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mmu_circUsp34_3289
Basic information of circular RNA
PanCircBase ID:
mmu_circUsp34_3289
mm39_Chr. Position ID:
chr11|23304844|23351578|+
Gene name:
Usp34
Transcript:
ENSMUST00000180046.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
22
Exon/intron indexes:
9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30
Exon/intron offsets:
0,161,6434,6742,7732,8213,8946,12494,15394,15599,18476,20156,22710,26380,27709,29556,30168,33589,33819,37997,38907,46608
Genomic length:
46734
Exon/intron sizes in nt:
8,29,31,26,13,03,61,31,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr11:23301653-23304844|chr11:23351578-23353559
Splice length:
3289
Other database IDs:
circAtlas: mmu-Usp34_0141
Human Ortholog IDs:
Mature spliced Sequence:
GTCCATTGCAAAAGAACTTGCAGACTGGCTTATTAGCAACAATGTAGTAGAACATATATTTGGACCAAATTTACATATTGAGATTATTAAACAGTGCCAAGTGATTTTGAATTTCTTGGCAGCAGAGGGACGACTGAGTACTCAGCATATTGACTGCATTTGGGCTGCAGCACAGCTGAAGCACTGTAGTCGATATATTCATGACTTATTCCCTTCACTCATCAAGAATCTGGATCCTGTGCCACTTAGACATTTACTTAATCTGGTCTCAGCTCTTGAACCAGGTGTTCATACTGAACAGACACTGTACTTGGCATCCATGTTAATTAAAGCTTTGTGGAATAATGCACTTGCAGCTAAGGCTCAGCTGTCTAAACAGAGTTCTTTTGCATCTTTATTAAACACTAATATGCCCATTGGAAACAAGAAAGAGGAAGAAGAGCTTAGAAGAGCAGCTCCTTCACCTTGGTCCCCAGCAGCGAGTCCTCAAAGTAGTGATAACAGTGACACACATCAGAGTGGAGCCAGTGACATTGAAATGGATGAGCAGCTTATTAATAGAAATAAACATGTGCAGCAGCGACTGTCTGACACTGAGGAATCCATGCAAGGAAGTTCTGATGAAACTGCCAACAGTGGTGAAGATGGAAGCAGTGGCCCTGGTAGCAGTAGTGGGCATAGTGATGGATCTAGCAATGAAGTTAATTCAAGCCATGCAAGCCAGTCTGCTGGGAGCCCTGGAAGTGAGGTGCAATCAGAAGACATTGCAGACATCGAGGCCCTTAAAGAAGAAGAGGAAGAGGAGGAGGAAGAAGAAGAAGAGGAAGAGGAAGAAGATGATGAAGAAGAAGAAGATGAAGAAGAAGATGATGATGACGACGATGATCATGGTCACAATCCTGCCAAAAATACTTGTGGTACAGAACTTCGAAACAGAAAGTTAGAAAATCCAGCAGGCATTTGCTTGGGGGAATCCCAAGGCACATCTGAAAGGAATGGGACAAATAGTGGAACTGGAAAGGACCTGGTTTTTAATACTGAGCCATTGCCATCTGTAGATAACCGAATACGAATGCTAGATGCCTGTGCTCACTCTGAAGACCCAGAACATGGCATTTCAGGGGAAGTGAGTTCTGCTCACTTAGCACAAGGTTCTCAGGAGGCTTGTATCACACGAAGTGGGGACTTCCTTGGGGAGACTATTGGGAATGAGTTGTTTAATTGTCGGCAGTTTATTGGTCCACAGCATCACCACCACCACCACCACCACCACCACCATCATCACCATCACCACCACCACCACCACCACCACCACGATGGACATATGGTTGATGATATGCTAAGTGCAGATGATGTCAGTTGCAGTAGCTCCCAGGTCAGTGCAAAATCAGAAAAAAATATGGCTGATTTTGATGGTGAAGAATCTGGATGTGAAGAGGAACTAGTTCAGATAAATTCACATGCAGAACTAACATCTCATCTCCAACAACATCTTCCCAATTTAGCATCTATTTATCATGAACATCTTAGTCAAGGACCTGCAGTTCATAAACATCAATTCAGTAGTAATGCTGTGACAGATATTAATTTGGATAATGTTTGCAAGAAAGGAAATACTTTATTATGGGATATAGTGCAAGATGATGATGCAATTAATCTTTCTGAAGGATTAATAAATGAAGCAGAGAAACTTCTCTGTTCTTTGGTTTGTTGGTTTACGGATAGACAAATTCGAATGAGATTCATTGAAGGCTGCCTTGAAAATTTAGGGAACAACAGATCAGTGGTGATTTCACTTCGTCTTCTTCCAAAACTGTTTGGTACTTTTCAGCAGTTTGGAAGCAGTTATGATACTCATTGGATTACAATGTGGGCAGAGAAAGAACTGAACATGATGAAGCTTTTCTTTGACAATCTGGTGTATTACATTCAAGGTATAAGGGAAGGCAGACAAAAGCATGCACTGTACAGCCATAGTGCCGAAGTTCAAGTTCGACTTCAGTTCCTGACCTGTGTGTTTTCAACCCTGGGATCCCCTGATCACTTCAGGTTAAGTTTAGAGCAAGTGGACATTTTATGGCATTGTTTGGTAGAAGACTCTGAGTGCTATGATGATGCACTTCATTGGTTTTTAAATCAAGTTCGAAGTAAAGATCAACATGCAATGGGAATGGAAACCTATAAACATCTTTTCCTGGAGAAGATGCCCCAGCTTAAACCTGAAACAATTAGCATGACTGGCTTAAACCTGTTTCAGCATCTTTGTAACTTGGCTCGATTGGCTACCAGTGCCTATGATGGTGGCTCAAACTCTGAGCTGTGTGGTATGGACCAATTTTGGGGTATTGCATTAAGAGCACAATCAGGTGATGTCAGTAGAGCTGCTATTCAGTATATTAACTCCTACTATATTAATGGTAAAACGGGTTTGGAAAAGGAGCAAGAATTTATTAGTAAGTGCATGGAAAGTCTCATGATAGCTTCTAGCAGTCTTGAACAAGAATCACACTCAAGTCTCACAGTTATAGAACGAGGATTGCTTATGCTGAAGACACATCTGGAAGCATTTAGGAGAAGGTTTGCATATCATCTGAGACAGTGGCAGATTGAGGGCACCGGTATTAGCAGTCACTTGAAAGCACTGAGTGACAAACAGTCTTTGCCTCTAAGGGTTGTATGTCAGCCTGCTGGACTTCCCGACAAGATGACTATTGAAATGTATCCAAGTGACCAGGTAGCAGATCTTCGTGCTGAAGTAACTCACTGGTATGAAAACTTACAGAAAGAACAAATAAATCAACAAGCTCAGCTTCAGGAGTTTGGTCAAAGCAGCAGAAAGGGAGAGTTTCCTGGTGGCCTCATGGGGCCTGTCAGGATGATCTCCTCTGGACATGAATTAACAACAGACTATGATGAAAAAGCACTTCATGAACTTGGTTTTAAAGATATGCAGATGGTGTTTGTATCTTTGGGTGCACCAAGAAGAGAAAGAAAAGGAGAAGGGGTTCAACTGCCTGCATCTTGCCTCCCACCACCTCAGAAGGACAACATTCCGATGCTTTTGCTGTTACAAGAACCTCATTTAACTACTCTCTTTGATTTATTAGAAATGCTTGCTTCATTTAAACCACCCTCAGGAAAAGTTGCAGTTGATGACAGTGAGAGTTTAAAATGTGAAGAACTTCATCTTCATGCAGAAAATCTTTCAAGGCGTGTCTGGGAGTTGCTGATGCTTCTTCCGACTTGTCCCAACATGCTGACGGCATTCCAGAATGTCTCAGACGAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTTCTTCCGACTTGTCCCA
59.97
TTGGCACTGTTTAATAATCTCAAT
55.08
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTTCTTCCGACTTGTCCC
59.97
TTGGCACTGTTTAATAATCTCAAT
55.08
159
PCR template sequence:
TCATGCAGAAAATCTTTCAAGGCGTGTCTGGGAGTTGCTGATGCTTCTTCCGACTTGTCCCAACATGCTGACGGCATTCCAGAATGTCTCAGACGAGCAGGTCCATTGCAAAAGAACTTGCAGACTGGCTTATTAGCAACAATGTAGTAGAACATATATTTGGACCAAATTTACATATTGAGATTATTAAACAGTGCCAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
24
Interacting miRNAs (#binding sites)
mmu-miR-207(2), mmu-miR-134-5p(1), mmu-miR-32-5p(1), mmu-miR-221-3p(1), mmu-miR-31-5p(1), mmu-miR-92a-3p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-29a-3p(1), mmu-miR-152-3p(1), mmu-miR-25-3p(1), mmu-miR-139-5p(1), mmu-miR-214-3p(1), mmu-miR-24-3p(1), mmu-miR-149-5p(1), mmu-miR-22-3p(1), mmu-miR-194-5p(1), mmu-miR-758-3p(1), mmu-miR-705(1), mmu-miR-466a-5p(1), mmu-miR-466p-5p(1), mmu-miR-335-3p(1), mmu-miR-532-5p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
CTCAGACGAGCAGGTCCATTGCAAAA
siRNA sets
TTTGCAATGGACCTGCTCG, TTTTGCAATGGACCTGCTC, TTGCAATGGACCTGCTCGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUSP34_3289_ORF_1
1.1055
0.043938335
0.999999999
3306
ORF sequence:
ATGTCTCAGACGAGCAGGTCCATTGCAAAAGAACTTGCAGACTGGCTTATTAGCAACAATGTAGTAGAACATATATTTGGACCAAATTTACATATTGAGATTATTAAACAGTGCCAAGTGATTTTGAATTTCTTGGCAGCAGAGGGACGACTGAGTACTCAGCATATTGACTGCATTTGGGCTGCAGCACAGCTGAAGCACTGTAGTCGATATATTCATGACTTATTCCCTTCACTCATCAAGAATCTGGATCCTGTGCCACTTAGACATTTACTTAATCTGGTCTCAGCTCTTGAACCAGGTGTTCATACTGAACAGACACTGTACTTGGCATCCATGTTAATTAAAGCTTTGTGGAATAATGCACTTGCAGCTAAGGCTCAGCTGTCTAAACAGAGTTCTTTTGCATCTTTATTAAACACTAATATGCCCATTGGAAACAAGAAAGAGGAAGAAGAGCTTAGAAGAGCAGCTCCTTCACCTTGGTCCCCAGCAGCGAGTCCTCAAAGTAGTGATAACAGTGACACACATCAGAGTGGAGCCAGTGACATTGAAATGGATGAGCAGCTTATTAATAGAAATAAACATGTGCAGCAGCGACTGTCTGACACTGAGGAATCCATGCAAGGAAGTTCTGATGAAACTGCCAACAGTGGTGAAGATGGAAGCAGTGGCCCTGGTAGCAGTAGTGGGCATAGTGATGGATCTAGCAATGAAGTTAATTCAAGCCATGCAAGCCAGTCTGCTGGGAGCCCTGGAAGTGAGGTGCAATCAGAAGACATTGCAGACATCGAGGCCCTTAAAGAAGAAGAGGAAGAGGAGGAGGAAGAAGAAGAAGAGGAAGAGGAAGAAGATGATGAAGAAGAAGAAGATGAAGAAGAAGATGATGATGACGACGATGATCATGGTCACAATCCTGCCAAAAATACTTGTGGTACAGAACTTCGAAACAGAAAGTTAGAAAATCCAGCAGGCATTTGCTTGGGGGAATCCCAAGGCACATCTGAAAGGAATGGGACAAATAGTGGAACTGGAAAGGACCTGGTTTTTAATACTGAGCCATTGCCATCTGTAGATAACCGAATACGAATGCTAGATGCCTGTGCTCACTCTGAAGACCCAGAACATGGCATTTCAGGGGAAGTGAGTTCTGCTCACTTAGCACAAGGTTCTCAGGAGGCTTGTATCACACGAAGTGGGGACTTCCTTGGGGAGACTATTGGGAATGAGTTGTTTAATTGTCGGCAGTTTATTGGTCCACAGCATCACCACCACCACCACCACCACCACCACCATCATCACCATCACCACCACCACCACCACCACCACCACGATGGACATATGGTTGATGATATGCTAAGTGCAGATGATGTCAGTTGCAGTAGCTCCCAGGTCAGTGCAAAATCAGAAAAAAATATGGCTGATTTTGATGGTGAAGAATCTGGATGTGAAGAGGAACTAGTTCAGATAAATTCACATGCAGAACTAACATCTCATCTCCAACAACATCTTCCCAATTTAGCATCTATTTATCATGAACATCTTAGTCAAGGACCTGCAGTTCATAAACATCAATTCAGTAGTAATGCTGTGACAGATATTAATTTGGATAATGTTTGCAAGAAAGGAAATACTTTATTATGGGATATAGTGCAAGATGATGATGCAATTAATCTTTCTGAAGGATTAATAAATGAAGCAGAGAAACTTCTCTGTTCTTTGGTTTGTTGGTTTACGGATAGACAAATTCGAATGAGATTCATTGAAGGCTGCCTTGAAAATTTAGGGAACAACAGATCAGTGGTGATTTCACTTCGTCTTCTTCCAAAACTGTTTGGTACTTTTCAGCAGTTTGGAAGCAGTTATGATACTCATTGGATTACAATGTGGGCAGAGAAAGAACTGAACATGATGAAGCTTTTCTTTGACAATCTGGTGTATTACATTCAAGGTATAAGGGAAGGCAGACAAAAGCATGCACTGTACAGCCATAGTGCCGAAGTTCAAGTTCGACTTCAGTTCCTGACCTGTGTGTTTTCAACCCTGGGATCCCCTGATCACTTCAGGTTAAGTTTAGAGCAAGTGGACATTTTATGGCATTGTTTGGTAGAAGACTCTGAGTGCTATGATGATGCACTTCATTGGTTTTTAAATCAAGTTCGAAGTAAAGATCAACATGCAATGGGAATGGAAACCTATAAACATCTTTTCCTGGAGAAGATGCCCCAGCTTAAACCTGAAACAATTAGCATGACTGGCTTAAACCTGTTTCAGCATCTTTGTAACTTGGCTCGATTGGCTACCAGTGCCTATGATGGTGGCTCAAACTCTGAGCTGTGTGGTATGGACCAATTTTGGGGTATTGCATTAAGAGCACAATCAGGTGATGTCAGTAGAGCTGCTATTCAGTATATTAACTCCTACTATATTAATGGTAAAACGGGTTTGGAAAAGGAGCAAGAATTTATTAGTAAGTGCATGGAAAGTCTCATGATAGCTTCTAGCAGTCTTGAACAAGAATCACACTCAAGTCTCACAGTTATAGAACGAGGATTGCTTATGCTGAAGACACATCTGGAAGCATTTAGGAGAAGGTTTGCATATCATCTGAGACAGTGGCAGATTGAGGGCACCGGTATTAGCAGTCACTTGAAAGCACTGAGTGACAAACAGTCTTTGCCTCTAAGGGTTGTATGTCAGCCTGCTGGACTTCCCGACAAGATGACTATTGAAATGTATCCAAGTGACCAGGTAGCAGATCTTCGTGCTGAAGTAACTCACTGGTATGAAAACTTACAGAAAGAACAAATAAATCAACAAGCTCAGCTTCAGGAGTTTGGTCAAAGCAGCAGAAAGGGAGAGTTTCCTGGTGGCCTCATGGGGCCTGTCAGGATGATCTCCTCTGGACATGAATTAACAACAGACTATGATGAAAAAGCACTTCATGAACTTGGTTTTAAAGATATGCAGATGGTGTTTGTATCTTTGGGTGCACCAAGAAGAGAAAGAAAAGGAGAAGGGGTTCAACTGCCTGCATCTTGCCTCCCACCACCTCAGAAGGACAACATTCCGATGCTTTTGCTGTTACAAGAACCTCATTTAACTACTCTCTTTGATTTATTAGAAATGCTTGCTTCATTTAAACCACCCTCAGGAAAAGTTGCAGTTGATGACAGTGAGAGTTTAAAATGTGAAGAACTTCATCTTCATGCAGAAAATCTTTCAAGGCGTGTCTGGGAGTTGCTGATGCTTCTTCCGACTTGTCCCAACATGCTGACGGCATTCCAGAATGTCTCAGACGAGCAG
Peptide sequence:
MSQTSRSIAKELADWLISNNVVEHIFGPNLHIEIIKQCQVILNFLAAEGRLSTQHIDCIWAAAQLKHCSRYIHDLFPSLIKNLDPVPLRHLLNLVSALEPGVHTEQTLYLASMLIKALWNNALAAKAQLSKQSSFASLLNTNMPIGNKKEEEELRRAAPSPWSPAASPQSSDNSDTHQSGASDIEMDEQLINRNKHVQQRLSDTEESMQGSSDETANSGEDGSSGPGSSSGHSDGSSNEVNSSHASQSAGSPGSEVQSEDIADIEALKEEEEEEEEEEEEEEEEDDEEEEDEEEDDDDDDDHGHNPAKNTCGTELRNRKLENPAGICLGESQGTSERNGTNSGTGKDLVFNTEPLPSVDNRIRMLDACAHSEDPEHGISGEVSSAHLAQGSQEACITRSGDFLGETIGNELFNCRQFIGPQHHHHHHHHHHHHHHHHHHHHHHHDGHMVDDMLSADDVSCSSSQVSAKSEKNMADFDGEESGCEEELVQINSHAELTSHLQQHLPNLASIYHEHLSQGPAVHKHQFSSNAVTDINLDNVCKKGNTLLWDIVQDDDAINLSEGLINEAEKLLCSLVCWFTDRQIRMRFIEGCLENLGNNRSVVISLRLLPKLFGTFQQFGSSYDTHWITMWAEKELNMMKLFFDNLVYYIQGIREGRQKHALYSHSAEVQVRLQFLTCVFSTLGSPDHFRLSLEQVDILWHCLVEDSECYDDALHWFLNQVRSKDQHAMGMETYKHLFLEKMPQLKPETISMTGLNLFQHLCNLARLATSAYDGGSNSELCGMDQFWGIALRAQSGDVSRAAIQYINSYYINGKTGLEKEQEFISKCMESLMIASSSLEQESHSSLTVIERGLLMLKTHLEAFRRRFAYHLRQWQIEGTGISSHLKALSDKQSLPLRVVCQPAGLPDKMTIEMYPSDQVADLRAEVTHWYENLQKEQINQQAQLQEFGQSSRKGEFPGGLMGPVRMISSGHELTTDYDEKALHELGFKDMQMVFVSLGAPRRERKGEGVQLPASCLPPPQKDNIPMLLLLQEPHLTTLFDLLEMLASFKPPSGKVAVDDSESLKCEELHLHAENLSRRVWELLMLLPTCPNMLTAFQNVSDEQ
IRESfinder result
IRESfinder score
0.697398747
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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