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mmu_circUsp32_1623
Basic information of circular RNA
PanCircBase ID:
mmu_circUsp32_1623
mm39_Chr. Position ID:
chr11|84879159|84910702|-
Gene name:
Usp32
Transcript:
ENSMUST00000108075.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
32,31,30,29,28,27,26,25
Exon/intron offsets:
0,4119,6052,17474,18659,21625,29296,31431
Genomic length:
31543
Exon/intron sizes in nt:
42,52,89,19,21,21,87,10,00,00,000
Flanking exon/introns:
chr11:84878187-84879159|chr11:84910702-84912981
Splice length:
1623
Other database IDs:
CIRCpedia: MMU_CIRCpedia_31893, circAtlas: mmu-Usp32_0081
Human Ortholog IDs:
Mature spliced Sequence:
AACTTCCCTCAGGACAACCAAAAAGTTCGACTCTCAGTGAGTGGATTTTTGTGTGCATTTGAAATTCCTATTCCTGCATCTCCAGTTTCAGCTTGCAGTCCAATTCAAACAGATTGCTCTTCTTCTCCATCTACAAATGGACTGTTTACCTTGACTACCAATGGGGATCTGCCTCGACCTATATTCATCCCCAATGGAATGCCAAATACAGTTGTACCATGTGGAACTGAGAAGAATGTCACAAATGGAATAGTGAATGGTCATATGCCGCCTCTTCCTGATGACCCCTTTACAGGCTACATCATTGCAGTCCACCGAAAAATGATGAGGACAGAACTGTATTTCCTGTCATCTCAGAAGAATCGCCCCAGTCTCTTTGGGATGCCACTGATTGTTCCATGTACTGTACATACCCGGAAAAAAGATCTCTATGATGCAGTTTGGATTCAAGTATCTCGATTAGCCAGTCCGCTCCCACCTCAAGAAGCTAGCAACCATGCACAGGATTGTGATGACAGCATGGGCTATCAATATCCATTCACTCTCCGAGTTGTGCAGAAAGATGGAAACTCCTGTGCCTGGTGTCCATGGTATAGATTTTGCAGAGGCTGTAAAATCGATTGTGGAGAAGACAGAGCTTTCATTGGAAATGCCTGTATTGCTGTGGATTGGGACCCTACAGCCCTTCACCTCCGCTATCAAACATCACAGGAAAGAGTTGTAGAGGAGCATGAAAGTGTGGAACAGAGTCGGCGAGCACAAGCTGAGCCCATCAACCTGGACAGCTGTCTCCGTGCCTTCACCAGTGAGGAGGAACTGGGGGAAAATGAGATGTACTACTGCTCCAAATGTAAAACACACTGTTTGGCAACCAAGAAACTGGACCTCTGGAGGCTTCCTCCAATCCTGATTATTCACCTTAAACGATTTCAGTTTGTAAATGGACGGTGGATAAAATCACAGAAAATTGTTAAATTTCCTCGTGAAAGTTTTGACCCTAGTGCTTTTTTAGTACCAAGAGACCCAACTCTTTGCCAGCATAAACCACTCACACCCCAGGGGGATGACTTCTCTGAGCTAAGGATCCCAGCAGGAGATGTGAAGAAAGTGGATATCCAGAGCTCAGCAGGGGAGGAGGATGTGCTCCTGAGCAAGAGCCCATCTTCACTCAGTGCAAATGTCACCAGTAGCCCAAAAGGTTCACCTTCTTCATCAAGAAAAAGTGGAGCCAGCTGTCCCTCCAGCAAAAATAGCAGCCCTAACAGCAGCCCACGGACCTTGGGGAGGAACAAAGGGAGGCTCCGGCTGCCTCAAATTGGCAGCAAAAATAAATTGTCCAATAGTAAGGAGAATTTGGATACCAGCAAAGAGAATGGGGCTGGGCAGATCTGTGAACTGGCTGACACCTTGAACCGAAGACATGTATTGGGAGGCAGCCAGCCTGAGCTGGTCACTCCTCTGGATCATGAGATCACTTTGGCTAATGGATTTCTTTATGAGCATGAGGCATGTGGCAATGGCTATAGCAATGGTCAGCTTGGAAACCATAGTGAAGAAGACAGCACTGATGACCAAAGAGAAGAAACACACAGTAAACCTATTTATAATCTATATGCAATTTCG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGAAGACAGCACTGATGACCA
59.03
GCTGAAACTGGAGATGCAGG
58.91
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGAAGACAGCACTGATGACCA
59.03
GCAAGCTGAAACTGGAGATGC
60.14
166
PCR template sequence:
TAGCAATGGTCAGCTTGGAAACCATAGTGAAGAAGACAGCACTGATGACCAAAGAGAAGAAACACACAGTAAACCTATTTATAATCTATATGCAATTTCGAACTTCCCTCAGGACAACCAAAAAGTTCGACTCTCAGTGAGTGGATTTTTGTGTGCATTTGAAATTCCTATTCCTGCATCTCCAGTTTCAGCTTGCAGTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
18
Interacting miRNAs (#binding sites)
mmu-miR-136-5p(2), mmu-miR-370-3p(2), mmu-miR-9-5p(1), mmu-miR-150-5p(1), mmu-miR-30e-5p(1), mmu-miR-101a-3p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-217-5p(1), mmu-miR-101b-3p(1), mmu-miR-324-5p(1), mmu-miR-149-5p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-705(1), mmu-miR-29b-2-5p(1), mmu-miR-697(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATATGCAATTTCGAACTTCCCTCAGG
siRNA sets
AGGGAAGTTCGAAATTGCA, GAGGGAAGTTCGAAATTGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUSP32_1623_ORF_1
0.9258
0.110142462
0.999999993
3048
ORF sequence:
ATGCCAAATACAGTTGTACCATGTGGAACTGAGAAGAATGTCACAAATGGAATAGTGAATGGTCATATGCCGCCTCTTCCTGATGACCCCTTTACAGGCTACATCATTGCAGTCCACCGAAAAATGATGAGGACAGAACTGTATTTCCTGTCATCTCAGAAGAATCGCCCCAGTCTCTTTGGGATGCCACTGATTGTTCCATGTACTGTACATACCCGGAAAAAAGATCTCTATGATGCAGTTTGGATTCAAGTATCTCGATTAGCCAGTCCGCTCCCACCTCAAGAAGCTAGCAACCATGCACAGGATTGTGATGACAGCATGGGCTATCAATATCCATTCACTCTCCGAGTTGTGCAGAAAGATGGAAACTCCTGTGCCTGGTGTCCATGGTATAGATTTTGCAGAGGCTGTAAAATCGATTGTGGAGAAGACAGAGCTTTCATTGGAAATGCCTGTATTGCTGTGGATTGGGACCCTACAGCCCTTCACCTCCGCTATCAAACATCACAGGAAAGAGTTGTAGAGGAGCATGAAAGTGTGGAACAGAGTCGGCGAGCACAAGCTGAGCCCATCAACCTGGACAGCTGTCTCCGTGCCTTCACCAGTGAGGAGGAACTGGGGGAAAATGAGATGTACTACTGCTCCAAATGTAAAACACACTGTTTGGCAACCAAGAAACTGGACCTCTGGAGGCTTCCTCCAATCCTGATTATTCACCTTAAACGATTTCAGTTTGTAAATGGACGGTGGATAAAATCACAGAAAATTGTTAAATTTCCTCGTGAAAGTTTTGACCCTAGTGCTTTTTTAGTACCAAGAGACCCAACTCTTTGCCAGCATAAACCACTCACACCCCAGGGGGATGACTTCTCTGAGCTAAGGATCCCAGCAGGAGATGTGAAGAAAGTGGATATCCAGAGCTCAGCAGGGGAGGAGGATGTGCTCCTGAGCAAGAGCCCATCTTCACTCAGTGCAAATGTCACCAGTAGCCCAAAAGGTTCACCTTCTTCATCAAGAAAAAGTGGAGCCAGCTGTCCCTCCAGCAAAAATAGCAGCCCTAACAGCAGCCCACGGACCTTGGGGAGGAACAAAGGGAGGCTCCGGCTGCCTCAAATTGGCAGCAAAAATAAATTGTCCAATAGTAAGGAGAATTTGGATACCAGCAAAGAGAATGGGGCTGGGCAGATCTGTGAACTGGCTGACACCTTGAACCGAAGACATGTATTGGGAGGCAGCCAGCCTGAGCTGGTCACTCCTCTGGATCATGAGATCACTTTGGCTAATGGATTTCTTTATGAGCATGAGGCATGTGGCAATGGCTATAGCAATGGTCAGCTTGGAAACCATAGTGAAGAAGACAGCACTGATGACCAAAGAGAAGAAACACACAGTAAACCTATTTATAATCTATATGCAATTTCGAACTTCCCTCAGGACAACCAAAAAGTTCGACTCTCAGTGAGTGGATTTTTGTGTGCATTTGAAATTCCTATTCCTGCATCTCCAGTTTCAGCTTGCAGTCCAATTCAAACAGATTGCTCTTCTTCTCCATCTACAAATGGACTGTTTACCTTGACTACCAATGGGGATCTGCCTCGACCTATATTCATCCCCAATGGAATGCCAAATACAGTTGTACCATGTGGAACTGAGAAGAATGTCACAAATGGAATAGTGAATGGTCATATGCCGCCTCTTCCTGATGACCCCTTTACAGGCTACATCATTGCAGTCCACCGAAAAATGATGAGGACAGAACTGTATTTCCTGTCATCTCAGAAGAATCGCCCCAGTCTCTTTGGGATGCCACTGATTGTTCCATGTACTGTACATACCCGGAAAAAAGATCTCTATGATGCAGTTTGGATTCAAGTATCTCGATTAGCCAGTCCGCTCCCACCTCAAGAAGCTAGCAACCATGCACAGGATTGTGATGACAGCATGGGCTATCAATATCCATTCACTCTCCGAGTTGTGCAGAAAGATGGAAACTCCTGTGCCTGGTGTCCATGGTATAGATTTTGCAGAGGCTGTAAAATCGATTGTGGAGAAGACAGAGCTTTCATTGGAAATGCCTGTATTGCTGTGGATTGGGACCCTACAGCCCTTCACCTCCGCTATCAAACATCACAGGAAAGAGTTGTAGAGGAGCATGAAAGTGTGGAACAGAGTCGGCGAGCACAAGCTGAGCCCATCAACCTGGACAGCTGTCTCCGTGCCTTCACCAGTGAGGAGGAACTGGGGGAAAATGAGATGTACTACTGCTCCAAATGTAAAACACACTGTTTGGCAACCAAGAAACTGGACCTCTGGAGGCTTCCTCCAATCCTGATTATTCACCTTAAACGATTTCAGTTTGTAAATGGACGGTGGATAAAATCACAGAAAATTGTTAAATTTCCTCGTGAAAGTTTTGACCCTAGTGCTTTTTTAGTACCAAGAGACCCAACTCTTTGCCAGCATAAACCACTCACACCCCAGGGGGATGACTTCTCTGAGCTAAGGATCCCAGCAGGAGATGTGAAGAAAGTGGATATCCAGAGCTCAGCAGGGGAGGAGGATGTGCTCCTGAGCAAGAGCCCATCTTCACTCAGTGCAAATGTCACCAGTAGCCCAAAAGGTTCACCTTCTTCATCAAGAAAAAGTGGAGCCAGCTGTCCCTCCAGCAAAAATAGCAGCCCTAACAGCAGCCCACGGACCTTGGGGAGGAACAAAGGGAGGCTCCGGCTGCCTCAAATTGGCAGCAAAAATAAATTGTCCAATAGTAAGGAGAATTTGGATACCAGCAAAGAGAATGGGGCTGGGCAGATCTGTGAACTGGCTGACACCTTGAACCGAAGACATGTATTGGGAGGCAGCCAGCCTGAGCTGGTCACTCCTCTGGATCATGAGATCACTTTGGCTAATGGATTTCTTTATGAGCATGAGGCATGTGGCAATGGCTATAGCAATGGTCAGCTTGGAAACCATAGTGAAGAAGACAGCACTGATGACCAAAGAGAAGAAACACACAGTAAACCTATTTATAATCTATATGCAATTTCG
Peptide sequence:
MPNTVVPCGTEKNVTNGIVNGHMPPLPDDPFTGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFIGNACIAVDWDPTALHLRYQTSQERVVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLVPRDPTLCQHKPLTPQGDDFSELRIPAGDVKKVDIQSSAGEEDVLLSKSPSSLSANVTSSPKGSPSSSRKSGASCPSSKNSSPNSSPRTLGRNKGRLRLPQIGSKNKLSNSKENLDTSKENGAGQICELADTLNRRHVLGGSQPELVTPLDHEITLANGFLYEHEACGNGYSNGQLGNHSEEDSTDDQREETHSKPIYNLYAISNFPQDNQKVRLSVSGFLCAFEIPIPASPVSACSPIQTDCSSSPSTNGLFTLTTNGDLPRPIFIPNGMPNTVVPCGTEKNVTNGIVNGHMPPLPDDPFTGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFIGNACIAVDWDPTALHLRYQTSQERVVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLVPRDPTLCQHKPLTPQGDDFSELRIPAGDVKKVDIQSSAGEEDVLLSKSPSSLSANVTSSPKGSPSSSRKSGASCPSSKNSSPNSSPRTLGRNKGRLRLPQIGSKNKLSNSKENLDTSKENGAGQICELADTLNRRHVLGGSQPELVTPLDHEITLANGFLYEHEACGNGYSNGQLGNHSEEDSTDDQREETHSKPIYNLYAIS
IRESfinder result
IRESfinder score
0.603443245
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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