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mmu_circUsp32_618
Basic information of circular RNA
PanCircBase ID:
mmu_circUsp32_618
mm39_Chr. Position ID:
chr11|84930820|84942100|-
Gene name:
Usp32
Transcript:
ENSMUST00000108075.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
14,13,12,11,10
Exon/intron offsets:
0,2493,4715,10392,11196
Genomic length:
11280
Exon/intron sizes in nt:
176,193,103,62,84
Flanking exon/introns:
chr11:84930167-84930820|chr11:84942100-84944473
Splice length:
618
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGGGCCATCTTACTCTTGAAGACTATCAGATATGGAGTGTGAAAAATGTTCTTGCCAATGAGTTTTTGAATCTCCTCTTCCAGGTATGCCACATAGTTCTGGGGTTAAGACCAGCTACTCCAGAAGAAGAAGGACAAATTATTAGAGGATGGTTGGAACGAGAAAGCAGGTATGGTCTGCAGCCAGGACACAATTGGTTTATCATCTCCATGCAGTGGTGGCAACAGTGGAAAGAATATGTCAAATATGATGCCAGCCCTGTTGTAATTGAGCCATCATCTGTTCTAAATGGAGGAAAATTTTCATTTGGAACAGCAGCACATCCTATAGAGCATGGAGAAGATAGAATCAGTAATAACCTTGGTTATATGAATACCACAGAAGAGAAATACTCAGACAACATTTCTTCTGCATCTGAAGCCTCAGAGTCTACTGGCAGTGGCTTTCTGTATTCTGGCACACCAGGGGCAGATATGTGCTTTGCAAGGCAGCATAACACTTCTGACAATAACAACCAGTGTTTACTAGGAGCCAATGGGAATATCCTTTTGCACCTTAATCCTCAGAAGCCAGGAGCAATTGATAACCAGCCACTAGTAACTCAGGAACCAGTAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AATCCTCAGAAGCCAGGAGC
59.45
TGGCATACCTGGAAGAGGAGA
59.99
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AATCCTCAGAAGCCAGGAGC
59.45
GTGGCATACCTGGAAGAGGAG
59.86
153
PCR template sequence:
GTGTTTACTAGGAGCCAATGGGAATATCCTTTTGCACCTTAATCCTCAGAAGCCAGGAGCAATTGATAACCAGCCACTAGTAACTCAGGAACCAGTAAAGGTGGGCCATCTTACTCTTGAAGACTATCAGATATGGAGTGTGAAAAATGTTCTTGCCAATGAGTTTTTGAATCTCCTCTTCCAGGTATGCCACATAGTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-217-5p(1), mmu-miR-24-3p(1), mmu-miR-136-5p(1), mmu-miR-182-5p(1), mmu-miR-344d-3p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
GGAACCAGTAAAGGTGGGCCATCTTA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUSP32_618_ORF_1
0.8792
0.02957498
0.979922329
1026
ORF sequence:
ATGCAGTGGTGGCAACAGTGGAAAGAATATGTCAAATATGATGCCAGCCCTGTTGTAATTGAGCCATCATCTGTTCTAAATGGAGGAAAATTTTCATTTGGAACAGCAGCACATCCTATAGAGCATGGAGAAGATAGAATCAGTAATAACCTTGGTTATATGAATACCACAGAAGAGAAATACTCAGACAACATTTCTTCTGCATCTGAAGCCTCAGAGTCTACTGGCAGTGGCTTTCTGTATTCTGGCACACCAGGGGCAGATATGTGCTTTGCAAGGCAGCATAACACTTCTGACAATAACAACCAGTGTTTACTAGGAGCCAATGGGAATATCCTTTTGCACCTTAATCCTCAGAAGCCAGGAGCAATTGATAACCAGCCACTAGTAACTCAGGAACCAGTAAAGGTGGGCCATCTTACTCTTGAAGACTATCAGATATGGAGTGTGAAAAATGTTCTTGCCAATGAGTTTTTGAATCTCCTCTTCCAGGTATGCCACATAGTTCTGGGGTTAAGACCAGCTACTCCAGAAGAAGAAGGACAAATTATTAGAGGATGGTTGGAACGAGAAAGCAGGTATGGTCTGCAGCCAGGACACAATTGGTTTATCATCTCCATGCAGTGGTGGCAACAGTGGAAAGAATATGTCAAATATGATGCCAGCCCTGTTGTAATTGAGCCATCATCTGTTCTAAATGGAGGAAAATTTTCATTTGGAACAGCAGCACATCCTATAGAGCATGGAGAAGATAGAATCAGTAATAACCTTGGTTATATGAATACCACAGAAGAGAAATACTCAGACAACATTTCTTCTGCATCTGAAGCCTCAGAGTCTACTGGCAGTGGCTTTCTGTATTCTGGCACACCAGGGGCAGATATGTGCTTTGCAAGGCAGCATAACACTTCTGACAATAACAACCAGTGTTTACTAGGAGCCAATGGGAATATCCTTTTGCACCTTAATCCTCAGAAGCCAGGAGCAATTGATAACCAGCCACTAGTAACTCAGGAACCAGTAAAG
Peptide sequence:
MQWWQQWKEYVKYDASPVVIEPSSVLNGGKFSFGTAAHPIEHGEDRISNNLGYMNTTEEKYSDNISSASEASESTGSGFLYSGTPGADMCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKVGHLTLEDYQIWSVKNVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQPGHNWFIISMQWWQQWKEYVKYDASPVVIEPSSVLNGGKFSFGTAAHPIEHGEDRISNNLGYMNTTEEKYSDNISSASEASESTGSGFLYSGTPGADMCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVK
IRESfinder result
IRESfinder score
0.664374337
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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