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mmu_circUtrn_781
Basic information of circular RNA
PanCircBase ID:
mmu_circUtrn_781
mm39_Chr. Position ID:
chr10|12587181|12596686|-
Gene name:
Utrn
Transcript:
ENSMUST00000076817.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
21,20,19,18,17
Exon/intron offsets:
0,1816,2695,8580,9381
Genomic length:
9505
Exon/intron sizes in nt:
146,181,242,88,124
Flanking exon/introns:
chr10:12585922-12587181|chr10:12596686-12601954
Splice length:
781
Other database IDs:
circAtlas: mmu-Utrn_0123
Human Ortholog IDs:
Mature spliced Sequence:
GTTTGATGCTATAAGTACAGAGCTGCTGAACTGGATTTTGAAATCAAAGACTGCCATTCAGAACACAGAGATGAAAGAATATAAGAAGTCGCAGGAGACCTCAGGAATGAAAAAGAAATTGAAGGGATTAGAGAAAGAACAGAAGGAAAATCTGCCCCGACTGGACGAACTGAATCAAACCGGACAAACCCTCCGGGAGCAAATGGGAAAAGAAGGCCTTTCCACTGAAGAAGTAAACGATGTTCTGGAAAGGGTTTCGTTGGAGTGGAAGATGATATCTCAGCAGCTAGAAGATCTGGGAAGGAAGATCCAGCTGCAGGAAGATATAAATGCTTATTTTAAGCAGCTTGATGCCATTGAGGAGACCATCAAGGAGAAGGAAGAGTGGCTGAGGGGCACACCCATTTCTGAATCGCCCCGGCAGCCCTTGCCAGGCTTAAAGGATTCTTGCCAGAGGGAACTGACAGATCTCCTTGGCCTTCACCCCAGAATTGAGACGCTGTGTGCAAGCTGTTCAGCCCTGAAGTCTCAGCCCTGTGTCCCAGGTTTTGTCCAGCAGGGTTTTGACGACCTTCGACATCATTACCAGGCTGTGCGGAAGGCTTTAGAGGAATACCAACAACAACTAGAAAATGAGCTGAAGAGCCAGCCTGGACCCGCGTATTTGGACACACTGAATACCCTGAAAAAAATGCTAAGCGAGTCAGAAAAGGCGGCCCAGGCCTCTCTGAATGCCCTGAACGATCCCATAGCGGTGGAGCAGGCCCTGCAGGAGAAAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGCGAGTCAGAAAAGGCGG
60.95
TCTGTGTTCTGAATGGCAGTCT
59.63
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGCGAGTCAGAAAAGGCGG
60.95
TCTCTGTGTTCTGAATGGCAGT
59.63
156
PCR template sequence:
CCTGAAAAAAATGCTAAGCGAGTCAGAAAAGGCGGCCCAGGCCTCTCTGAATGCCCTGAACGATCCCATAGCGGTGGAGCAGGCCCTGCAGGAGAAAAAGGTTTGATGCTATAAGTACAGAGCTGCTGAACTGGATTTTGAAATCAAAGACTGCCATTCAGAACACAGAGATGAAAGAATATAAGAAGTCGCAGGAGACC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-10b-5p(1), mmu-miR-370-3p(1), mmu-miR-207(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCAGGAGAAAAAGGTTTGATGCTATA
siRNA sets
TCAAACCTTTTTCTCCTGC, ATCAAACCTTTTTCTCCTG, ATAGCATCAAACCTTTTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUTRN_781_ORF_1
1.1387
0.30041301
0.962323953
717
ORF sequence:
ATGAAAGAATATAAGAAGTCGCAGGAGACCTCAGGAATGAAAAAGAAATTGAAGGGATTAGAGAAAGAACAGAAGGAAAATCTGCCCCGACTGGACGAACTGAATCAAACCGGACAAACCCTCCGGGAGCAAATGGGAAAAGAAGGCCTTTCCACTGAAGAAGTAAACGATGTTCTGGAAAGGGTTTCGTTGGAGTGGAAGATGATATCTCAGCAGCTAGAAGATCTGGGAAGGAAGATCCAGCTGCAGGAAGATATAAATGCTTATTTTAAGCAGCTTGATGCCATTGAGGAGACCATCAAGGAGAAGGAAGAGTGGCTGAGGGGCACACCCATTTCTGAATCGCCCCGGCAGCCCTTGCCAGGCTTAAAGGATTCTTGCCAGAGGGAACTGACAGATCTCCTTGGCCTTCACCCCAGAATTGAGACGCTGTGTGCAAGCTGTTCAGCCCTGAAGTCTCAGCCCTGTGTCCCAGGTTTTGTCCAGCAGGGTTTTGACGACCTTCGACATCATTACCAGGCTGTGCGGAAGGCTTTAGAGGAATACCAACAACAACTAGAAAATGAGCTGAAGAGCCAGCCTGGACCCGCGTATTTGGACACACTGAATACCCTGAAAAAAATGCTAAGCGAGTCAGAAAAGGCGGCCCAGGCCTCTCTGAATGCCCTGAACGATCCCATAGCGGTGGAGCAGGCCCTGCAGGAGAAAAAGGTTTGA
Peptide sequence:
MKEYKKSQETSGMKKKLKGLEKEQKENLPRLDELNQTGQTLREQMGKEGLSTEEVNDVLERVSLEWKMISQQLEDLGRKIQLQEDINAYFKQLDAIEETIKEKEEWLRGTPISESPRQPLPGLKDSCQRELTDLLGLHPRIETLCASCSALKSQPCVPGFVQQGFDDLRHHYQAVRKALEEYQQQLENELKSQPGPAYLDTLNTLKKMLSESEKAAQASLNALNDPIAVEQALQEKKV
IRESfinder result
IRESfinder score
0.448049113
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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