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mmu_circUsp32_2438
Basic information of circular RNA
PanCircBase ID:
mmu_circUsp32_2438
mm39_Chr. Position ID:
chr11|84908455|84944875|-
Gene name:
Usp32
Transcript:
ENSMUST00000108075.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
19
Exon/intron indexes:
26,25,24,23,22,21,20,19,18,17,16,15,14,13,12,11,10,9,8
Exon/intron offsets:
0,2135,4526,5101,7889,9146,9471,11431,12853,14439,18892,21571,22365,24858,27080,32757,33561,36018,36304
Genomic length:
36420
Exon/intron sizes in nt:
2,12,11,21,52,17,51,74,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr11:84900969-84908455|chr11:84944875-84946591
Splice length:
2438
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTTGCTTCAAGGTGTTTGATGTTGACCGTGATGGAGTTCTCTCCAGGGTTGAACTGAAAGACATGGTAGTTGCACTTTTGGAGGTCTGGAAAGATAACCGCACTGATGATATTCCTGAATTGCATATGGATTTGTCAGACATTGTAGAAAGGATATTGAATGCACATGATACCACAAAGGTGGGCCATCTTACTCTTGAAGACTATCAGATATGGAGTGTGAAAAATGTTCTTGCCAATGAGTTTTTGAATCTCCTCTTCCAGGTATGCCACATAGTTCTGGGGTTAAGACCAGCTACTCCAGAAGAAGAAGGACAAATTATTAGAGGATGGTTGGAACGAGAAAGCAGGTATGGTCTGCAGCCAGGACACAATTGGTTTATCATCTCCATGCAGTGGTGGCAACAGTGGAAAGAATATGTCAAATATGATGCCAGCCCTGTTGTAATTGAGCCATCATCTGTTCTAAATGGAGGAAAATTTTCATTTGGAACAGCAGCACATCCTATAGAGCATGGAGAAGATAGAATCAGTAATAACCTTGGTTATATGAATACCACAGAAGAGAAATACTCAGACAACATTTCTTCTGCATCTGAAGCCTCAGAGTCTACTGGCAGTGGCTTTCTGTATTCTGGCACACCAGGGGCAGATATGTGCTTTGCAAGGCAGCATAACACTTCTGACAATAACAACCAGTGTTTACTAGGAGCCAATGGGAATATCCTTTTGCACCTTAATCCTCAGAAGCCAGGAGCAATTGATAACCAGCCACTAGTAACTCAGGAACCAGTAAAGGCTACATCATTAACACTAGAAGGAGGGCGATTAAAACGAACTCCACAGCTAATCCATGGAAGAGATTATGAAATGGTCCCAGAACCCGTGTGGAGAGCACTTTATCATTGGTATGGATCAAACCTGGCCCTTCCTAGACCAGTAATCAAGAATAGCAAAACGGACATCCCAGAACTAGAATTATTTCCTCGCTATCTTCTCTTCCTTCGACAGCAGCCTGCCACACGGACACAGCAATCTAACATCTGGGTGAATATGGGAAATGTACCCTCTCCAAATGCGCCTCTAAAGCGAGTGTTAGCCTATACAGGCTGTTTTAGTAGAATGCAAACCATCAAGGAAATTCATGAATATCTGTCTCAAAGGCTTCGCATTAAAGAGGAAGATATGCGGCTGTGGCTGTACAATAGTGAGAACTACCTGACTCTTCTGGATGATGAGGATCACAGATTGGAATATTTGAAAATCCAGGATGAGCAGCACCTGGTAATTGAAGTTCGAAACAAAGATATGAGTTGGCCTGAAGAAATGTCTTTTATAGCAAACAGTAGCAAAATAGATAGACATAAAGTTCCCACAGAAAAGGGCGCCACAGGTCTGAGTAACCTGGGAAACACATGCTTCATGAACTCAAGCATCCAGTGTGTCAGCAATACACAACCACTGACACAGTACTTTATCTCAGGGAGGCATCTTTATGAACTCAACAGGACAAATCCCATTGGTATGAAAGGCCATATGGCTAAATGTTATGGCGATTTGGTGCAAGAACTTTGGAGTGGAACTCAGAAGAATGTTGCCCCCTTAAAGCTCCGGTGGACCATAGCAAAATATGCTCCCAGGTTTAATGGATTCCAGCAACAAGACTCCCAAGAACTTCTGGCTTTCCTCTTGGATGGTCTTCATGAAGATCTCAACCGAGTCCACGAGAAACCATATGTAGAACTAAAAGACAGTGACGGCCGACCCGACTGGGAAGTAGCTGCAGAGGCCTGGGACAACCATCTAAGGAGAAATAGATCAATTGTTGTGGACTTGTTCCATGGTCAGCTAAGATCCCAAGTCAAATGCAAGACATGTGGGCATATAAGTGTCCGATTCGACCCTTTTAATTTTTTGTCTTTACCACTACCAATGGACAGTTACATGCATTTAGAAATAACAGTAATTAAATTAGATGGTACTACCCCCATACGATATGGACTAAGACTGAATATGGATGAAAAGTATACAGGTTTGAAAAAGCAGCTGAGTGATCTCTGTGGACTTAAATCAGAACAAATCCTTTTTGCAGAAGTGCATAGTTCAAACATAAAGAACTTCCCTCAGGACAACCAAAAAGTTCGACTCTCAGTGAGTGGATTTTTGTGTGCATTTGAAATTCCTATTCCTGCATCTCCAGTTTCAGCTTGCAGTCCAATTCAAACAGATTGCTCTTCTTCTCCATCTACAAATGGACTGTTTACCTTGACTACCAATGGGGATCTGCCTCGACCTATATTCATCCCCAATGGAATGCCAAATACAGTTGTACCATGTGGAACTGAGAAGAATGTCACAAATGGAATAGTGAATGGTCATATGCCGCCTCTTCCTGATGACCCCTTTACAGGCTACATCATTGCAGTCCACCGAAAAATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGTCATATGCCGCCTCTTC
59.89
TCCAGACCTCCAAAAGTGCA
59.16
157
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TATGCCGCCTCTTCCTGATG
59.61
TCCAGACCTCCAAAAGTGCA
59.16
151
PCR template sequence:
AACTGAGAAGAATGTCACAAATGGAATAGTGAATGGTCATATGCCGCCTCTTCCTGATGACCCCTTTACAGGCTACATCATTGCAGTCCACCGAAAAATGTTTGCTTCAAGGTGTTTGATGTTGACCGTGATGGAGTTCTCTCCAGGGTTGAACTGAAAGACATGGTAGTTGCACTTTTGGAGGTCTGGAAAGATAACCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
27
Interacting miRNAs (#binding sites)
mmu-miR-106a-5p(2), mmu-miR-17-5p(2), mmu-miR-20b-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-135a-5p(1), mmu-miR-30e-5p(1), mmu-miR-106b-5p(1), mmu-miR-146a-5p(1), mmu-miR-217-5p(1), mmu-miR-196a-5p(1), mmu-miR-143-3p(1), mmu-miR-24-3p(1), mmu-miR-136-5p(1), mmu-miR-411-3p(1), mmu-miR-182-5p(1), mmu-miR-301b-3p(1), mmu-miR-721(1), mmu-miR-301a-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-1198-5p(1), mmu-miR-344d-3p(1), mmu-miR-207(1), mmu-miR-670-5p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCACCGAAAAATGTTTGCTTCAAGGT
siRNA sets
AAGCAAACATTTTTCGGTG, CTTGAAGCAAACATTTTTC, AGCAAACATTTTTCGGTGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUSP32_2438_ORF_1
0.7505
0.045535858
0.999998966
2433
ORF sequence:
ATGGTAGTTGCACTTTTGGAGGTCTGGAAAGATAACCGCACTGATGATATTCCTGAATTGCATATGGATTTGTCAGACATTGTAGAAAGGATATTGAATGCACATGATACCACAAAGGTGGGCCATCTTACTCTTGAAGACTATCAGATATGGAGTGTGAAAAATGTTCTTGCCAATGAGTTTTTGAATCTCCTCTTCCAGGTATGCCACATAGTTCTGGGGTTAAGACCAGCTACTCCAGAAGAAGAAGGACAAATTATTAGAGGATGGTTGGAACGAGAAAGCAGGTATGGTCTGCAGCCAGGACACAATTGGTTTATCATCTCCATGCAGTGGTGGCAACAGTGGAAAGAATATGTCAAATATGATGCCAGCCCTGTTGTAATTGAGCCATCATCTGTTCTAAATGGAGGAAAATTTTCATTTGGAACAGCAGCACATCCTATAGAGCATGGAGAAGATAGAATCAGTAATAACCTTGGTTATATGAATACCACAGAAGAGAAATACTCAGACAACATTTCTTCTGCATCTGAAGCCTCAGAGTCTACTGGCAGTGGCTTTCTGTATTCTGGCACACCAGGGGCAGATATGTGCTTTGCAAGGCAGCATAACACTTCTGACAATAACAACCAGTGTTTACTAGGAGCCAATGGGAATATCCTTTTGCACCTTAATCCTCAGAAGCCAGGAGCAATTGATAACCAGCCACTAGTAACTCAGGAACCAGTAAAGGCTACATCATTAACACTAGAAGGAGGGCGATTAAAACGAACTCCACAGCTAATCCATGGAAGAGATTATGAAATGGTCCCAGAACCCGTGTGGAGAGCACTTTATCATTGGTATGGATCAAACCTGGCCCTTCCTAGACCAGTAATCAAGAATAGCAAAACGGACATCCCAGAACTAGAATTATTTCCTCGCTATCTTCTCTTCCTTCGACAGCAGCCTGCCACACGGACACAGCAATCTAACATCTGGGTGAATATGGGAAATGTACCCTCTCCAAATGCGCCTCTAAAGCGAGTGTTAGCCTATACAGGCTGTTTTAGTAGAATGCAAACCATCAAGGAAATTCATGAATATCTGTCTCAAAGGCTTCGCATTAAAGAGGAAGATATGCGGCTGTGGCTGTACAATAGTGAGAACTACCTGACTCTTCTGGATGATGAGGATCACAGATTGGAATATTTGAAAATCCAGGATGAGCAGCACCTGGTAATTGAAGTTCGAAACAAAGATATGAGTTGGCCTGAAGAAATGTCTTTTATAGCAAACAGTAGCAAAATAGATAGACATAAAGTTCCCACAGAAAAGGGCGCCACAGGTCTGAGTAACCTGGGAAACACATGCTTCATGAACTCAAGCATCCAGTGTGTCAGCAATACACAACCACTGACACAGTACTTTATCTCAGGGAGGCATCTTTATGAACTCAACAGGACAAATCCCATTGGTATGAAAGGCCATATGGCTAAATGTTATGGCGATTTGGTGCAAGAACTTTGGAGTGGAACTCAGAAGAATGTTGCCCCCTTAAAGCTCCGGTGGACCATAGCAAAATATGCTCCCAGGTTTAATGGATTCCAGCAACAAGACTCCCAAGAACTTCTGGCTTTCCTCTTGGATGGTCTTCATGAAGATCTCAACCGAGTCCACGAGAAACCATATGTAGAACTAAAAGACAGTGACGGCCGACCCGACTGGGAAGTAGCTGCAGAGGCCTGGGACAACCATCTAAGGAGAAATAGATCAATTGTTGTGGACTTGTTCCATGGTCAGCTAAGATCCCAAGTCAAATGCAAGACATGTGGGCATATAAGTGTCCGATTCGACCCTTTTAATTTTTTGTCTTTACCACTACCAATGGACAGTTACATGCATTTAGAAATAACAGTAATTAAATTAGATGGTACTACCCCCATACGATATGGACTAAGACTGAATATGGATGAAAAGTATACAGGTTTGAAAAAGCAGCTGAGTGATCTCTGTGGACTTAAATCAGAACAAATCCTTTTTGCAGAAGTGCATAGTTCAAACATAAAGAACTTCCCTCAGGACAACCAAAAAGTTCGACTCTCAGTGAGTGGATTTTTGTGTGCATTTGAAATTCCTATTCCTGCATCTCCAGTTTCAGCTTGCAGTCCAATTCAAACAGATTGCTCTTCTTCTCCATCTACAAATGGACTGTTTACCTTGACTACCAATGGGGATCTGCCTCGACCTATATTCATCCCCAATGGAATGCCAAATACAGTTGTACCATGTGGAACTGAGAAGAATGTCACAAATGGAATAGTGAATGGTCATATGCCGCCTCTTCCTGATGACCCCTTTACAGGCTACATCATTGCAGTCCACCGAAAAATGTTTGCTTCAAGGTGTTTGATGTTGACCGTGATGGAGTTCTCTCCAGGGTTGAACTGA
Peptide sequence:
MVVALLEVWKDNRTDDIPELHMDLSDIVERILNAHDTTKVGHLTLEDYQIWSVKNVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQPGHNWFIISMQWWQQWKEYVKYDASPVVIEPSSVLNGGKFSFGTAAHPIEHGEDRISNNLGYMNTTEEKYSDNISSASEASESTGSGFLYSGTPGADMCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGSNLALPRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHRLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPIRYGLRLNMDEKYTGLKKQLSDLCGLKSEQILFAEVHSSNIKNFPQDNQKVRLSVSGFLCAFEIPIPASPVSACSPIQTDCSSSPSTNGLFTLTTNGDLPRPIFIPNGMPNTVVPCGTEKNVTNGIVNGHMPPLPDDPFTGYIIAVHRKMFASRCLMLTVMEFSPGLN
IRESfinder result
IRESfinder score
0.656589812
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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