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mmu_circUty_362
Basic information of circular RNA
PanCircBase ID:
mmu_circUty_362
mm39_Chr. Position ID:
chrY|1197205|1226826|-
Gene name:
Uty
Transcript:
ENSMUST00000154666.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
7,6,5,4
Exon/intron offsets:
0,2249,27291,29489
Genomic length:
29621
Exon/intron sizes in nt:
1,21,59,50,132
Flanking exon/introns:
chrY:1189390-1197205|chrY:1226826-1239710
Splice length:
362
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTCAGAGGTTCAGTCCATTATCATCAAGGTGGGAGCATGGCAGTATCCAGGAAGGCATGGCACAGGAGGAGCTGAGAGTTCTGTCTTCATCCAAAGGCAGCTAGTGGAAGACTGACTTCCAGGCAACTAAGCATTGTCATCTTACCAGAGATACTACAGTTTACAGACTGACTACTGGAAGAATGCTGCCTTTTTATATGGTCTTGGTTTGGTCTATTTCTACTACAATGCATTTCAGTGGGCAATTAGAGCTTTTCAGGAAGTCCTTTATGTTGACCCTAATTTTTGCAGAGCCAAAGAAATCCATTTACGACTTGGTTTTATGTTCAAAATGAATACAGATTATGAATCTAGTTTAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTGCAGAGCCAAAGAAATCCA
58.4
GTGCCATGCCTTCCTGGATA
59.82
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACCCTAATTTTTGCAGAGCCA
59.17
TGCCATGCCTTCCTGGATAC
59.82
150
PCR template sequence:
AGTCCTTTATGTTGACCCTAATTTTTGCAGAGCCAAAGAAATCCATTTACGACTTGGTTTTATGTTCAAAATGAATACAGATTATGAATCTAGTTTAAAGGTTCAGAGGTTCAGTCCATTATCATCAAGGTGGGAGCATGGCAGTATCCAGGAAGGCATGGCACAGGAGGAGCTGAGAGTTCTGTCTTCATCCAAAGGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-33-5p(1), mmu-miR-22-3p(1), mmu-miR-107-3p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
ATCTAGTTTAAAGGTTCAGAGGTTCA
siRNA sets
AACCTCTGAACCTTTAAAC, TCTGAACCTTTAAACTAGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUTY_362_ORF_3
0.4931
0.027214336
0.039442477
144
ORF sequence:
ATGTTCAAAATGAATACAGATTATGAATCTAGTTTAAAGGTTCAGAGGTTCAGTCCATTATCATCAAGGTGGGAGCATGGCAGTATCCAGGAAGGCATGGCACAGGAGGAGCTGAGAGTTCTGTCTTCATCCAAAGGCAGCTAG
Peptide sequence:
MFKMNTDYESSLKVQRFSPLSSRWEHGSIQEGMAQEELRVLSSSKGS
IRESfinder result
IRESfinder score
0.787555968
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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