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mmu_circUty_457
Basic information of circular RNA
PanCircBase ID:
mmu_circUty_457
mm39_Chr. Position ID:
chrY|1102764|1131223|-
Gene name:
Uty
Transcript:
ENSMUST00000143286.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
23,22,21
Exon/intron offsets:
0,27105,28271
Genomic length:
28459
Exon/intron sizes in nt:
12,71,42,188
Flanking exon/introns:
chrY:1099752-1102764|chrY:1131223-1134871
Splice length:
457
Other database IDs:
CIRCpedia: MMU_CIRCpedia_2835, circAtlas: mmu-Uty_0014
Human Ortholog IDs:
Mature spliced Sequence:
AAATAATTTGAATTTCCTGATGAGTTCTTGGTGGCCGAACCTTGAAGATCTTTATGAAGCAAATGTTCCTGTTTATAGGTTTATTCAACGACCTGGTGATTTGGTTTGGATAAATGCAGGCACTGTTCACTGGGTTCAGGCTATTGGGTGGTGTAACAACATCACCTGGAATGTTGGTCCACTCACAGCCTTCCAATATAAATTGGCAGTTGAACGATATGAATGGAATAAATTGCAAAGTGTCAAGTCAGTAGTACCCATGGTTCATCTTTCATGGAATATGGCACGAAATATCAAAGTCTCAGATCCAAAGCTTTTTGAAATGATTAAATATTGTCTCCTCAAGATTCTAAAGCATTGTCAGACTTTGAGAGAAGCTCTTGTTGCAGCAGGGAAAGAGGTTTTGTGGCATGGGAGGATAAATGATGAACCAGCTCCTTACTGTAGCATCTGTGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGAGAAGCTCTTGTTGCAGC
59.54
AAGATCTTCAAGGTTCGGCCA
59.65
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCAGCAGGGAAAGAGGTTT
59.81
TCACCAGGTCGTTGAATAAACCT
59.93
172
PCR template sequence:
TTGTCAGACTTTGAGAGAAGCTCTTGTTGCAGCAGGGAAAGAGGTTTTGTGGCATGGGAGGATAAATGATGAACCAGCTCCTTACTGTAGCATCTGTGAGAAATAATTTGAATTTCCTGATGAGTTCTTGGTGGCCGAACCTTGAAGATCTTTATGAAGCAAATGTTCCTGTTTATAGGTTTATTCAACGACCTGGTGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-370-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TAGCATCTGTGAGAAATAATTTGAAT
siRNA sets
ATTCAAATTATTTCTCACA, AATTATTTCTCACAGATGC, TTCAAATTATTTCTCACAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUTY_457_ORF_1
0.9013
-0.104300346
0.385946654
492
ORF sequence:
ATGATGAACCAGCTCCTTACTGTAGCATCTGTGAGAAATAATTTGAATTTCCTGATGAGTTCTTGGTGGCCGAACCTTGAAGATCTTTATGAAGCAAATGTTCCTGTTTATAGGTTTATTCAACGACCTGGTGATTTGGTTTGGATAAATGCAGGCACTGTTCACTGGGTTCAGGCTATTGGGTGGTGTAACAACATCACCTGGAATGTTGGTCCACTCACAGCCTTCCAATATAAATTGGCAGTTGAACGATATGAATGGAATAAATTGCAAAGTGTCAAGTCAGTAGTACCCATGGTTCATCTTTCATGGAATATGGCACGAAATATCAAAGTCTCAGATCCAAAGCTTTTTGAAATGATTAAATATTGTCTCCTCAAGATTCTAAAGCATTGTCAGACTTTGAGAGAAGCTCTTGTTGCAGCAGGGAAAGAGGTTTTGTGGCATGGGAGGATAAATGATGAACCAGCTCCTTACTGTAGCATCTGTGAG
Peptide sequence:
MMNQLLTVASVRNNLNFLMSSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNITWNVGPLTAFQYKLAVERYEWNKLQSVKSVVPMVHLSWNMARNIKVSDPKLFEMIKYCLLKILKHCQTLREALVAAGKEVLWHGRINDEPAPYCSICE
IRESfinder result
IRESfinder score
0.798265178
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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