGAAAAGTTAGTTACTCAGATCATACAGAACCTTCAAGTGCAAATCTCCAGCATCCATATTCGTTATGAAGACGATATCACAAATGGAGACAAACCACTCTCTTTTGGTATTTCCCTTCAAAATATCAGCCTTCAGACAACTGATCAATACTGGATCCCATGTTTACATGATAATACTGAAAAGCTAGTCCGGAAGCTAATCCGATTAGATAATCTCTTTGCTTACTGGAATGTGAACTCTGAGATGTTTTATCTGAATGATTACGATGAATCGCTGAAAGCCTTGAAAAATGGTATTGTGAATGAAAATATTGTTCCAGAAGGTTATGATTTTGGTAGTCTTAAACTTAAAATTCCATGGAAAAACCTTTATACTCAACCAGTTGAAGCTGTTTTGGAAGAAATTTTTTTACTCATAGTGCCTTCTTCTAGAATACAGTATGATCCTATCAAAGAAGAAAAGCAACTCATGGAAACAAAGCAACAGGAACTAAAAAGAATAGAAAAAGCAAAACAGAAAGTATTTGATAAAGAAAAGCCTCGGGAGGAGAAACAGGACACGTTTACAGAAAAGTTAGTTACTCAGATCATACAGAACCTTCAAGTGCAAATCTCCAGCATCCATATTCGTTATGAAGACGATATCACAAATGGAGACAAACCACTCTCTTTTGGTATTTCCCTTCAAAATATCAGCCTTCAGACAACTGATCAATACTGGATCCCATGTTTACATGATAATACTGAAAAGCTAGTCCGGAAGCTAATCCGATTAGATAATCTCTTTGCTTACTGGAATGTGAACTCTGAGATGTTTTATCTGAATGATTACGATGAATCGCTGAAAGCCTTGAAAAATGGTATTGTGAATGAAAATATTGTTCCAGAAGGTTATGATTTT
Peptide sequence:
METKQQELKRIEKAKQKVFDKEKPREEKQDTFTEKLVTQIIQNLQVQISSIHIRYEDDITNGDKPLSFGISLQNISLQTTDQYWIPCLHDNTEKLVRKLIRLDNLFAYWNVNSEMFYLNDYDESLKALKNGIVNENIVPEGYDFGSLKLKIPWKNLYTQPVEAVLEEIFLLIVPSSRIQYDPIKEEKQLMETKQQELKRIEKAKQKVFDKEKPREEKQDTFTEKLVTQIIQNLQVQISSIHIRYEDDITNGDKPLSFGISLQNISLQTTDQYWIPCLHDNTEKLVRKLIRLDNLFAYWNVNSEMFYLNDYDESLKALKNGIVNENIVPEGYDF
IRESfinder result
IRESfinder score
0.779481304
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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