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mmu_circUtp25_491
Basic information of circular RNA
PanCircBase ID:
mmu_circUtp25_491
mm39_Chr. Position ID:
chr1|192805945|192811838|-
Gene name:
Utp25
Transcript:
ENSMUST00000085555.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
4,3,2
Exon/intron offsets:
0,4629,5853
Genomic length:
5893
Exon/intron sizes in nt:
171,280,40
Flanking exon/introns:
chr1:192804402-192805945|chr1:192811838-192812351
Splice length:
491
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGTTTCCAAGAAGGAAGCCAAGCCGCAGATTTGTCAGCTGCCAGAGAGTTCAGATTCTTCACATTCTGAAAGTGAATCAGAGAGTGAACAAGAGCACGTTTCAGGGTACCACAGACTGCTTGCTACATTAAAGAATGTTTCTGAGGAAGAGGAAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGAGGAAGAGGAAGATGACAGTGCTGTAGGTGATGCAGAAATGAATGAGGAAGCTGGCAGTGAGGATGGCAGTGTGGGAGAAGCTGCTGTCTCGGAGGCTGCTGAGGAGGCTGCTGAGACACAGGAACATATGTCTCTAGCTGACAACTCAAAAGAAAAAGATGGTGAAGAGCCACCTGGTGTATCACAGAAATCCTCAGAGGAGTTCACAGATGTGAAACACGAGTCACTGTTCAGCCTGGAAACTAATTTTCTAGAAGAGGATAGCGGGGGCAGTTGTTCCCAGAGGCCATCCCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACGAGTCACTGTTCAGCCTG
59.97
ACGTGCTCTTGTTCACTCTCT
59.32
177
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CACGAGTCACTGTTCAGCCT
59.97
ACGTGCTCTTGTTCACTCTCT
59.32
178
PCR template sequence:
GAGGAGTTCACAGATGTGAAACACGAGTCACTGTTCAGCCTGGAAACTAATTTTCTAGAAGAGGATAGCGGGGGCAGTTGTTCCCAGAGGCCATCCCAAGGGTTTCCAAGAAGGAAGCCAAGCCGCAGATTTGTCAGCTGCCAGAGAGTTCAGATTCTTCACATTCTGAAAGTGAATCAGAGAGTGAACAAGAGCACGTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-207(2), mmu-miR-101b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGGCCATCCCAAGGGTTTCCAAGAAG
siRNA sets
CTTGGAAACCCTTGGGATG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUTP25_491_ORF_1
0.9658
0.187345034
0.527549714
390
ORF sequence:
ATGAATGAGGAAGCTGGCAGTGAGGATGGCAGTGTGGGAGAAGCTGCTGTCTCGGAGGCTGCTGAGGAGGCTGCTGAGACACAGGAACATATGTCTCTAGCTGACAACTCAAAAGAAAAAGATGGTGAAGAGCCACCTGGTGTATCACAGAAATCCTCAGAGGAGTTCACAGATGTGAAACACGAGTCACTGTTCAGCCTGGAAACTAATTTTCTAGAAGAGGATAGCGGGGGCAGTTGTTCCCAGAGGCCATCCCAAGGGTTTCCAAGAAGGAAGCCAAGCCGCAGATTTGTCAGCTGCCAGAGAGTTCAGATTCTTCACATTCTGAAAGTGAATCAGAGAGTGAACAAGAGCACGTTTCAGGGTACCACAGACTGCTTGCTACATTAA
Peptide sequence:
MNEEAGSEDGSVGEAAVSEAAEEAAETQEHMSLADNSKEKDGEEPPGVSQKSSEEFTDVKHESLFSLETNFLEEDSGGSCSQRPSQGFPRRKPSRRFVSCQRVQILHILKVNQRVNKSTFQGTTDCLLH
IRESfinder result
IRESfinder score
0.356787158
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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