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mmu_circUsp32_327
Basic information of circular RNA
PanCircBase ID:
mmu_circUsp32_327
mm39_Chr. Position ID:
chr11|84912981|84913731|-
Gene name:
Usp32
Transcript:
ENSMUST00000108075.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
24,23
Exon/intron offsets:
0,575
Genomic length:
750
Exon/intron sizes in nt:
1,52,175
Flanking exon/introns:
chr11:84910702-84912981|chr11:84913731-84916344
Splice length:
327
Other database IDs:
CIRCpedia: MMU_CIRCpedia_31925, circAtlas: mmu-Usp32_0051
Human Ortholog IDs:
Mature spliced Sequence:
GCCTGGGACAACCATCTAAGGAGAAATAGATCAATTGTTGTGGACTTGTTCCATGGTCAGCTAAGATCCCAAGTCAAATGCAAGACATGTGGGCATATAAGTGTCCGATTCGACCCTTTTAATTTTTTGTCTTTACCACTACCAATGGACAGTTACATGCATTTAGAAATAACAGTAATTAAATTAGATGGTACTACCCCCATACGATATGGACTAAGACTGAATATGGATGAAAAGTATACAGGTTTGAAAAAGCAGCTGAGTGATCTCTGTGGACTTAAATCAGAACAAATCCTTTTTGCAGAAGTGCATAGTTCAAACATAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCAGCTGAGTGATCTCTGTG
59.79
TGCCCACATGTCTTGCATTTG
60
169
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCAGCTGAGTGATCTCTGTG
59.79
GCCCACATGTCTTGCATTTGA
59.73
168
PCR template sequence:
GGATGAAAAGTATACAGGTTTGAAAAAGCAGCTGAGTGATCTCTGTGGACTTAAATCAGAACAAATCCTTTTTGCAGAAGTGCATAGTTCAAACATAAAGGCCTGGGACAACCATCTAAGGAGAAATAGATCAATTGTTGTGGACTTGTTCCATGGTCAGCTAAGATCCCAAGTCAAATGCAAGACATGTGGGCATATAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-411-3p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTCAAACATAAAGGCCTGGGACAACC
siRNA sets
GTTGTCCCAGGCCTTTATG, TTGTCCCAGGCCTTTATGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUSP32_327_ORF_1
0.6127
-0.074967454
0.321866981
510
ORF sequence:
ATGGACAGTTACATGCATTTAGAAATAACAGTAATTAAATTAGATGGTACTACCCCCATACGATATGGACTAAGACTGAATATGGATGAAAAGTATACAGGTTTGAAAAAGCAGCTGAGTGATCTCTGTGGACTTAAATCAGAACAAATCCTTTTTGCAGAAGTGCATAGTTCAAACATAAAGGCCTGGGACAACCATCTAAGGAGAAATAGATCAATTGTTGTGGACTTGTTCCATGGTCAGCTAAGATCCCAAGTCAAATGCAAGACATGTGGGCATATAAGTGTCCGATTCGACCCTTTTAATTTTTTGTCTTTACCACTACCAATGGACAGTTACATGCATTTAGAAATAACAGTAATTAAATTAGATGGTACTACCCCCATACGATATGGACTAAGACTGAATATGGATGAAAAGTATACAGGTTTGAAAAAGCAGCTGAGTGATCTCTGTGGACTTAAATCAGAACAAATCCTTTTTGCAGAAGTGCATAGTTCAAACATAAAG
Peptide sequence:
MDSYMHLEITVIKLDGTTPIRYGLRLNMDEKYTGLKKQLSDLCGLKSEQILFAEVHSSNIKAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPIRYGLRLNMDEKYTGLKKQLSDLCGLKSEQILFAEVHSSNIK
IRESfinder result
IRESfinder score
0.822009496
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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