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mmu_circUsp32_1789
Basic information of circular RNA
PanCircBase ID:
mmu_circUsp32_1789
mm39_Chr. Position ID:
chr11|84912981|84935638|-
Gene name:
Usp32
Transcript:
ENSMUST00000108075.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
13
Exon/intron indexes:
24,23,22,21,20,19,18,17,16,15,14,13,12
Exon/intron offsets:
0,575,3363,4620,4945,6905,8327,9913,14366,17045,17839,20332,22554
Genomic length:
22657
Exon/intron sizes in nt:
15,21,75,17,41,06,13,90,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr11:84910702-84912981|chr11:84935638-84941212
Splice length:
1789
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AGGATGGTTGGAACGAGAAAGCAGGTATGGTCTGCAGCCAGGACACAATTGGTTTATCATCTCCATGCAGTGGTGGCAACAGTGGAAAGAATATGTCAAATATGATGCCAGCCCTGTTGTAATTGAGCCATCATCTGTTCTAAATGGAGGAAAATTTTCATTTGGAACAGCAGCACATCCTATAGAGCATGGAGAAGATAGAATCAGTAATAACCTTGGTTATATGAATACCACAGAAGAGAAATACTCAGACAACATTTCTTCTGCATCTGAAGCCTCAGAGTCTACTGGCAGTGGCTTTCTGTATTCTGGCACACCAGGGGCAGATATGTGCTTTGCAAGGCAGCATAACACTTCTGACAATAACAACCAGTGTTTACTAGGAGCCAATGGGAATATCCTTTTGCACCTTAATCCTCAGAAGCCAGGAGCAATTGATAACCAGCCACTAGTAACTCAGGAACCAGTAAAGGCTACATCATTAACACTAGAAGGAGGGCGATTAAAACGAACTCCACAGCTAATCCATGGAAGAGATTATGAAATGGTCCCAGAACCCGTGTGGAGAGCACTTTATCATTGGTATGGATCAAACCTGGCCCTTCCTAGACCAGTAATCAAGAATAGCAAAACGGACATCCCAGAACTAGAATTATTTCCTCGCTATCTTCTCTTCCTTCGACAGCAGCCTGCCACACGGACACAGCAATCTAACATCTGGGTGAATATGGGAAATGTACCCTCTCCAAATGCGCCTCTAAAGCGAGTGTTAGCCTATACAGGCTGTTTTAGTAGAATGCAAACCATCAAGGAAATTCATGAATATCTGTCTCAAAGGCTTCGCATTAAAGAGGAAGATATGCGGCTGTGGCTGTACAATAGTGAGAACTACCTGACTCTTCTGGATGATGAGGATCACAGATTGGAATATTTGAAAATCCAGGATGAGCAGCACCTGGTAATTGAAGTTCGAAACAAAGATATGAGTTGGCCTGAAGAAATGTCTTTTATAGCAAACAGTAGCAAAATAGATAGACATAAAGTTCCCACAGAAAAGGGCGCCACAGGTCTGAGTAACCTGGGAAACACATGCTTCATGAACTCAAGCATCCAGTGTGTCAGCAATACACAACCACTGACACAGTACTTTATCTCAGGGAGGCATCTTTATGAACTCAACAGGACAAATCCCATTGGTATGAAAGGCCATATGGCTAAATGTTATGGCGATTTGGTGCAAGAACTTTGGAGTGGAACTCAGAAGAATGTTGCCCCCTTAAAGCTCCGGTGGACCATAGCAAAATATGCTCCCAGGTTTAATGGATTCCAGCAACAAGACTCCCAAGAACTTCTGGCTTTCCTCTTGGATGGTCTTCATGAAGATCTCAACCGAGTCCACGAGAAACCATATGTAGAACTAAAAGACAGTGACGGCCGACCCGACTGGGAAGTAGCTGCAGAGGCCTGGGACAACCATCTAAGGAGAAATAGATCAATTGTTGTGGACTTGTTCCATGGTCAGCTAAGATCCCAAGTCAAATGCAAGACATGTGGGCATATAAGTGTCCGATTCGACCCTTTTAATTTTTTGTCTTTACCACTACCAATGGACAGTTACATGCATTTAGAAATAACAGTAATTAAATTAGATGGTACTACCCCCATACGATATGGACTAAGACTGAATATGGATGAAAAGTATACAGGTTTGAAAAAGCAGCTGAGTGATCTCTGTGGACTTAAATCAGAACAAATCCTTTTTGCAGAAGTGCATAGTTCAAACATAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCAGCTGAGTGATCTCTGTG
59.79
TTTCCACTGTTGCCACCACT
60.03
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCAGCTGAGTGATCTCTGTG
59.79
TCTTTCCACTGTTGCCACCA
59.74
164
PCR template sequence:
GGATGAAAAGTATACAGGTTTGAAAAAGCAGCTGAGTGATCTCTGTGGACTTAAATCAGAACAAATCCTTTTTGCAGAAGTGCATAGTTCAAACATAAAGAGGATGGTTGGAACGAGAAAGCAGGTATGGTCTGCAGCCAGGACACAATTGGTTTATCATCTCCATGCAGTGGTGGCAACAGTGGAAAGAATATGTCAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
21
Interacting miRNAs (#binding sites)
mmu-miR-20b-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-106a-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-135a-5p(1), mmu-miR-106b-5p(1), mmu-miR-217-5p(1), mmu-miR-196a-5p(1), mmu-miR-143-3p(1), mmu-miR-24-3p(1), mmu-miR-136-5p(1), mmu-miR-411-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-1198-5p(1), mmu-miR-344d-3p(1), mmu-miR-207(1), mmu-miR-670-5p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTCAAACATAAAGAGGATGGTTGGAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUSP32_1789_ORF_1
0.7902
0.036053995
0.999924857
1830
ORF sequence:
ATGCAGTGGTGGCAACAGTGGAAAGAATATGTCAAATATGATGCCAGCCCTGTTGTAATTGAGCCATCATCTGTTCTAAATGGAGGAAAATTTTCATTTGGAACAGCAGCACATCCTATAGAGCATGGAGAAGATAGAATCAGTAATAACCTTGGTTATATGAATACCACAGAAGAGAAATACTCAGACAACATTTCTTCTGCATCTGAAGCCTCAGAGTCTACTGGCAGTGGCTTTCTGTATTCTGGCACACCAGGGGCAGATATGTGCTTTGCAAGGCAGCATAACACTTCTGACAATAACAACCAGTGTTTACTAGGAGCCAATGGGAATATCCTTTTGCACCTTAATCCTCAGAAGCCAGGAGCAATTGATAACCAGCCACTAGTAACTCAGGAACCAGTAAAGGCTACATCATTAACACTAGAAGGAGGGCGATTAAAACGAACTCCACAGCTAATCCATGGAAGAGATTATGAAATGGTCCCAGAACCCGTGTGGAGAGCACTTTATCATTGGTATGGATCAAACCTGGCCCTTCCTAGACCAGTAATCAAGAATAGCAAAACGGACATCCCAGAACTAGAATTATTTCCTCGCTATCTTCTCTTCCTTCGACAGCAGCCTGCCACACGGACACAGCAATCTAACATCTGGGTGAATATGGGAAATGTACCCTCTCCAAATGCGCCTCTAAAGCGAGTGTTAGCCTATACAGGCTGTTTTAGTAGAATGCAAACCATCAAGGAAATTCATGAATATCTGTCTCAAAGGCTTCGCATTAAAGAGGAAGATATGCGGCTGTGGCTGTACAATAGTGAGAACTACCTGACTCTTCTGGATGATGAGGATCACAGATTGGAATATTTGAAAATCCAGGATGAGCAGCACCTGGTAATTGAAGTTCGAAACAAAGATATGAGTTGGCCTGAAGAAATGTCTTTTATAGCAAACAGTAGCAAAATAGATAGACATAAAGTTCCCACAGAAAAGGGCGCCACAGGTCTGAGTAACCTGGGAAACACATGCTTCATGAACTCAAGCATCCAGTGTGTCAGCAATACACAACCACTGACACAGTACTTTATCTCAGGGAGGCATCTTTATGAACTCAACAGGACAAATCCCATTGGTATGAAAGGCCATATGGCTAAATGTTATGGCGATTTGGTGCAAGAACTTTGGAGTGGAACTCAGAAGAATGTTGCCCCCTTAAAGCTCCGGTGGACCATAGCAAAATATGCTCCCAGGTTTAATGGATTCCAGCAACAAGACTCCCAAGAACTTCTGGCTTTCCTCTTGGATGGTCTTCATGAAGATCTCAACCGAGTCCACGAGAAACCATATGTAGAACTAAAAGACAGTGACGGCCGACCCGACTGGGAAGTAGCTGCAGAGGCCTGGGACAACCATCTAAGGAGAAATAGATCAATTGTTGTGGACTTGTTCCATGGTCAGCTAAGATCCCAAGTCAAATGCAAGACATGTGGGCATATAAGTGTCCGATTCGACCCTTTTAATTTTTTGTCTTTACCACTACCAATGGACAGTTACATGCATTTAGAAATAACAGTAATTAAATTAGATGGTACTACCCCCATACGATATGGACTAAGACTGAATATGGATGAAAAGTATACAGGTTTGAAAAAGCAGCTGAGTGATCTCTGTGGACTTAAATCAGAACAAATCCTTTTTGCAGAAGTGCATAGTTCAAACATAAAGAGGATGGTTGGAACGAGAAAGCAGGTATGGTCTGCAGCCAGGACACAATTGGTTTATCATCTCCATGCAGTGGTGGCAACAGTGGAAAGAATATGTCAAATATGA
Peptide sequence:
MQWWQQWKEYVKYDASPVVIEPSSVLNGGKFSFGTAAHPIEHGEDRISNNLGYMNTTEEKYSDNISSASEASESTGSGFLYSGTPGADMCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGSNLALPRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHRLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPIRYGLRLNMDEKYTGLKKQLSDLCGLKSEQILFAEVHSSNIKRMVGTRKQVWSAARTQLVYHLHAVVATVERICQI
IRESfinder result
IRESfinder score
0.651596877
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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